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Detection of Human Cytomegalovirus in Colorectal Adenocarcinoma by In Situ Hybridization Technique

Background: Human Cytomegalovirus (HCMV) infects a wide range of human cells, including colonic epithelial cells that give rise to adenomas and adenocarcinomas. Persistent productive infection of tumor cells is essential for oncomodulation by HCMV.This study aimed to detect HCMV matrix protein using in situ hybridization technique (ISH) in colorectal adenocarcinoma compared to normal colon tissues, and to the presence of cytomegalovirus inclusion bodies in infected colorectal carcinomas.
Patients and methods: Twenty six of colorectal adenocarcinomas were obtained in paraffin-blocks compared to 10 normal colon specimens which were age and sex matched as control group. Detection of HCMV was obtained by in situ hybridization technique.
Results:The biotinylated probe specific for DNA encoded HCMV-matrix protein showed hybridization with nucleic acid in 20 cases out of (26) of colorectal adenocarcinomas representing (76.9%) compared to normal colon tissue which revealed no hybridization signals. Moderate to high scoring signals were detected in moderate to poorly differentiated groups. Inclusion bodies were detected in 11 (42.3%) cases with positive hybridization signals.
Conclusion: HCMV may play a role in the colorectal adenocarcinoma pathogenesis. In situ hybridization test are considered the most sensitive and specific tools for detection of HCMV DNA in tissues. Epidemiological, histopathological identification of cytomegalic inclusion bodies and molecular studies are necessary to confirm the association of HCMV and colorectal tumorogenesis in Iraqi population.

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Publication Date
Mon Jul 20 2020
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Determined the Concentration Elements to Human Nail Using X- Ray Fluorescence Technique

Cancer constitutes a serious disease and a major health problem in worldwide, a lot of people were infected with this dangerous disease, Therefore, there must be attention to this disease through diagnosis and prevention there. In this study, we determined the relationship between the Cancer and the concentration of trace elements by comparing the concentration trace elements for infected and non-infected people. The trace elements concentrations to nails are one of the diagnostic criteria that easily to detect and dated this disease without any harm to the patient. Eight nails samples were collected from cancer-infected and eight samples from non-infected of the relatives of first-degree. All samples were measured by the concentrations

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Publication Date
Thu Mar 30 2017
Journal Name
Iraqi Journal Of Pharmaceutical Sciences ( P-issn 1683 - 3597 E-issn 2521 - 3512)
The In Situ Expression of IL-6 and IL-1? in breast cancer patients

Breast cancer is the second most common cancer in women world. Multiple Cytokines appear to have a dominant role in human breast cancer formation. Estimation of the in situ expression of  IL-6 and IL-1β in breast cancer patients. A sixty patients with breast cancer BC were divided into two clinical subgroups, (30) with malignant breast cancer MBC and (30) with benign breast tumor as a control group according to histological examination. In situ hybridization technique used for detection of IL-6 and IL-1β mRNA sequence in two groups.  The results showed that percentages of mRNA expression of IL-6 and IL-1β were in (≥ 11-50%) for malignant breast cancer. This research also investigated that (73.3%) of beni

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Publication Date
Sun Apr 03 2005
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Uses of CD31 monoclonal Antibody for the Assessment Of Angiogenesis as a prognostic Factor in Gastric Adenocarcinoma

Background &objectives: Angiogenesis plays an important role in the growth, progress and the metastasis of solid tumors. This study was done to determine the significance of angiogenesis as another prognostic factor in gastric carcinoma in an attempt to identify the patients at high risk of recurrences after surgical therapy who may benefit from postoperative adjuvant therapy.
In this study the correlation between the microvessel count(MVC) and the various clinicopathological factors was investigated. A highly significant statistical correlation was found between the mean microvessel count in different histological grades and the stage of gastric adenocarcinoma .
No correlation was found between the MVC and the age and sex of t

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Publication Date
Sun Jan 01 2023
Journal Name
Journal Of Advanced Pharmaceutical Technology & Research
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Publication Date
Fri Nov 24 2023
Journal Name
Iraqi Journal Of Science
Isolation and Identification of Fungal Propagation in Stored Maize and detection of aflatoxin B1 Using TLC and ELISA Technique

Aflatoxin B1 (AFB1) is a mycotoxin produced mainly by fungi Aspergillus flavus in food and animals feed. It is considered as a carcinogenic toxin for human and animals. The current study is designed to investigate the incidence of mycoflora in twenty four samples of local stored maize collected from Iraqi governorates; investigate the presence of aflatoxin B1 on these samples using TLC and ELISA techniques. The fungi recovered from maize samples were Aspergillus flavus (18.57 % ), Fusarium spp. (12.8 % ), A. ocraceus (9.96 % ) , A. terrus (9.07 % ), A. fumigatus (8.46 % ) , Alternaria spp. (6.40 % ) Rhizopus spp. (4.98 % ), A. niger spp., A. oryzae spp. (4.80 % ), Penicillium spp. (4.53 %) A. versicolor spp., Rhizoctonia spp. (4.27 %), A

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Publication Date
Sun Feb 13 2022
Journal Name
Iraqi Journal Of Science
In Situ Measuring of Relative Dielectric Permittivity to Calculate Electromagnetic Wave Velocity

The relative dielectric permittivity (dielectric constant) values for various materials are measured on site. The calculated values that are derived from measuring the ratio of the electrical capacitance of a cell when the sample of the material is placed. Parallel plate technique that consists of two parallel circular metallic plates is used. The instrument made up of aluminum which acts as electrodes and stuffed inside the PVC cylinder. Then the thin sheet of material (models) sandwiched between the two plates. Calculation of dielectric constant (εr) through this new technique gives a new velocity value of electromagnetic wave that propagates underground. These measured velocity values will utilize in Ground Penetration Radar (GPR) da

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Publication Date
Sun Dec 22 2019
Journal Name
Iraqi Journal Of Pharmaceutical Sciences ( P-issn 1683 - 3597 E-issn 2521 - 3512)
Formulation and Evaluation of Sustained Release Sumatriptan Mucoadhesive Intranasal in-Situ Gel

    

Sumatriptan(ST) is a selective agonist at serotonin 5-HTI receptors, as well as 5-HT1B/1D subtypes. It is effective for acute migraine attacks, but has a short half  life (about 2 hours) and low oral bioavailability (15%). The purpose of this study was to develop and optimize nasal mucoadhesive in-situ gel(IG) of ST to enhance nasal residence time for migraine management. Cold method was used to prepare different formulas of  ST nasal IG, using thermosensitive polymers (poloxamer 407  alone or with poloxamer 188) with a mucoadhesive polymer hyaluronic acid (HA) which were examined for gelation temperature and gelation time, pH, drug content, gel strength, spreadability, muc

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Publication Date
Wed Jan 18 2017
Journal Name
International Conference And Workshops On Basic And Applied Science
Identifying phenolic compounds in some genera belonging in the Amaranthaceae family by HPLC technique

Identifying phenolic compounds in some genera belonging in the Amaranthaceae family by HPLC technique

Publication Date
Sun Aug 06 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Measurement Of Organic Concentration in Aldehydes By The Derivative Neutron Activation Analysis Technique

The aim of this paper is determine the concentration of the organic oxygen in some organic compounds (Aldehydes ) by the derivative neutron activation analysis technique, and the derivative of the oxygen by the nitrogen equivalent toit and the irradiation of anew sample in flounce (1.73*106  n.cm-2.s-1) by the neutron generator .Then the calculation of the radioactivity which is done by using NaI(Tl) . After that we determine the concentration of nitrogen by calibration curve that includes nitrogen compounds which have apparent chemical and physical characteristics .For comparison the result is done by using keldal method.           

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Publication Date
Tue Jul 01 2014
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Detection of Cd69 Marker by Flow Cytometry in Iraqi Patients with Coeliac Disease.

Background: Coeliac disease is a chronic, immunologically determined form of enteropathy affecting the small intestine in genetically predisposed children and adults. It is precipitated by the ingestion of gluten-containing foods. It is also referred to as celiac sprue, gluten-sensitive enteropathy, or nontropical sprue. CD69 is a lymphoid activation antigen whose rapid expression makes it suitable for the detection of T-ce

J Fac Med Baghdad

2014; Vol.56, No .2

Received Feb .2014

Accepted Apr. 2014

 
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