Background: Men with azoospermia require testicular and epididymal sperm aspiration for intracytoplasmic sperm injection. Women over 37 years of age demonstrate
an increase number of immature oocytes after induction of ovulation. The development of a technique for in vitro maturation (IVM) and fertilization (IVF) of
the oocytes using testicular, epididymal and vassal sperm (TS, ES, VS, respectively) will be of therapeutic value for the treatment of azoospermic patients.
Objectives: The goal of the study was to develop an animal model for the treatment of infertile couples with obstructive azoospermia and immature oocytes.
Materials and Methods: Canine ovaries and testes were collected from a local animal clinic. Immature oocytes were retrieved from the ovaries and cultured in modified tissue
culture medium (MTCM). The mature oocytes were in vitro fertilized by TS, ES, and VS. The normality of the fertilized oocytes was studied.
Results: Sperm motility index was significantly higher (P<0.01) in sperm retrieved from the vas deference compared to ES and TS. The concentration of the sperm was
significantly higher (P<0.05) in the testes compared to epididymis and vas deference while VS and ES normal morphology were significantly (p<0.01) better than TS. Viable oocyte
percentage was significantly higher (P<O.05) in 72 hours versus 96 hours culture durations. Significant higher IVF was reported in VS compared to other groups (P < 0.05) and the SPI
was significantly higher after 96 versus 72 hours duration (P<O.05).
Conclusion: IVM of the oocytes in MTCM for 72 and 96 h resulted in a significant increase in IVM rate compared to 48,120, and 144 h durations. In vitro viable mature
oocytes showed higher IVF rates when fertilized with VS compared to ES and TS. The results showed that canine oocytes are good model for azoospermic patients.
Background: There are many sources for Leptin secretion, and it is activated by binding with its receptor known as leptin receptor, that play a role in male infertility.
Objective: To assess the levels of leptin and leptin receptors in seminal plasma among primary infertile men and its impact on semen parameters.
Patients and Methods: A case control study of 75 primary infertile males and 40 healthy individuals who were enrolled in this study during March 2013 to May 2013. Estimation of age, body mass index (BMI), semen analysis, seminal plasma leptin, leptin receptor and testosterone hormone concentration were done for all study subjects.
Results: Highly significant difference found in mean of semen parameters of infertile male
In-situ gelation is a process of gel formation at the site of application, in which a drug product formulation that exists as a liquid has been transformed into a gel upon contact with body fluids. As a drug delivery agent, the in-situ gel has an advantage of providing sustained release of the drug agent. In-situ gelling liquid suppositories using poloxamer 188 (26-30% W/W) as a suppository base with 10% W/W naproxen were prepared, the gelation temperature of these preparations were measured and they were all above the physiological temperature. Additives such as polyvinylpyrrolidin "PVP" ,hydroxylpropylmethylcellulose "HPMC", sodium alginate and sodium chloride were used in concentration ranging from (0.25-1
... Show MoreThis study was designed to investigate the effects of licorice extract (Glycyrrhiza glabra L.) addition to semen diluters on ram sperm progressive motility during storage at 5 ˚C for 72 h. Semen was collected from 3 proven Awassi rams. Licorice extract powder was added at levels of 1, 5, 10, 50 and 100 µg per ml. of diluter. Diluter containing no licorice extracts served as control (0).Progressive motility was estimated subjectively after dilution (0h), and at 24, 48, and 72 h of storage. The experiment was replicated 2 times with egg yolk-tris (EYT) diluter and 2 times with yolk- glucose-citrate diluter. Progressive motility increased significantly (p < 0.01) in levels of licorice extract 1, 5, 10, 50 and 100 µg / ml
... Show MoreThe purpose of this work was to study the effects of the Nd:YAG laser on exposed dentinal
tubules of human extracted teeth using a scanning electron microscope (SEM). Eighty 2.5mm-thick
slices were cut at the cementoenamel junction from 20 extracted human teeth with an electric saw. A
diamond bur was used to remove the cementum layer to expose the dentinal tubules. Each slice was
sectioned into four equal quadrants and the specimens were randomly divided into four groups (A to D ).
Groups B to D were lased for 2 mins using an Nd:YAG laser at 6 pulses per second at energy outputs of
80 , 100 and 120 mJ. Group A served as control. Under SEM observation, nonlased specimens showed
numerous exposed dentinal tubules. SEM o
Measuring the efficiency of postgraduate and undergraduate programs is one of the essential elements in educational process. In this study, colleges of Baghdad University and data for the academic year (2011-2012) have been chosen to measure the relative efficiencies of postgraduate and undergraduate programs in terms of their inputs and outputs. A relevant method to conduct the analysis of this data is Data Envelopment Analysis (DEA). The effect of academic staff to the number of enrolled and alumni students to the postgraduate and undergraduate programs are the main focus of the study.
The study is designed to evaluate the effect of the aqueous extract of the P. lanceolata plant, as well as to know the effect of the drug CCl4 on the formation of micronucleus in vivo 48 female albino mice. In the study mice were separated into eight groups treated intraperitoneally for seven day first group Negative control, second positive control( CCl4 0.02%), third group aqueous extract (250 mg/kg), fourth group aqueous extract (500 mg/kg), fifth group (CCl4 0.02%) plus aqueous extract (250 mg/kg), sixth group (CCl4 0.02%) plus aqueous extract (500 mg/kg), seventh group aqueous extract (250 mg/kg) plus (CCl4 0.02%), and eighth group aqueous extract (500 mg/kg) plus (CCl4 0.02%). The genetic-cellular asp
... Show MorePeroxynitrite is one intermediate of reactive nitrogen species with bactericidal and cylotoxic effects. Fluoroquinolones. drugs used for salmonella infections, are interacted v\ith nitrogen species and their baeterieida effect is influenced by these species. This study aims to assess serum peroxynitrite level in patients with enteric (typhoid) fever and. to investigate the effect of ciprofloxacin or amoxicillin on serum peroxynitrite level as well as in aqueous buffer solution in vitro. Thirty patients with enteric fever diagnosed clinically and serologically and twenty healthy individuals served as controls were admitted in this study. None of our sample was received anli-salmonellosis agents. Our resu
... Show MoreBackground: To determine the DNA content in subfertile patients and to correlate it with seminal sperm concentration.
Design: Prospective observational study.
Setting: College of Medicine, Dept. of Physiological chemistry and Institute for embryo Research and infertility treatment-University of Baghdad.The study was conducted
through years 2004-2005.
Methods: A random sample of 61 subfertile male patients undergoing semen evaluation and aged from 20-45 years were studied. Semen samples were assessed for seminal sperm
concentration microscopically and were classified into 3 different groups according to count (million/ml).Then sperm D N A content (μg/ml) was estimated using a microchemical
spectropho
There are different types of young isolated NSs: radio pulsars, compact central X-ray sources in supernova, magentas: anomalous x-ray pulsars (AXPs) and soft gamma-ray repeaters (SGRs).This paper shows that the value of magnetic field (B), characteristic age ( ), spin down luminosity ( equilibrium period ( and Flux density ( ) was determined depending on some properties of pulsar star, such as the value of period of the pulsar (P) and the time derivative period ( for sample stars which were adopted. The model that which adopted is Hallo Cone Model. The results showed that the Normal pulsar stars have a big magnetic field, equilibrium period and Spin down than the Millisecond pulsar stars.But Millisecond pulsar stars have large values of
... Show MoreIron status can affect the outcome of