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Optimize and Purification of Keratinase Produced from Local Aspergillus Terreus A13 Isolate Using A Feather as Substrate
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     Keratin is a fibrous, insoluble structural protein that is highly cross-linked with hydrophobic, hydrogen, and disulfide bonds. Keratinases are enzymes that belong to the category of serine hydrolases that are capable of breaking down keratin. The results of the determination of the better fermentation system showed that the production of keratinase from local A.terreus A13 isolate by submerged fermentation (SmF) system was the best system to give the highest specific activity (113.4 U/mg) of keratinase compared with solid-state fermentation (SSF). The optimum conditions for keratinase production by SmF, were determined via cultivation conditions, including carbon source, nitrogen source, temperature, pH of the medium, and time of incubation were optimized to enhance the production of total keratinase production in a culture of A.terreus A13 with incubator shaker. The highest product of total keratinase was achieved in feather broth with 2 % sucrose, and 0.5 % soya bean, with a pH of 5.5 at 28 °C for 8 days. Separation and purification of keratinase from a local isolate of A.terreus A13 was done by precipitating with 0-75 % saturated ammonium sulfate, then by ion-exchange chromatography on DEAE-Cellulose column and sephadex G-150 gel. Partially purified keratinase gave an activity of 5.1 U/ml, protein concentration of 0.004 mg/ml, and specific activity of 1275 U/mg with purification fold of 4.96 and 49 % as yield. The aim of the present study was to optimize the production of keratinase from A. terreus A13, cultivated using optimum conditions, and its use for the biodegradation of feathers.

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Publication Date
Thu Aug 30 2018
Journal Name
Iraqi Journal Of Science
Using local Iraqi Bacillus isolate to reduce water salinity
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Microbial desalination cell (MDC) has been created for expelling water saltiness, power generation, and wastewater administration. The MDC comprised of three chambers (anode, center desalination, and cathode).Were tested ability of type locally isolated bacteria Bacillus spp.in produce electricity to water desalination. In recent study results showed that a remove where the salinity recorded 4000 ppm at room temperature at the voltages of 0.6 volts and less salinity at room temperature at 0.2 volts was 200 ppm. Recent results highlight the need to reduce time for reduce salinity decreased from 3500 ppm to 500 ppm the eleventh day at a voltage of 0.5 volts that depended on type of substrate.

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Publication Date
Sun Mar 07 2010
Journal Name
Baghdad Science Journal
Improvement of thermostable productivity ?-amylase from local isolate Bacillus licheniformis H14.
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(28)Bacterial local isolates of Bacillus sp. were obtained from soil samples. Isolates were tested for thermostable alpha- amylase production on solid media; fifteen isolates were able to develop clear zone around the bacterial growth after floating the plates with iodine reagent (Lugol's solution). There were further tested in submerged culture which led to selection of Bacillus sp. H14since it was the most efficient .Microbial and biochemical tests showed that the local isolate Bacillus sp.H14was refered to the species B.licheniformis that signed as H14 was refered to the species B.licheniformis H14 .,To get ahigher yield of alpha – amylase(48.70unit/mg protein) production from the local isolate B.licheniformis H14 . This study used

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Publication Date
Thu Dec 19 2024
Journal Name
Iraqi Journal Of Agricultural Sciences
ISOLATION, SCREENING AND PRODUCTION OF PHYTATE DEGRADING ENZYME (PHYTASE) FROM LOCAL FUNGI ISOLATE
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Publication Date
Fri Jan 26 2024
Journal Name
Iraqi Journal Of Science
The Extraction and Partial Purification of Wedelolactone from Local Eclipta alba Plant
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Abstract:
Eclipta alba is a weed growing in damp, moist puddles distributed in the tropical and subtropical regions, so the most weight of the plant is water, which reached in to 90%. The extraction method by using different solvents (Methanol, Ethanol, Hexane and Aqueous) showed, the best yield with methanol reached to 76%, and the yield decreased with ethanol and hexane reached 55% and 53% respectively, while the minimum yield observed with aqueous hot and cold extraction reached 11% and 5% respectively. The phytochemical compound characterization showed the compounds (Coumarines , Flavones ,Volatile Oil ,Tannins , Saponines , Glycosides ,Carbohydrates ,Alkaloids , Resins) with different percentage. The thin layer chromatography det

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Publication Date
Sun Jul 29 2018
Journal Name
Iraqi Journal Of Science
Determination of vasicine alkaloid efficacy as inhibitor to the activity of protease produced by a clinical isolate of Pseudomonas aeruginosa
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In the present study, the effect of vasicine alkaloid separated from Adhatoda vasica as an inhibitor agent on the activity of proteases enzyme isolated from Pseudomonas aeruginosa was investigated. forty isolates of Pseudomonas aeruginosa were collected from local hospital in Baghdad and then their ability for producing proteases was screened using quantification and semi- quantitative methods. Pseudomonas aeruginosa P1 was selected as the highest protease producer, which next identified as P. aeruginosa. It was found that the optimum culture conditions for protease production in submerged culture was in the tryptic - soya broth medium at 37° C with pH 8 for 48 hours. In addition, the study i

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Publication Date
Sat Aug 01 2020
Journal Name
Iop Conference Series: Materials Science And Engineering
Isolation and Identification of Local Bactria Produced from Soil-Borne Urease
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Abstract<p>The hydrolysis of urea by the enzyme urease is significant for increasing the irroles in human pathogenicity, biocementation, soil fertilizer, and subsequently in soil improvement. This study devoted to the isolation of urease from urea-rich soil samples collected from seven different locations. Isolation of the various bacterial species was conducted using nutrient agar. The identity of isolated urease was based on morphological characteristics and standard microbiological and biochemical procedures. The urease producing strains of bacteria were obtained using the urease hydrolysis test. The bacterial isolates produced from soil samples collected from different environments and treat</p> ... Show More
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Publication Date
Thu Mar 30 2023
Journal Name
Iraqi Journal Of Science
Production, Purification and Characterization of Uricase Produced by Pseudomonas aeruginosa
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In this study, detection of uricase production from Pseudomonas aeruginosa
isolates was done by applying colorimetric method, Uricase was purified from the
most potent isolate by precipitation using ammonium sulphate (80% saturation) then
purification was achieved using DEAE –Cellulose ion exchange and Sepharose 6B
gel filtration chromatography column, 16.4% of total enzyme was recovered with
specific activity 2337.5U/mg and 22.21folds of purification. Characterization of
uricase involved detection of optimal conditions for uricase activity, the maximal
activity was obtained at temperature 45ºC,while uricase appeared to be stable at
40ºC. Uricase showed optimal activity at pH 9 while pH stability was in the

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Publication Date
Tue Jan 31 2017
Journal Name
Journal Of Engineering
Studying the Adsorption of Lead from aqueous Solution using Local Adsorbent Material Produced from Waste Tires by Pyrolysis
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In this research a local adsorbent was prepared from waste tires using two-step pyrolysis method. In the carbonization process, nitrogen gas flow rate was 0.2L/min at carbonization temperature of 500ºC for 1h. The char products were then preceded to the activation process at 850°C under carbon dioxide (CO2) activation flow rate of 0.6L/min for 3h. The activation method produced local adsorbent material with a surface area and total pore volume as high as 118.59m2 /g and 0.1467cm3/g, respectively. The produced . local adsorbent (activated carbon) was used for adsorption of lead from aqueous solution. The continuous fixed bed column experiments were conducted. The adsorption capacity performance of prepared activated carbons in this work

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Publication Date
Thu Jan 21 2010
Journal Name
Iraqi Journal Of Veterinary Medicine
Production and Partial Purification of Heat-Stable Enterotoxin (A) Produced by Enterotoxigenic Escherichia coli
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A total of (25) stool samples were collected from children and adults (2- 4) years old suffering from diarrhea to isolate E. coli strains that produce heat-stable enterotoxin a (STa), and after performing microscopic examination, cultural characterization and biochemical identification only (11) isolates showed positive E. coli. STa activity was estimated by using suckling mouse assay (SMA) and from these (11) isolates only (5) showed STa activity and the one with the highest STa activity was selected for large scale production of STa, which was followed by partial purification using ion-exchange chromatography (normal phase) using DEAE sephadex A-50 column. After purification and determination of protein concentration by using the standard

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Publication Date
Wed Feb 08 2023
Journal Name
Iraqi Journal Of Science
Decolorization of Reactive Dyes by Spore-bound Laccase from Local Isolate of Bacillus sp.
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Utilization of bacterial activity for decolorization of coloured products is one the most promising industrial strategy, as an eco-sustainable and cost-competitive alter-native to physicochemical methods. Laccase production from Bacillus sp. was stud-ied for its decolorization influences on different dyes (Indian ink, Brilliant green, Bromothymol blue, Crystal violet, Safranin, Bromophenol blue, Methelen blue, Giemsa stain, Nigrosin, Toluidin blue, Neutral red, Phenol red, Hanna, Blood, Ben-gal rose B, Bromkresol green, 4-Bromoaniline, Aniline blue, 2,6-Dichlorophenol in-vophenol, Curcumin, Acridine orange, Indigo carmine, Xylene cynol FF,10G, Aliza-rine yellow GG and Kongorose). After 5 days of incubation of the spore-bound lac-case wit

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