Background: Molecular DNA hybridization has confirmed more than 120 different human papilloma virus (HPV) genotypes. A small group of them have high- risk oncogenic potential. Many studies have described an association of such high risk-HPV genotypes with a variety of oral lesions including squamous cell carcinoma, leukoplakia, and lichen planus.
Materials and Methods: A total number of 42 tissue specimens, representing 27 patients with oral lichen planus and 15 apparently-healthy oral tissues, were included in this study. The molecular methods for HPV detection and genotyping were performed by in situ hybridization(ISH) using cocktailed- and specific high- risk HPV DNA probes, respectively.
Results:The overall percentage of HPV in the total group of patients with oral lichen planus (OLP) was 33.3 %. Negative HPV DNA- ISH reactions were detected in all tissues of the control group. The overall genotyping results revealed HPV 16- DNA in all HPV- positive oral lichen planus tissues , while none of these OLP tissues showed ISH reactions for HPV 18-DNA or HPV 31/33- DNA.
Conclusions: The significant incidence of such high oncogenic HPV genotype in those patients with oral precancerous lesions could have a relevant importance along its pathogenesis and the multisteps oral carcinogenesis , HPV-16-associated oral lichen planus that has mostly previliged the site of cheek mucosa represents a herald indicator for spread of such sexually important transmitted infection among Iraqi general population.
Magnetic Resonance Imaging (MRI) is one of the most important diagnostic tool. There are many methods to segment the
tumor of human brain. One of these, the conventional method that uses pure image processing techniques that are not preferred because they need human interaction for accurate segmentation. But unsupervised methods do not require any human interference and can segment the brain with high precision. In this project, the unsupervised classification methods have been used in order to detect the tumor disease from MRI images. These metho
... Show MoreStool speeimens were collected from pati nts who presented for various medical c:omplaints in out patien.t Laboratories (The Central Health Laboratories / Baghdad,). Every ,$pecim-en wa:s examined by Conventional m jcroscopic exatninatitnl (CME) ·and te.§t d, by IgG ELISA kit.
Antibody against d1e Entamoeba histolylica, det cted by ELISA, has ·the potential to become a vah,mble adjunct to blood diagn9stics and make it more affective, .although there is no repJacement fo'e the
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... Show MoreAudio-visual detection and recognition system is thought to become the most promising methods for many applications includes surveillance, speech recognition, eavesdropping devices, intelligence operations, etc. In the recent field of human recognition, the majority of the research be- coming performed presently is focused on the reidentification of various body images taken by several cameras or its focuses on recognized audio-only. However, in some cases these traditional methods can- not be useful when used alone such as in indoor surveillance systems, that are installed close to the ceiling and capture images right from above in a downwards direction and in some cases people don't look straight the cameras or it cannot be added in some
... Show MoreCombining ultrasonic irradiation and the Fenton process as a sono-Fenton process, the chemical oxygen demand (COD) in refinery wastewater was successfully eliminated using response surface methodology (RSM) with central composite design (CCD). The impact of two main influential operational parameters (iron dosage and reaction time) on the COD removal from wastewater generated by an Iraqi petroleum refinery facility was explored. Removal of 85.81% was attained under the optimal conditions of 21 minutes and 0.289 mM of concentration. Additionally, the results revealed that the concentration of has the highest effect on the COD elimination, followed by reaction time. The high R2 value (96.40%) validated the strong fit of the mo
... Show MoreThe investment however "was its description and meaning, it remains a resident" in the composition of capital assets located in the forefront of the creation of productive assets, and this means that the investment in the productive sectors is a priority in achieving capital accumulation, on any other investment that takes place with the stages of advanced development of formation , not forgetting "to humans and investment humans as head of real money product, the source of the economic surplus and accumulation, and the source of producing values, and if human labor was the source of value, and the human was the source of work, therefore humanitarian work on different levels and skills presents capital", so the investment in huma
... Show MoreA total of 172 clinical were obtained over 6 months. Klebsiella spp. was detected in 58 (33.7%) samples with a high percentage 29 (50%) in urine in female and low percentage 1(1.7%) in pus and burn swabs in male, and the vaginal swab was 1(1.7%). The female to male ratio was 3.1:1. PCR detection showed that 51(87.93%) out of 58 produce 108 bp. product with rpoB specific primer that represented K. pneumonia. Whereas 7(12.07%) showed PCR product with 343 bp by K. oxytoca specific primer (peh X), furthermore, the sequences of two selected isolates showed that the species related to K. oxytoca strain CAV1335, and to K. oxytoca strain CAV1374. Five selected isolates were re-tested by the gyr A primer, all were showed specific band product wit
... Show MoreBackground: Helicobacter pylori are important gastrointestinal pathogen associated with gastritis, peptic ulcers, and an increased risk of gastric carcinoma. There are several popular methods for detection of H. pylori (invasive and non-invasive methods) each having its own advantages, disadvantages, and limitations, and by using PCR technique the ability to detect H. pylori in saliva samples offers a potential for an alternative test for detection of this microorganism. Materials and methods: The study sample consists of fifty participants of both genders, who undergo Oesophageo-gastrodudenoscopy at the Gastroenterology Department of Al-Kindy Teaching Hospital Baghdad/ Iraq, during five months period from January 2014 to May 2014. They we
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