Objectives: Determine the age and gender distribution of children who experience diabetes mellitus (DM) under
the age of 15 years and the presence of some associated factors that might be a predisposing factor for the
disease including obesity.
Methodology: A cross-sectional study was conducted at diabetic clinic in Children Welfare Teaching Hospital
in Baghdad City during 2006. The study sample included diabetic children less than 15 years of age. Data were
taken from the patients' record and by direct interview with the patients' parents. Information included
demographic data, as well as past history of the patient and his/her family relative to diabetes and other immune
diseases.
Results: Data analysis showed that there was an equal distribution of patients among the three age groups (I-5,
6-10-, and 11-15 years) in a rate of 33% for each. Females had higher incidence rate (660/o) than males. Onset of
the disease was mostly at age group I-3 years (32%) followed by age group 4-6 years (30%). IIistory of viral
illness was present in 24% of patients and a positive family history of DM was found in 23% of them, and a
family history of autoimmune disease was positive in 13% of cases. Body weight at the first visit to the clinic
was mainly below the 50th percentile (42% within the 25th percentile) and only 4% of patients had a body
weight within the 75th percentile.
Recommendations: Health education about the disease and provision of health centers those are easily
accessible for early diagnosis and treatment and uninerrupted insulin supply.
High performance liquid chromatography was applied for the separation and identification of four antharquinone derivatives, aloe emodin, emodin, chrysophanol and physcion. The separation was carried out using Eurospher 100, C18 column (4.6 mm i.d. x 250 mm, 5 µm) under the following conditions: acetonitile (solvent A) and water: acetic acid (99.9: 0.1 v/v, pH 3.5)( solvent B) as a mobile phase with isocratic elution with 30% solvent B at flow rate 0.8 ml/min. The detection wavelength was set at 254 nm. The four antharquinone derivatives were isolated from the Iraqi rhubarb, Rheum ribes root by preparative TLC, their structures were identified by 1H NMR and used as standards for HPLC analysis. The percentages of alo
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