Background: Sildenafil, a selective phosphodiesterase -5 (PDE-5) inhibitor increases cyclic guanosine monophosphate (cGMP) and amplifies many biological actions of nitric oxide (NO) which is an important mediator of gastric mucosal defense ; was evaluated in this study using different doses for its cytoprotective effect on gastric mucosal damage induced by indomethacin . We also evaluated the effect of this drug on NO production, Prostaglandin E2 (PGE2) synthesis, Myeloperoxidase (MPO) activity, and Interlukin-4 (IL-4) expression.
Methods: The study was performed between April and July 2008 in the Department of Pharmacology / College of Medicine /Baghdad University .It was conducted on 80 adult male albino rats, divided into 8 groups, the first served as a control received the vehicle, the second received indomethacin orally of 60mg/kg .The third and fourth groups were pretreated 30 minutes prior indomethacin with oral sildenafil 10 and 20mg/kg respectively. The other groups were pretreated 30 minutes prior to sildenafil (10and20mg/kg) with intraperitoneal NG-L-Arginine Methyl Ester (L-NAME) 20mg/kg with or without L-Arginine. The rats were then sacrificed after 4 hours and their stomachs were isolated and submitted to macroscopical assessment and for the measurement of the gastric PGE2, MPO, and IL-4.
Results: Sildenafil (10 and 20 mg/kg) pretreatment resulted in a significant (p<0.01) decrease in the gastric damage score. The MPO activity was significantly (p<0.01) decreased, while the level of IL-4 was significantly (p<0.01) increased. On the other hand PGE2 level was not changed. L-NAME given 30 min before 10 and 20 mg/kg sildenafil, significantly (p<0.01) abolished the protective effects of sildenafil and also reversed the effects of sildenafil on MPO activity and IL-4 levels. On the other hand addition of L-Arginine resulted in the restoration of the protective effects of sildenafil which was also reflected on the gastric damage score, MPO activity and IL-4 levels.
Conclusions: The results demonstrate that the injurious effect of indomethacin can be reduced or ameliorated by sildenafil pretreatment, and that the protective effect of sildenafil against
indomethacin was through an NO dependent pathway.
Organophosphorus insecticide and growth regulator namely Ethephon (2-chloroethylphosphonic acid) are widely used as a ripening process accelerator and a cultivation duration inhibitor. Pomegranate extract (PPE) has recently been taken into consideration due to its pharmacological effects especially those associated with renal diseases. Thus, this study aims to investigate the possible protective effect of PPE against ethephon-induced nephrotoxicity in rats. In this study four groups of adult male rats were divided into control group, PPE 400 mg/kg group, Ethephon 250 mg/kg group, and finally, PPE + Ethephon group (treated with the same dose of PPE group and Ethephon group). In the current study, kidney function parameters (KIM-1, creatin
... Show MoreBackground: Alum has been used as a treatment medication in cases of oral and gingival ulcers, and also as antiseptic mouthwash. This study aimed to examine the effects of different concentrations of Alum on inhibition zone, viability counts and adherence ability of Mutans streptococci compared with deionized water and chlorhexidine gluconate in vitro. Materials and methods: The study dealt with an in vitro study to establish a concentration of Alum mouthrinse that would have the minimum inhibitory concentration and minimum bacteriocidal concentration. The second part evaluated the anti-adherence ability of the experimental agents. Results: This study found that the antibacterial effect of Alum increases with its concentration from 50 to 1
... Show MoreThis study was done in Baghdad teaching Hospital by using developed instrument type GIOHO and included a number of patients with compressed breast thickness (7,8,9,10)cm .
The relationship between radiation dose and breast thickness was linear. All results were compared with the international standered values that measured by the International Nuctear Agency and Europeon sources ,it was found that it is in consistance or has a little difference .
The study showed that the mean absorbed dose may be determined by using TLD measurement below 10 mGy and the glandular dose was (1.45 mGy) and this can not b
... Show MoreBackground: The most widely used material for fabrication of denture base is poly methyl methacrylate, despite its popularity, the main problems associated with it as a denture base material are poor strength particularly under fatigue failure inside the patient mouth, impact failure outside the patient mouth, which are the main causes for fracture of denture, several studies was done to increase mechanical properties of denture base. The present study was conducted to evaluate and compare the effect of addition single walled carbon nanotubes in different concentrations to polymethyl methacrylate on some mechanical properties (surface hardness, surface roughness, impact strength and transverse strength). Materials and methods: Forty eight
... Show MoreObjective: To evaluate and compare the effect of mechanical surface treatment (groove, aluminum oxide particles)
with 45 degree bevel type of joint on tensile bond strength of acrylic specimens repaired by two curing methods
(microwave and water both).
Methodology: Eighty specimens (80) were prepared from pink heat cure acrylic resin. They were divided into two
main groups (40 specimen repaired by microwave energy and 40 specimens repaired by water bath method).Each
group can be divided into four subgroups of ten according to the surface treatment. The control group A was left
intact, group B received no surface treatment, group C and D received surface treatment by (groove, 50 m aluminum
oxide particles). Specimens
The infection with H. Pylori stimulates a signaling cascade that causes the generation of Cytokines and provokes Oxidative stress that is involved in the chronic inflammatory response leads to Gastric cancers. Reactive oxygen species (ROS) produce 8-Hydroxydeoxyguanosine (8-OHdG), the persistent oxidative DNA damage product. The study objective was to assess if there was a link between inflammatory cytokine levels and the presence of Oxidative DNA damage in Gastric tumor patients. In addition, evaluation of the diagnostic and prognostic value of Oxidative DNA damage and inflammatory cytokine biomarkers for Stomach cancers is being conducted. The study was accomplished on medically diagnosed Stomach cancer patients before any form of trea
... Show MoreQuercetin, one of the flavonoids family member, can be found in many vegetables, fruits, and beverages with a noticeable nutritional pharmacological properties. This study was aimed to evaluate the ability of quercetin to inhibit lipopolysaccharide (LPS) that induced lethal toxicity in vivo, and to elucidate the importance of the quercetin as an antitumor agent in breast cancer cell line MCF-7.In vivo experiments included the effect of hesperidin and LPS on the liver and spleen of male mice. In the liver, the antioxidant activity was measured by estimating the concentration of glutathione (GSH), and catalase (CAT), while in the spleen, the concentration of cytokines was measured including IL-33 and TNF-α. In vitro experiments included MTT
... Show MoreABSTRACT
This study was conducted to determine the effect of various levels of hump fat (HF) used in manufacturing of camel, beef and chicken sausage to understand the effect of (HF) on physicochemical composition sausage, Different levels of hump fat (5, 7, and 10 %) were used, physicochemical compositions like (moisture, protein, fat, Ash, water holding capacity, shrinkage, cooking loss and pH) were determined. Results of the study revealed that moisture content showed high significant differences (P≤0.01)among treatments groups, Camel sausage and beef sausage tended to have highest values while chicken sausage reported the lowest value. The study showed no significant difference (P≤0.05) among the
... Show MoreIn this study, uranium concentrations were evaluated and the annual effective dose was calculated from the consumption of bottled water and tap water used for drinking in Babylon, middle Iraq. Uranium isotopes (238U, 234U, 235U) were determined for all samples collected using the phosphorylation analyzer technique represented device (KPA). Forty-four samples were collected to cover almost all districts and regions of Babylon. Thirty tap water samples were collected from residential neighborhoods, with 14 samples from local brand bottled water. The results show that the uranium concentrations in the tap water samples ranged from 1.66 μg.L-1 to 2.64 μg.L-1, with an avera
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