Background: targeted cancer nanotherapy represents a golden goal for nanobiotechnology to overcome the severe side effects of conventional chemotherapy. Hybrid nanoliposomes (HLs) composed of L-α-dimyristoylphosphatidylcholine (DMPC) and Polyoxyethylene (23) dodecyl ether (C12 (EO)23 ) can integrate selectively into the cancer cell membrane inducing cancer cell death.
Objectives: to assess the capacity of locally (in hose) synthesized hybrid nanoliposome to inhibit the growth of cervix cancer cells (HeLa) and induce apoptosis.
Patients and Methods: hybrid nanoliposomes(nHLs) synthesized by sonication method from a mixture of 90% mol DMPC and 10% mol C12(EO)23 in tissue culture media RPMI-1640 for 6 hours at 300W and 40ºC then filtration with 0.2μm filter. Shape and size characterized with scanning electron microscope (SEM). Viability of HeLa cell and normal lymphocytes challenged with HLs were determined using MTT assay. Induction of apoptosis in the challenged cells was examined by staining with fluorescence dye mix acridine orange/propidium iodide.
Results: synthesized nHLs were in nanozise range and selectively inhibited HeLa cells proliferation with IC50 of 0.2mM DMPC with no effect against normal lymphocytes. Apoptosis was evident in 88.24% of HeLa cells population treated with HLs.
Conclusion: synthesized nHLs may considered as promising nanotherapy, this study recommends further inspections for the mechanism of action of nHLs and their capabilities to inhibit other types of cancers both in vitro and in vivo
The presence of White Blood Cells (WBCs) in the body of human has a great role in the protection of the body against many pathogens. The recognition of the WBC is the first important step to diagnose some particular diseases. The pathologists usually use an optical microscope to recognize WBCs, but, this process is a quite tedious, time-consuming, error prone, very slow, and expensive. In addition, it needs experts with long practice in this field. For these reasons, a computer assisted diagnostic system that helps pathologists in the process of diagnosis can be effective, easy and safe. This research is devoted to develop a system based on digital image processing methods to localize WBCs nuclei. The proposed system involved a collectio
... Show MoreThe current research included obtaining the best performance specifications for a silicon device with a mono-crystalline type pn junction (pn–Si). A simulation of the device was performed by the use of a computer program in one dimension SCAPS-1D in order to reach the optimum thickness for both p and n layers and to obtain the best efficiency in performance of the pn-Si junction. The optimum device efficiency was eta (η) = 12.4236 % when the ideal thickness for the p and n layers was 5µm and 1.175µm, respectively (p=5 µm and n=1.75µm).
The research included studying the effects of different spectra of solar illumination using simulation of the device; the usual solar spectrum AM1_5 G1 sun. Spectrum
... Show MoreBackground: Deficiency in beta cell mass is the hallmark of most forms of diabetes, it is worthwhile understanding pancreatic regeneration in the context of this disease.Of crucial importance in the development of diabetes, both type I and type II, is the insufficient beta cell replication after the onset of disease.This is why we are always in search of new sources of beta cells to be generated by neogenesis to induce beta cells. In this regard, pancreatic stem or progenitor cells may offer a promising therapeutic approach for diabetes.
Methods and Materials: A total of 60 adults swiss albino rats were divided into two groups. Group I, control group (30 animals) were injected with normal saline into the
In this study, an industrial source) E-Glass fiber) and a natural source (chestnut filler) were combined to improve the properties of polyesters. Hand-layup technique was applied in this work. Polyester (UPE) were reinforced with E-glass fibers, then reinforced with nano chestnut particles. All composites were prepared with (10% wt.) of E-glass for all prepared sheet and this ratio applied for Nano chestnut composite to preparing nano hybrid composites, Nano chestnut particles were used to reinforce E-glass/UPE composites with weight ratio (3%, 6% and 9% wt.). The evaluated mechanical performances for E-glass/NCSP polyester composite were tensile strength, impact strength and hardness. The higher ultimate tensile strength, Y
... Show MoreIn this paper, numerical and experimental studies on the elastic behavior of glass fiber reinforced polymer (GFRP) with stiffeners in the GFRP section's web (to prevent local buckling) are presented. The GFRP profiles were connected to the concrete deck slab by shear connectors. Two full-scale simply supported composite beams (with and without stiffeners) were tested under impact load (three-point load) to assess its structural response. The results proved that the maximum impact force, maximum deflection, damping time, and damping ratio of the composite beam were affected by the GFRP stiffeners. The experimental results indicated that the damping ratio and deflection were diminished compare
... Show MoreBackground:
There are many techniques for face recognition which compare the desired face image with a set of faces images stored in a database. Most of these techniques fail if faces images are exposed to high-density noise. Therefore, it is necessary to find a robust method to recognize the corrupted face image with a high density noise. In this work, face recognition algorithm was suggested by using the combination of de-noising filter and PCA. Many studies have shown that PCA has ability to solve the problem of noisy images and dimensionality reduction. However, in cases where faces images are exposed to high noise, the work of PCA in removing noise is useless, therefore adding a strong filter will help to im
... Show MoreThe dynamic development of computer and software technology in recent years was accompanied by the expansion and widespread implementation of artificial intelligence (AI) based methods in many aspects of human life. A prominent field where rapid progress was observed are high‐throughput methods in biology that generate big amounts of data that need to be processed and analyzed. Therefore, AI methods are more and more applied in the biomedical field, among others for RNA‐protein binding sites prediction, DNA sequence function prediction, protein‐protein interaction prediction, or biomedical image classification. Stem cells are widely used in biomedical research, e.g., leukemia or other disease studies. Our proposed approach of
... Show MoreBackground: He-Ne laser (632.8 nm) pre-irradiation may lead to modulate the damaging effects of ionizing radiation and decreasing in radiation damage on cells, by the induction of antioxidant defence mechanisms and accelerated the repair.
Objective: To study the protection properties of He-Ne laser (632.8 nm) pre-irradiation against UV light (253.7 nm) damage on human lymphocyte blood cells and its DNA.
Subject & Methods: 27 samples were processed only for lymphocyte blood cells separation, while a 45 samples for the extracted DNA from lymphocyte. The total of 72 sampleswere employed to evaluate the influence of He-Ne laser pre- irradiation against UV-light.
Results: The results showed that He-Ne laser (632.8 nm) pre-irradia
In this paper three techniques for image compression are implemented. The proposed techniques consist of three dimension (3-D) two level discrete wavelet transform (DWT), 3-D two level discrete multi-wavelet transform (DMWT) and 3-D two level hybrid (wavelet-multiwavelet transform) technique. Daubechies and Haar are used in discrete wavelet transform and Critically Sampled preprocessing is used in discrete multi-wavelet transform. The aim is to maintain to increase the compression ratio (CR) with respect to increase the level of the transformation in case of 3-D transformation, so, the compression ratio is measured for each level. To get a good compression, the image data properties, were measured, such as, image entropy (He), percent root-
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