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Increasing Amounts of Secondary Metabolites and Medicinal Compounds in Callus Culture of Moringa Oleifera (Lam.) Using Abiotic Elicitors

Moringa oleifera Lam. tree has often been considered a medicinal and economical plant for its nutritional and medicinal properties. This study is intended to use plant tissue culture and elicitation technologies to increase secondary metabolites in the callus cultures of the M. oleifera. Young nodal segments were cultured on Murashige and Skoog (MS) medium supplemented with different concentrations of plant growth regulators (PGRs). A combination of 1.5 mg/l NAA plus 0.5 mg/l TDZ resulted in a high callusing rate (70%) with the largest size. Various concentrations of Salicylic acid (SA) and Methyl jasmonate (MeJa) were individually added to the growth medium to evaluate their impact on the biomass of callus cultures, DPPH antioxidant activity, and accumulation of some flavonoids. Results indicated that all elicitors doses reduced weights and growth index of callus. While DPPH assay recorded high significant values at high concentrations of SA and MeJa compared to other treatments. HPLC analysis revealed that every concentration of SA and MeJa used led to rising flavonoids content, including Baicalin, Rutin, Hesperidin, Rosmarinic acid, Quercitrin, Quercetin, and Kaempferol. It should be noted that 20 mg/l MeJa gave the highest flavonoid content compared to the control and other treatments.  In conclusion, the in vitro elicitation by SA or MeJa can improve beneficial biological substances like flavonoids and other antioxidant compounds in Moringa callus cultures.

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Publication Date
Tue Nov 19 2024
Journal Name
Journal Of Physical Education
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Publication Date
Mon Sep 28 2020
Journal Name
Journal Of Physical Education
The Effect of Increasing Rehabilitation Program Using Electric Stimulation On Rehabilitating Knee Joint Working Muscles Due to ACL Tear In Athletes

The research aimed at designing a rehabilitation program using electric stimulation for rehabilitating knee joint working muscles as a result of ACL tear using an apparatus developed by the researchers that stimulate the muscle vibration and work as well as the ability to rehabilitate the join in shorter periods. In addition to that, it aimed at identifying the effect of this program on rehabilitating the knee joint working muscles. The researchers used the experimental method on Baghdad clubs’ players who suffer from complete knee joint ACL tear aged (19 – 24) years old. The results showed that the training program developed the working muscles significantly achieving normal levels of activity.

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Publication Date
Sun Jan 30 2022
Journal Name
Iraqi Journal Of Science
Estimation of Vitamin C and the Fructose Levels in Some Medicinal Plants and their Effects on Iron Bioavailability in Rats

      Iron deficiency is considered as a common problem facing the general world population. In the current research, experiments were conducted to evaluate the effects of aqueous extracts of Acacia senegal (Gum Arabic, GA), Spinacia oleracea (spinach), Zea mays (corn), and Capsicum annuum (red chili pepper) on iron and ferritin levels in rats. Vitamin C and fructose levels were first estimated in these plants by using High-Performance Liquid Chromatography (HPLC). The results showed that the GA extract contained the highest level of fructose (853 mg.L-1), followed by red chili pepper (635 mg.L-1), corn (521 mg.L-1), and spinach (271 mg.L-1

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Publication Date
Mon Mar 08 2021
Journal Name
Baghdad Science Journal
Mixtures of beeswax and paraffin their importance in bee culture and their role

Proved the validity of all mixtures or not to accept the bees and then resistant infection worm wax Minor has accepted honey bee colonies all mixtures beeswax with paraffin wax industrial all ratios used made ??of a mixture to DVD waxy full and used naturally in breeding Houdnth In store honey and pollen evendiversified Bravian and natural wax

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Publication Date
Thu Dec 01 2016
Journal Name
Iraqi Journal Of Science
Callus Induction and Proliferation as Affected by EMS and UV-B Mutagens in Rice (Oryza sativa L.) Genotypes

Seeds of the two rice genotypes namely Amber 33 (A33) and Amber Baghdad (AB) were divided into two groups; the first was presoaked in different concentrations of ethyl methane sulphonate (EMS) as chemical mutagen for different duration times (3, 6 and 12) hrs, and the other was exposed to different exposure times of ultra violate (UV-B) radiation (280-320 nm) as physical mutagen for different times (20, 40 and 60) min at room temperature. Treated and non-treated seeds were transferred into the callus induction medium containing 2.5 mg/L 2,4- dichlorophenoxy acetic acid (2,4-D) and 0.5 mg/L benzyl adenine (BA) under aseptic conditions. Calli were divided into two groups the first was treated with several EMS concentrations (0.0, 0.50. 1.0, 1

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Publication Date
Mon Mar 08 2021
Journal Name
Baghdad Science Journal
Manufacturing concentrates Alholucramih for use in increasing the efficiency of solar cell

A new way to Systems concentrates have been clarified and that allows a concentration high and analysis to automatically wavelengths of the spectrum of this system analyst of the spectrum and the center is built on Holucram Nafez gives less absorbency with efficient diffraction high when the wavelength (900 nm), which will be useful for Khallaya solar

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Publication Date
Wed Mar 10 2021
Journal Name
Baghdad Science Journal
Effect of Gamma Radiation on Callus Induction and Plant Regeneration of four Wheats Triticum aestivum L. Genotypes

The effect of gamma radiation at the doses (0 , 5 , 10 or 15 ) Gray on the callus of four Triticum aestivum immature embryos genotypes (AL-Hashmiya , AL-Noor AL-Zahraa and AL-Mellad ) were studied . The fresh and dry weight for callus and shoot tips beside numbers and lengths of the shoots were used as indicators after 8 weeks . Results revealed that (AL-Noor and AL-Zahraa ) was superior by giving highest fresh and dry weight reached 274.2 and 269.2 mg and 26 and 24.3 mg respectively as compared with AL-Hashmiya and AL-Mellad. Moreover, the control treatment and the dose 10 Gray gave highest fresh weight reached 277.4 and 259.1 mg while the dry weight was highest in the control treatment and the dose 5 Gray. addition 10 Gra

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Publication Date
Sun Jun 01 2014
Journal Name
Baghdad Science Journal
Effect of Some Medicinal Plants on the Activity of Some Immunological Factors in Saliva and Serum of Type –?? Diabetics

The study involved 45 male and 45 females of diabetic patients type- ?? aged from 40-69years , and with the same numbers of males and females for control , all the patients and controls were without any periodontal diseases and without any systemic disease. Diabetic patients were divided in to three groups according to the degree of periodontitis , and the inflamed gingiva of all groups of diabetic patients were treated with the dried fruits powder (crude) of medicinal plants Quercus robur , Thuja occidenalis , Terminalia chebula, Anethum graveolens , respectively and mixture. Some immunological and antimicrobial factors (IgA, Lactoferrin , Lysozyme ) , were detected in serum and saliva of diabetic patients and the control

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Publication Date
Tue Mar 14 2023
Journal Name
Iraqi Journal Of Science
Cytological Studies of Irregular Meiotic Behavior on Some Medicinal Plants: in Al-Anbar, Iraq.

The present study on some wild medical taxa has been carried out from the cytological view-points by using iron-acetocarmine squashtechnique.The chromosome numbers of Diplotaxis harra n=13, Eruca sativa n=11, both Carduus pycnocephallus n=29 and Datura stramonium n=10 were the new reports. Avariety of abnormal Chromosomal behaviours including, early & late disjunction, laggards, bridges, stickiness, and disturbed polarity were observed, albite with low frequency. All sorts of chromosomal abnormalities were counted at I &II meiotic stages. The stickiness frequency was slightly higher than others abnormalities in most stages, The highest stickiness numbers were noted in telophase I/II of D. harra (8.88%), C. pycnocephalis (4.25%),

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Publication Date
Sun Dec 05 2021
Journal Name
Journal Of Education College Wasit University
Teaching Culture Strategies in EFL Classroom

الثقافة هي النظرية حول العالم والناسواساليب توزيع خبراته عليهم . انها الكل المعقد والتي تشمل المعرفةوالمعتقدات والفن والاخلاق والقوانين والعادات واية امكانيات وعادات يكتسبها الانسان كونه احد اعضاء المجتمع . ان ادراك ثقافة اللغة الاجنبية ينبغي ان يُنظرلها كمكونمهم في عملية تعليم / تعلم اللغة الانكليزية بوصفها لغةاجنبية وتتضمن الثقافة فهم داخلي وتدريجي للمساواة بين الثقافات ، ومنهن متزايد لثقافة الفرد

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