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`In Situ Hybridization for Detection of Latent Epstein-Barr Virus Early Repeats(EBERS) and Mutant-P53- Tumor Suppressor Gene in Patients with Non- Hodgkins Lymphoma
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Back ground: Epstein- Barr virus (EBV) is a ubiquitous in that infecting more than 90% of adult population worldwide. Recently, EBV has been linked to the development of variety of human malignancies. P53 gene is mutated in more than 50% of human cancers. Cell cycle dysregulation, measured by p53 protein expression, and latent EBV infection are important in the pathogenesis of Non-Hodgkin’s lymphomas.
Objective: To analyze the distribution and impact of concordant p53 expression and latent EBV infection on a group of B & T cell types of NHL.
Materials and Methods: Forty (40) formalin-fixed, paraffin embedded tissue blocks were obtained from lymph nodes biopsies related to patients with NHL.
In addition, biopsies of twenty (20) lymph nodes autopsies were included as apparently normal control group. The clinico- pathological criteria of NHL cases were assessed and In Situ Hybridization( ISH ) techniques for mutant p53 and Epstein-Barr Early Repeats(EBERs) detection were performed.
Results: The percentage of EBV-ISH reaction results in the total group of NHL was (60%), where its percentage in NHL, B-cell type was (58.6%)and in NHL, T-cell type (63.7%). None of the control group showed EBV-ISH reaction. Statistical analysis showed significant difference between these groups (p<0.05). The percentage of B-cell lymphoma with moderate signal score for EBV-ISH test was (41.4%) while with strong scoring was (17.2%). In the T-cell lymphoma, moderate signal scoring was (18.2%) while strong scoring was(45.5%). Statistically, they showed no significant differences (p> 0.05). Mutant p53 gene was observed in 60% of NHLs and dual positivity of EBV and p53-ISH was found in 42.5 % of cases. Regarding the grades, it was found that the higher level of p53 expression was observed in low grade tumors (39.3% ; 11out of 28) NHL.
Conclusion: The present findings indicate that cell cycle dysregulation and EBV-related transformation are important in the pathogenesis of NHL.

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Publication Date
Sun Jan 03 2016
Journal Name
Journal Of The Faculty Of Medicine Baghdad
The effect of pregnancy on peak expiratory flow rate in comparison with non-pregnant Iraqi women Sample in MARJAN Teaching Hospital
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Background: Profound alterations of respiratory function physiology accompany pregnancy; these conditions contribute to many of the disorders of the lung during pregnancy. The adaptive changes during the gravid period are designed to support maternal and fetal well-being during the special stresses of fetal growth and parturition Peak.
Objective: This study aimed to know the effect of pregnancy on peak expiratory flow rate in comparison with non-pregnant Iraqi women.
Method: This study was conducted on 255 healthy female at their reproductive ages , made up of 60 pregnant female in 1st trimester , 65 in 2nd trimester , 60 in 3rd trimester and 70 non pregnant as control group.
Results: There were a significant negative relation b

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Publication Date
Sun Oct 29 2023
Journal Name
Iraqi National Journal Of Nursing Specialties
Assessment of Quality of life for Patients with Myocardial Infarction
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Objectives: To identify quality of life (QOL) in Myocardial Infarction (MI) patients, and to find out the
relationship between QOL in MI patients and demographic characteristics.
Methodology: A descriptive colTelation study which utilized an assessment approach. The study was carried out
from March 2007 through November 2007 in order to assess the quality of life for patients with myocardial
infarction. A purposive "non-probability" sample of (75) patients with myocardial infarction who were attending
to Baquba General Hospita`l through their visits to that hospital. A questionnaire was adapted and developed
from the World Health Organization Quality of Life Scale (1998). The questionnaire was designed and
consisted

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Publication Date
Tue May 30 2023
Journal Name
Iraqi Journal Of Science
Ltb4r Gene Expression in Chronic Myeloid Leukemia in Iraq
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     The current study was carried out to explore gene expression of the LTB4R gene with the development of chronic myeloid leukemia (CML) in Iraqi patients. The differences in the expression of this gene between patients and healthy controls were studied. The correlation of gender and age with CML patients compared with controls was included as well as the correlation of gene expression folding 2-ΔΔCt of LTB4R with clinical parameters (WBC, RBC, haemoglobin, platelets, and BCR-ABL gene). Results revealed significant increases in the mean of gene expression level (ΔCt) of patient groups compared to the corresponding ΔCt means in the healthy control group, the gene expression folding (2-∆∆Ct) of the L

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Publication Date
Sun Jul 02 2017
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Magnetic resonance imaging in assessment of liver lesions in patients with extrahepatic primary cancer
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Background: Liver imaging is commonly undertaken in patients with cancer history because, after lymph nodes, the liver is the most frequently involved organ by metastases
Objectives: The aim of the study was to evaluate the role of liver MRI (magnetic resonance imaging) in characterization and detection of liver lesion in patients with extrahepatic primary
Methods: this is a cross sectional study of 70 patients with extrahepatic liver primary cancer who had their treatment in oncology teaching hospital underwent routine abdominal ultrasound to detect liver lesion(s) and suspicious cases then referred to MRI which was done in Ghazi Alharri and oncology teaching hospital from the period from 1st of September 2015 to end of November 2

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Publication Date
Wed Apr 15 2020
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Virulence estimation by calculation of relative expression of NESTIN in different grades of astrocytoma from different age groups of Iraqi patients, extracted from brain tumor stem cells
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Background: Astrocytoma, is heterogeneous tumor of the nervous system and studies on the virulence of these tumors reveal that their behavior is led by small population of cells which are the brain tumor stem cells (BTSCs) that drive the continuous proliferation and self-renewal. From the many markers that annotate BTSCs, are the CD133, and NESTIN.

Objectives: Using CD133, to immunolabel BTSCs niches in paraffin sections of astrocytoma then, extraction of these cells, to calculate fold expression of NESTIN gene across the grades by real time PCR.

Materials and methods: Paraffin blocks of four grades of primary astrocytoma have been selected from three age groups from Iraq

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Publication Date
Thu Jun 30 2022
Journal Name
Iraqi Journal Of Science
Biostratigraphy of the Early Cretaceous Mauddud Formation in Ratawi Oilfield, Basrah Governorate, Southern Iraq
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      The biostratigraphy of the Early Cretaceous Mauddud Formation was studied in the Ratawi Oilfield, Basra Governorate, southern Iraq, using integrated borehole data set (core and cutting samples and well logs) in two drilled wells to analyze the biostratigraphy of the formation.  One hundred eighty-three slides for both selected wells were investigated. The formation is composed of light grey dolomitized limestone and pseudo-oolitic creamy limestone with green to bluish shale. Three biozones were discriminated, these are: Orbitolina qatarica range zone; Orbitolina sefini range zone and Orbitolina concava range zone. The age of these biozones extends to include the Late Albian (Orbitolina qatarica<

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Publication Date
Tue Jun 20 2023
Journal Name
Baghdad Science Journal
Detection of Autism Spectrum Disorder Using A 1-Dimensional Convolutional Neural Network
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Autism Spectrum Disorder, also known as ASD, is a neurodevelopmental disease that impairs speech, social interaction, and behavior. Machine learning is a field of artificial intelligence that focuses on creating algorithms that can learn patterns and make ASD classification based on input data. The results of using machine learning algorithms to categorize ASD have been inconsistent. More research is needed to improve the accuracy of the classification of ASD. To address this, deep learning such as 1D CNN has been proposed as an alternative for the classification of ASD detection. The proposed techniques are evaluated on publicly available three different ASD datasets (children, Adults, and adolescents). Results strongly suggest that 1D

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Publication Date
Thu Nov 02 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Total Protein and Total Fucose in Sera of Patients with Thyroid Dysfunction and Control
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Total protein and total fucose were determined in sera of thyroid

disorder patients.

Sera of (40) diagnosed by consultant hyperthyroidism, and 40 hypothyroidism  were analyzed  for  the above  parameter  for  control, sera of (40) normal individuals were used.

They   were   healthy   with    no  appearing   disorder   results   analysis revealed  no significant  differences  (P<0.05)  in  the (mean  Â±SD)  of total   protein   values  in   sera  of   hyper  and   hypothyroidism   were compared

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Publication Date
Thu Jan 30 2014
Journal Name
Al-kindy College Medical Journal
Change in Taste in Diabetic Patients with Facial Nerve Palsy
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Background: Bell's palsy was defined as facial weakness of lower motor neuron type caused by idiopathic facial nerve involvement outside the central nervous system without evidence of aural or more widespread neurologic disease. The cause is unclear, but the disorder occurs more commonly in diabetics.Objectives: to differentiate cases of idiopathic Bell's palsy from diabetic mononeuropathy presented with Facial nerve palsy by assessing the taste, because they differ in etiology, management & prognosis.Patients &Methods: One hundred and fifteen consecutive patients were referred for the treatment of facial palsy, from May the 5th 2012 to April 12th 2013 in Al-Kindy Teaching Hospital and The Neurosciences Hospital, in Baghdad / Ira

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Publication Date
Tue Aug 15 2023
Journal Name
Bionatura
Gene expression of a nitrogen tolerance gene ZmNR1 under the influence of different levels of nitrogen in maize
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A field experiment was carried out in the College of Agricultural Engineering Sciences - University of Baghdad, during the fall season of 2021 to find out which cultivated cultivars of maize are efficient under nitrogen fertilization. The experiment was applied according to an RCBD (split-plot design with three replications). The cultivars of the experiment (Baghdad, 5018, Sarah) supply three levels of nitrogen fertilizer, which are N1 (100 kg.N/ha), N2 (200 kg.N/ha) and N3 (300 kg.N/ha). The statistical analysis results showed the superiority of the Sarah genotype, which gave the highest value of SOD and CAT enzymes, reaching 11.59 units mg-1 and 10.76 units mg-1 . Protein sequentially, while cultivar5018 outperformed as it gave th

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