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The present study was conducted to determine the effect of different concentrations of putrescine and spermidine
at all stages of regeneration (callogenesis, somatic embryos multiplication, germination and rooting)) of date palm cultivar
Barhee. Shoot tips were eradicated from 2-3 years old offshoots, surface sterilized and inoculated onto Murashiege and
Skoog, 1962 (MS) medium supplemented with 20 mg/L 2,4-D and 3 mg/L N6-2-isopentyl adenine (2ip). Primary callus was
obtained after 24 weeks on the nutrient medium. Calli were then transferred onto fresh MS medium containing 0.0, 50, 100 or
150 mg/L of putrescine or spermidine individually. Results were recorded after 12 weeks. A significant increase in embryonic
callus fresh weights reached
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