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Immunostimulatory, Antibacterial and antibiofilm activity of purified Donkey colostrums lactoferrin on multidrug resistance Serratia liquefaciens producing Intl gene

Background: Modern methods of biofilm prevention depended on the utilize of normal foodstuffs can solve antibacterial and antibiofilm problems.
Objective: To purify donkey lactoferrin, and evaluate antibiotic resisted Serratia liquefaciens which producing Intl gene and investigate the inhibitory action of lactoferrin on biofilm and stimulate immune response.
Methods: lactoferrin extracted from donkey milk, and purified by ammonium sulfate and Sephadex chromatography. Antibacterial and antibiofilm activities of lactoferrin on Serratia liquefaciens were assayed, and effect of lactoferrin on the innate immune response of mice was determined.
Results: Lactoferrin contains 9.88% carbohydrates, 128 ppm iron, and molecular weight was 85kDa. It reduced S.liquifaciens in lung tissues of mice, and TNF-α and IL-6 cytokine levels decreasing by oral uptake of donkey milk.
Conclusion: All isolates resistance to Amoxicillin-clavulanic acid, and Intl gene was 160 bp. Lactoferrin concentration was about 2.77mg/ml. lactoferrin increase innate immune response from S. liquefaciens infection. These outcomes can be evidence for the way to original comprehensions regarding the use of Donkey colostrums lactoferrin as an oral adjuvant for a wide range of diseases.

 

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Publication Date
Thu Jan 01 2009
Journal Name
Journal Of Educational And Psychological Researches
العنف الاسري ضد الاطفال

اولاً : اهمية البحث والحاجة اليه :

تعد الطفولة مرحلة مهمة من مراحل حياة الانسان لانها ثروة مجتمعاتنا ومستقبلها وهي تمثل اللبنة الاولى في تركيب المجتمعات اذا ما صحلت صلح المجتمع وأذا ما فسدت فسد ذلك المجتمع ، لذا فأن تنمية القوى العقلية والبدنية والنفسية للطفل لمراحل نموه المختلفة هي مرحلة مهمة وخطيرة في الوقت ذاته لكي تسهم في بناء شخصيتهم في المستقبل واستثمار طاقاتهم من اجل تطوير وخدمة مجت

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Publication Date
Fri Mar 01 2024
Journal Name
Baghdad Science Journal
Copper Nanoparticles Synthesized in Biopolymer Matrix and Their Application in Antibacterial Activity

Copper is a cheaper alternative to various noble metals with a range of potential applications in the field of nanoscience and nanotechnology. However, copper nanoparticles have major limitations, which include rapid oxidation on exposure to air. Therefore, alternative pathways have been developed to synthesize metal nanoparticles in the presence of polymers and surfactants as stabilizers, and to form coatings on the surface of nanoparticles. These surfactants and polymeric ligands are made from petrochemicals which are non- renewable. As fossil resources are limited, finding renewable and biodegradable alternative is promising.The study aimed at preparing, characterizing and evaluating the antibacterial properties of copper nanoparticle

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Publication Date
Wed Oct 01 2014
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Prevalence and Antibacterial Resistance of Gram Negative Bacteria Causing Respiratory Tract Infection In Critically Ill Patients.

Background: Nosocomial respiratory infections in the intensive care is one of the challenging issues, competing with other major causes of morbidity and mortality, that’s why it needs to be studied thoroughly.

Objectives: To assess the prevalence of colonization of respiratory tract by Gram-negative rods (GNRs) for critically ill patients in Baghdad teaching  hospital, and to determine antimicrobial resistance of the isolated strains.

Patients and Methods: A cross sectional study conducted on critically patients at the ICU/Baghdad teaching hospital during the period from December 2012-june 2013. thirty four  adult patients on mechanical ventilator  with e

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Publication Date
Tue Oct 31 2023
Journal Name
College Of Islamic Sciences
Crimes against offspring In Sharia and law

Crimes are prohibited by law and God Almighty has forbidden them with punishment or punishment.
The punishment is the prescribed punishments that the law has determined in its place and stipulated in the Book of God or the Sunnah of His Prophet, may God bless him and grant him peace.
Ta’zir: These are the punishments that are left to the ruler to assess according to what he sees as preventing corruption on earth and preventing evil. Extrapolation has proven that all the provisions of Islamic Sharia include the interests of the people, and they are based on five matters: what is in it is preserving the religion, what is in it is preserving the soul, and what is in it is preserving the soul. It involves preserving the mind, preserv

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Publication Date
Sat Dec 31 2022
Journal Name
Iraqi Journal Of Market Research And Consumer Protection
THE ROLE OF EFFLUX PUMP FOR ANTIBIOTIC RESISTANCE IN BACTERIA: THE ROLE OF EFFLUX PUMP FOR ANTIBIOTIC RESISTANCE IN BACTERIA

ABSTRACT                

The multi-drug resistant efflux pump is a glycoprotein pump whose function is to push foreign substances. The efflux pump is found in humans, animals. It also has wide-ranging properties in  bacteria and fungi. They are found in all species of bacteria, and efflux pump genes can be found in bacterial chromosomes or mobile genetic elements, such as plasmids. The most sensitive function that leads to a global problem is its resistance to antibiotics in bacterial cells, which increases the ability to bacteria from becoming strong virulence factors that most or all antibiotics cannot kill. It also has othe

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Publication Date
Sat Dec 01 2018
Journal Name
Al-khwarizmi Engineering Journal
Classical and Statistical Optimization of Medium Composition for Promoting Prodigiosin Produced by Local Isolate of Serratia Marcescens

Prodigiosin is a ‘natural red pigment produced by Serratia marcescens which exhibits immunosuppressive and anticancer properties in addition to antimicrobial activities. This work presents an attempt to maximize the production of prodigiosin by two different strategies: one factor at time (OFAT) and statistical optimization. The result of OFAT revealed that sucrose and peptone were the best carbon and nitrogen sources for pigment production with concentration of prodigiosin of about 135 mg/ L. This value was increased to 331.6mg/ L with an optimized ratio of C/N (60:40) and reached 356.8 with pH 6 and 2% inoculum size at end of classical optimization. Statistical experimental design based on Response surface methodology was co

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Publication Date
Sun Sep 01 2019
Journal Name
Baghdad Science Journal
Detection a New Antiseptic Resistant Variant of qac Gene in Some Multi Drug Resistant Staphylococcus aureus Isolated from Different Clinical Sources

The increasing use of antiseptic compounds creates selective pressure cause emergence of antiseptic resistance among Staphylococcus aureus .Resistance mechanism of antiseptic is driven mainly by multi drug resistant (MDR) efflux protein.Sixty five isolates of S.aureuswere collected from different clinical sources and subjected to 11 antibiotics most of them are recognized by efflux systems as extruded substrates. Range of efflux activity was estimated using cartwheel method. Simultaneous discrimination of antiseptic coding genes (qacA/B, smr and norA)as well as nuc and mecA genes among multidrug resistantS.aureus(MRSA) isolates was preformed using multiplex PCR assay

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Publication Date
Fri Dec 01 2023
Journal Name
Baghdad Science Journal
Gene Expression and Methylation Levels of PCSK9 Gene in Iraqi Patients with Coronary Artery Disease

The expression of the Proprotein Convertase Subtilisin/Kexin Type 9 gene (PCSK9) is inextricably related to lipid levels and a risk of atherosclerotic coronary artery disease (ASCAD). The present study aims to measure the quantity of PCSK9 gene expression and the effect of methylation on its expression level taking part in the pathogenesis of acute coronary artery disorder.

A current study included 150 subjects from the Iraqi population, 100 ASCAD patients and 50 healthy controls. The concentration of PCSK9 in each serum sample was determined by the ELISA technique, the expression levels of the PCSK9 gene in whole blood were estimated by RT-qPCR – Quantitative Reverse Transcription PCR method, and DNA

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Publication Date
Tue Feb 28 2023
Journal Name
Iraqi Journal Of Science
Biosynthesis of Nio Nanoparticles Using Prodigiosin Pigment and its Evaluate of Antibacterial Activity Against Biofilm Producing MDR- Pseudomonas Aeruginosa

       The entire investigation's focus was on the production of nickel oxide nanoparticles (NiONPs), using prodigiosin pigments produced by Serratia marcescens as a stabilizing and reducing agent. Nickel oxide nanoparticles are synthesized using nickel sulfate NiSO4 (10mg) with a concentration of prodigiosin (10g/100ml). Biosynthesized NiO nanoparticles have been characterized by using many techniques, such as (UV-Vis, AFM, XRD, FTIR, and FE-SEM). The AFM analysis revealed that NiONPs have an average diameter size of (41.77 mm), and the FE-SEM Image displays Spherical. Additionally, the effect of NiONPs with different concentrations on the bacteria Pseudomonas aeruginosa was measured and the inhibition

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Publication Date
Thu Oct 01 2020
Journal Name
Iraqi Journal Of Biotechnology,
Isolation and Identification of Multidrug Resistance Among Clinical and Environmental Pseudomonas aeruginosa Isolates

Pseudomonas aeruginosa is the most common opportunistic pathogen causing morbidity and mortality in hospitalized patients due to its multiple resistance mechanisms. Therefore, as a therapeutic option becomes restricted, the search for a new agent is a preference. So P. aeruginosa is an extremely versatile Gram-negative bacterium capable of thriving in a broad spectrum of environments, and this performs main problems to workers in the field of health. One hundred and fifty samples were collected from different sources from Baghdad hospitals, divided into two main groups: clinical (100) specimens and (50) samples as an environmental, collected from October 2019 to the March 2020. All of these samples were cultured by specific and differential

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