Summary:
Background: the immune system differentiates between the endogenous and exogenous bodies that inter the body. The break-down of blood-testis barrier results in the production of antisperm antibodies. This may occur in the case of an infection to the prostate, seminal vesicle and epididymes. Antisperm antibodies (ASA) cause sperm agglutination and affect sperm motility, viability and sperm migration in the female reproductive tract. ASA also impair fertilization process. The objective of the present work was to study the effect of prednisolone on sperm motility index (SMI), viability and sperm penetration assay (SPA) in immunologically infertile men.
Materials and methods: The semen and serum samples of 140 infertile men were examined by microagglutination test and slide agglutination test to detect ASA and sperm agglutination. Semen fliud analysis was performed to report sperm motility index (SMI), sperm viability and hypo-osmotic swelling test (HOST). Sperm penetration assay was done to record sperm penetration rate (SPR), sperm decondensation rate (SDR) and sperm penetration index (SPI). Men with positive ASA were treated with prednisolone and considered as treated group Prednisolone was given orally in a dose of 5 mg three times daily for three months. The semen analysis, SMI HOST and SPA were performed before and after treatment with prednisolone. The number of semen samples in the treated group was 144 and in the control fertile group was 80. HOST-SPA positive semen was exposed to antisperm antibodies separation (ASAS) and invitro sperm activation prior to intra-uterine insemination.
Results: The SMI was significantly higher in the post-treated group compared to pretreated group (240 vs. 52.5, P<0.0l). The SPI in the control group was significantly higher than the post and pre-treated groups. The HOST and viability test results were significantly increased in the post-treated group compared to pre-treated group (73.42 vs. 48.56 and 71.36 vs, 50. 74 respectively, P<0.01). The sperm penetration rate, sperm decondensation rate and sperm penetration index were significantly increased in the post-treated vs. pre-treated groups (26.49 vs. 10.84, 10.91 vs. 3.47, P<0.05, 14.45 vs. 4.30, P<0.01 respectively). HOST-SPA positive semen was used for intra-uterine insemination (IUI). The semen was exposed to ASAS and in vitro activation prior to IUI and resulted in 42.86% pregnancy rate per cycle. The pregnancy was confirmed by the observation of gestation sac and viable fetal hear beet 5 weeks following IUI.
Conclusion: It was concluded from the results of the present study that treatment of immunological infertile men with prednisolone improves SMI, sperm viability and SPA results. The application of HOST-SPA positive semen for IUI resulted in successful pregnancy. The authors indicate that these viable spermatozoa with high fertilizable potential could be used for IVF and/or ICSI in immunologically infertile men.
One hundred samples of root canal bacteria were isolated from patients teeth with primary and secondary infected root canal from all the ages . Biochemical and microscopial tests were done for identification of these isolates. Twenty four isolates were confirmed as E. faecalis species by using these tests. Genetic diagnosis for the all isolates was also done by using polymerase chain reaction ( PCR ). Thirty two isolates were confirmed to belong to E. faecalis species by using this test.
The accumulation of sediment in reservoirs poses a major challenge that impacts the storage capacity, quality of water, and efficiency of hydroelectric power generation systems. Geospatial methods, including Geographic Information Systems (GIS) and Remote Sensing (RS), were used to assess Dukan Reservoir sediment quantities. Satellite and reservoir water level data from 2010 to 2022 were used for sedimentation assessment. The satellite data was used to analyze the water spread area, employing the Normalized Difference Water Index (NDWI) and Modified Normalized Difference Water Index (MNDWI) to enhance the water surface in the satellite imagery of Dukan Reservoir. The cone formula was employed to calculate the live storag
... Show MoreThe complexity and partially defined nature of jet grouting make it hard to predict the performance of grouted piles. So the trials of cement injection at a location with similar soil properties as the erecting site are necessary to assess the performance of the grouted piles. Nevertheless, instead of executing trial-injected piles at the pilot site, which wastes money, time, and effort, the laboratory cement injection devices are essential alternatives for evaluating soil injection ability. This study assesses the performance of a low-pressure laboratory grouting device by improving loose sandy soil injected using binders formed of Silica Fume (SF) as a chemical admixture (10% of Ordinary Portland Cement OPC mass) to di
... Show MoreThe -mixing of - transition in Er 168 populated in Er)n,n(Er 168168 reaction is calculated in the present work by using a2- ratio method. This method has used in previou studies [4, 5, 6, 7] in case that the second transition is pure or for that transition which can be considered as pure only, but in one work we applied this method for two cases, in the first one for pure transition and in the 2nd one for non pure transitions. We take into accunt the experimental a2- coefficient for p revious works and -values for one transition only [1]. The results obtained are, in general, in agood agreement within associated errors, with those reported previously [1], the discrepancies that occur are due to inaccuracies existing
... Show MoreThe - M ultiple mixing ratios of -transitions from levels of 56Fe populated in 56 56 Fe n n Fe ( , ) reactions are calculated by using const. S.T.M. This method has been used in other works [3,7] but with pure transition or with transitions that can be considered as pure transitions، in our work we used This method for mixed - transitions in addition to pure - transitions. The experimental angular distribution coefficients a2 was used from previous works [1] in order to calculet - values. It is clear from the results that the - values are in good agreement or consistent, within associated errors, with those reported previously [1]. The discrepancies that occur are due to inaccuracies existing in the expe
... Show MoreSpectrophotometric method was developed for the determination of copper(II) ion. Synthesized (2,2[O-Tolidine-4,4-bis azo]bis[4,5-diphenyl imidazole]) (MBBAI) was used as chromogenic reagent at pH=5. Various factors affecting complex formation, such as, pH effect, reagent concentration, time effect and temperature effect, have been considered and studied. Under optimum conditions concentration ranged from (5.00-80.00) µg/mL of copper(II) obeyed Beer`s Low. Maximum absorption of the complex was 409nm with molar absorpitivity 0.127x104 L mol-1 cm-1. Limit of detection(LOD) and Limit of quantification were 1.924 and 6.42 μg/mL, respectively.
... Show MoreThis research investigates manganese (Mn) extraction from Electric Arc Furnace Steel Slag (EAFS) by using the Liquid-liquid extraction (LLE) method. The chemical analysis was done on the slag using X-ray fluorescence, X-ray diffraction, and atomic absorption spectroscopy. This work consisted of two parts: the first was an extensive study of the effect of variables that can affect the leaching process rate for Mn element from slag (reaction time, nitric acid concentration, solid to liquid ratio, and stirring speed), and the second part evaluates the extraction of Mn element from leached solution. The results showed the possibility of leaching 83.5 % of Mn element from the slag at a temperature of 25°C, nitric acid co
... Show MoreAl-Aziz's book was chosen as a subject for research as it is one of the important books in the Islamic world in general and the Shafi'i school in particular and its author Imam Abdul Karim bin Mohammed bin Abdul Karim Al-Rafi's, who in the doctrine of the jurisprudence has made prominent lines and left behind invaluable scientific treasures in the service of religion. Its importance is summarized as follows:
1. Being an explanation of the book (brief) of the argument of Islam Imam Ghazali (God's mercy), one of the five books adopted in Shafi'i jurisprudence.
2. His work is Imam Abu al-Qasim al-Rafii known for the brilliance of the investigation and the power of weighting in the doctrine.
3. It is considered an encyclopedia in Sh
A new Schiff base complex was prepeard and characterized: Chloro –Oxo (bis(Ohydroxy benzaldehyde) O-phenylene di imination ) Vanadium (V) with general formula (VOLCL). Complex was studied by using Three different organics Organic The photo chemistry of this solvent with different polarity . These solvents were ( Acetone,pyridinest chloro form) . It was found that the chelate Vanadium (V) complex decomposed photochemically in these solvents during . In the tra oxidation –reduction reaction leading to free radical derived in the ligand of shiff base ℓ .Vanadium IV chelate complex . It was also found that the quantum yield of photo decomposition (фd) and Activity ratio did not de
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