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The Relationship between Phylogenic Typing and Antimicrobial Susceptibility Patterns forEscherichia coliIsolatedfrom UTIs atMany Hospitals in Baghdad City
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Objective:The current study aime to isolate Escherichia colifrom urinary tract infections(UTIs) in many Baghdad hospitals. The study concentrate on phylogenic groups and this was done based on triplex PCRmethod by primers besieged to three genetic markers, chuA, yjaA and TspE4.C2. Evaluate the relationship of phylogenic groups of E. coli isolates with the antibiotic-non sensitive patterns. Methodology:Four hundredof E.coli bacteria isolated from urine samples from five hospitals in Baghdad city include: Ghazi AL-Hariri, Ibin- Al-Beledi , AL-Iskan , AL-Nooman and AL-Yarmoke hospitals. Phylogenetic categorization of E. coli isolates was completed by by means ofearlier reported triplex PCR-based on phylotypingprocedureusing primers besieged at three markers, chuA, yjaAand TspE4.C2. Phylogenetic combination was done on the starting point of the existence or nonexistence of the3 DNA fragments as follows: chuA–, TspE4.C2–, group A; chuA–, yjaA–, TspE4.C2+, group B1; chuA+, yjaA+, group B2; chuA+, yjaA–, group D. Because two possible profiles can be obtained for the groups A, B2, and D. Fifteen antibiotics second-hand for all foremost groups and their individual generations were used in this paper adjacent to all bacterial isolates. Results: The isolates ofE.colifrom UTI were distributed within thephylogroups B2 (43%);phylogroups D (28.25%);phylogroups A (24%) and B1 (3.5%), so phylogenic group B1 was prevalent among male patients of AL-Yermouk hospital ( 14%), followed AL-Iskan (3.75)% so, isolation rate of E.coli were higher among age (11-20 ) years (31.75 %), also(11%) were sensitivity to antibiotics, whilst (75.25%) were classified as extensive drug resistance pathogens, but (13.75% )were multidrug resistance and in Karkh areas, the frequency of phylogeneticB2 was (59.88%), followed phylogenetic A and D were ( 68.75 and 48.78%) respectively as compared to Rusafa areas were prevalence phylogenetic B2(40.12%),followedphylogenetic D and A were (51.22 and 31.25 %) , as well as most of these phylogenetic groups occurred more frequently in both groups B2 or D (43, 28.25%) respectively, also ( 2.75, 2.5% ) of phylogenic A were antibiotics sensitivity in both AL-Iskan hospital and Ghazi AL-Hariri hospital respectively whilst these phylogenic was multidrug resistance as percentage (9, 3.75) % respectively in both AL-Yermouk hospital and AL-Iskan hospital, Whilst in these hospital this phylogenic A was founded with extensive drug resistance (1, 0.75%) respectively, also most of phylogenetic group B2 occurred more frequently in AL-Yermouk hospital, so the sensitivity; multidrug resistance and extensive drug resistance as (2.25, 15 and 3.5%) respectively, also all phylogenetic groups E. coli isolates from UTI patients of five hospital weregrouped in two or three major group as A , B group contain major sub groups . Recommendations: The study recommends additional studies of the correlation between the Phylogenic group with antibiotic-resistance pattern for other bacterial types isolated from UTIs or the same bacteria isolated from other types of infections. From this study,it is recommendedto study onKlebsillaspp isolated from the infected patients urinewho submitted to the same hospitals orin the other provinces of the country.

 
 

 

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Publication Date
Tue Jul 17 2012
Journal Name
مجلة مركز بحوث التقنيات الاحيائية
Effecienty of Immobilization technique of Bacillus Sublilis Asperginase producer
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Abstract Twenty Bacillus isolated were obtained from different sample food and water. Bacillus B1 isolated was the highest asparaginase producer, it was identified as a strain of B. subtilis. The highest production of asparaginase was observed when mineral salt medium containing 0.3% asparagen, pH 8 and incubated at 40°c for 24 hrs. B. subtilis B1 cells were immobilized by entrapment methods (calcium alginate and agar), and by adsorption on solid surface such as sawdust and cotton. The result showed that the immobilized cells by adsorption on sawdust was the best, the immobilized cell retained 88% of asparginase activity after 48h while free cell retained 65%. Cells immobilized by adsorption on sawdust was incubated at different temperatu

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Publication Date
Tue Jan 15 2019
Journal Name
Arab Science Heritage Journal
استعمال إدرار الجمال والخراف والأبقار كعامل مضاد لبكتريا pseudomonas aeruginosa
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جمعت عينات من إدرار الجمال والخراف والأبقار واستعملت كعوامل مضادة للبكتريا باستعمال طريقة كأس الغراء وباستعمال 20 عزلة من عزلات بكتريا Pseudomonas aeruginosa . أجريت محاولات مختلفة لأجراء هذا الاختبار لغرض أيجاد أفضل تحضير لإدرار الجمال والخراف والأبقار يعطي قدرة أعلى على تثبيط نمو البكتريا مقارنة مع البنسلين بدءأ من الإدرار غير مخفف المرشح ثم بالإدرار المخفف . اظهرت الن

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Publication Date
Mon Mar 01 2010
Journal Name
Al-khwarizmi Engineering Journal
Heat Transfer of Single and Binary Systems in Pool Boiling
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The present research focuses on the study of the effect of mass transfer resistance on the rate of heat transfer in pool boiling. The nucleate pool boiling heat transfer coefficients for binary mixtures (ethanol-n-butanol, acetone-n-butanol, acetone-ethanol, hexane-benzene, hexane-heptane, and methanol-water) were measured at different concentrations of the more volatile components. The systems chosen covered a wide range of mixture behaviors.

The experimental set up for the present investigation includes electric heating element submerged in the test liquid mounted vertically. Thermocouple and a digital indictor measured the temperature of the heater surface. The actual heat transfer rate being obtained by multiplying the voltme

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Publication Date
Mon Mar 23 2020
Journal Name
Journal Of Engineering
Performance Analyses of 15 kW Grid-Tied Photo Voltaic Solar System Type under Baghdad city climate
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The performance analyses of 15 kWp (kW peak) Grid -Tied solar PV system (that considered first of its type) implemented at the Training and Energy Research Center Subsidiary of Iraqi Ministry of Electricity in Baghdad city has been achieved. The system consists of 72 modules arranged in 6 strings were each string contains 12 modules connected in series to increase the voltage output while these strings connected in parallel to increase the current output. According to the observed duration, the reference daily yields, array daily yields and final daily yields of this system were (5.9, 4.56, 4.4) kWh/kWp/day respectively. The energy yield was 1585 kWh/kWp/year while the annual total solar irradiation received by solar array system was 198

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Publication Date
Thu Jun 01 2017
Journal Name
Journal Of Economics And Administrative Sciences
Efficiency of public investment spending and its impact on economic growth in Iraq For the period 2003- 2013
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Occupy public investment spending, a great deal of attention since the thirties of the last century, to play important role in economic and social development process and therefore most of the countries are trying different degree of economic development to the completion of the largest amount of public investment, especially in infrastructure, which is one of the pillars essential to economic development, and in order to maximize utilization of the completion of public investment should focus on achieving efficiency in completion.
    The current reality of public projects in Iraq, indicating the absence of several key characteristics that must be provided if these proje

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Publication Date
Sun Jun 30 2019
Journal Name
Journal Of The College Of Education For Women
Qualities of Life Partner from the Perspective of a Sample of University Students
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The study aims at identifying the morphological and psycho-socio-economic qualities wished to be in a life partner among a sample of Palestinian youth. The total sample was (231) and consisted of  (83) male and (148) female students. Each student presented a detailed report on the qualities he/she wished to be in life partner. The study used the descriptive approach and content analysis method. The validity and stability of the analysis were calculated. The results showed eight qualities in both groups: physical, psychological, emotional, social, intellectual, familial, economic and academic. Female students were found to have more variations than male students in terms of the qualities preferable in the life partner. Male students

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Publication Date
Sun Feb 28 2021
Journal Name
Journal Of Economics And Administrative Sciences
The role of corporate merger in achieving synergy (Analytical research in a sample of companies of the Iraqi Ministry of Industry and Minerals)
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The research aims to shed light on the recent experience in the Iraqi business environment, which is the experience of the merger. To evaluate a recent experience in an important sector of the Iraqi business sectors, namely the industrial sector to enable decision-makers to review that experience to judge the extent of its success and address some of the lapses that experience that by measuring synergies can be judged on the success of the merger experience or not. The research community is the governmental industrial sector. The research sample included six cases of merger (14) companies before the merger. The Holt method was used to predict the net sales and total cost values ​​before the merger as if it were not merged. Th

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Publication Date
Wed Sep 27 2023
Journal Name
Iraqi Journal Of Pharmaceutical Sciences ( P-issn 1683 - 3597 E-issn 2521 - 3512)
Genetic Polymorphism in TNF-α Promoter Region: Its Association with Severity and Susceptibility to Rheumatoid Arthritis in Iraqi Patients with Active Disease
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Objectives: To study the prevalence of rs1799964 (-1031 T/C) and rs361525 (- 238 G/A) SNPs and their effect on the disease activity, severity, and cytokines production in newly diagnosed Iraqi rheumatoid arthritis patients. Patients and Methods: sixty-three patients were diagnosed by a specialist physician while attending the rheumatology unit and twenty control participated. The inflammatory markers were measured and PCR amplification and sequencing were performed to demonstrate TNF-α SNPs. Results: Regarding (-1031 C/T) SNP, the TT genotype and allele C were significantly present in the controls, and the CT genotype was distributed significantly in the patients. The TT genotype was mostly distributed in the mild-moder

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Publication Date
Thu Sep 01 2011
Journal Name
Journal Of Accounting And Financial Studies ( Jafs )
The Role of higher Education Institutes in restricting Administrative and Financial Corruption: An Analytic Reading
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Corruption, in all its categories and forms, is regarded as the nowadays virus which has greatly spread in most institutes and society, a matter that cause a great waste of resources.

      According to the reports of international transparency Institute, Iraq is regarded as one of the greatest countries in corruption.

      Regardless of the reasons and forms of corruption, the retreat in work – values and ethics are the main reasons behind that.

      Being the main source of providing qualified staff "educators" for the working market, the high education institutes face great challenges in standing against corruption inside and outside

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Publication Date
Mon Dec 30 2019
Journal Name
College Of Islamic Sciences
Among the hadiths of righteous deeds mentioned in the expiation of sins -An analytical study-
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The almighty Al-Bari imposed on Muslims easy and accessible worshipers for every individual who is eligible to commission, so whoever performed it rightfully, God rewarded it with the best reward. From the Muslim, the Almighty made the Almighty to the types of acts of worship and acts of righteousness as a reason for the remission of sins that were advanced and late, so I chose a topic (from the hadiths of righteous deeds mentioned in the atonement of sins) as a research drawn from the master’s thesis called (the hadiths of the atoners of sins that were presented and delayed - An analytical study- ), And collect in this research kinds of acts of righteousness that bring the mercy of God Almighty to His servants and be a reason for the

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