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ijs-7539
Extraction and Partial Purification of Lipopolysaccharide from Clinical Proteus mirabilis Isolate and Compared with Standard Bacteria
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Ninety five samples were collected from different samples (urine, ear and wounds swaps), from hospitals in Baghdad city. The results of cultural, microscopic , biochemical tests indicated that in urine samples E.coli have high occurrence frequency 19 (47.5%) followed by Proteus mirabilis 18(45%) and Klebsiella species 1 (2.5%), while in wounds samples each of Pseudomonas spp. and Proteus mirabilis 10 (25%) , then followed by E.coli with 5 (12.5%) and Klebsiella species 3 (7.5%). Ear swaps samples revealed that Pseudomonas aeruginosa 7 (46%) was the major bacterium followed by Proteus mirabilis 4(26.6).Sensitivity test against eleven antimicrobial agents was done for all of the Proteus mirabilis isolates (32 isolates). The results displayed that most of the isolates were resistant to Methicillin (96.8%), and Rifampin (93.7%) followed by trimethoprim–sulfamethoxazole (71.8%), chloramphenicol (62.5%), and cefazoline (59.3%). while the most effective antimicrobial agents against P. mirabilis were Imipenem (96.9%), Azetronam (81.3%), Azithromycin (71.9%) ,Ciprofloxacin (69%).Whereas a moderate effect appeared against both gentamycin and tobramycin in a percentage of (53.2 and 62.5) % respectively. More resistant isolate was selected, and lipopolysaccharide was extracted by hot EDTA method and the yield was (051) mg LPS from (22)g dry weight cell of pathogenic P.mirabilis and (95) mg as LPS from (16) g dry weight cell of standard bacteria were obtained. After partial purification ,chemical analysis of crud and partial purified LPS showed that the carbohydrate percentages were (35 , 44.3) % and (49 , 62)%, while the protein percentage (0.98 ,0.1) % and ( 1.3 ,0.1)% for the standard and isolated bacteria respectively ,whereas both extract appeared free from nucleic acid . Molecular weight of LPS was estimated and it was equivalent to (63095 and 70794) Dalton for the standard bacteria and pathogenic one respectively.

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Publication Date
Thu Jun 01 2017
Journal Name
Asian Biomedicine
The distribution of cutaneous leishmaniasis in Iraq: demographic and climate aspects
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AbstractBackgroundLeishmaniasis is endemic in Iraq, where both cutaneous and visceral forms of the disease are reported.ObjectivesTo determine the prevalence of cutaneous leishmaniasis (CL) and to identify associations of CL with age, sex, season, and provinces depending on some demographic and climatic aspects.MethodsThis study is retrospective and includes reported cases of infections using the available surveillance database taken from the Iraqi Ministry of Health for the years 2011, 2012, and 2013 for all provinces of Iraq.ResultsMen and boys were found to be at higher risk for CL compared with women and girls. The majority of cases were recorded among those in age groups 5–14 and 15–45 years old. Most cases were recorded from lowla

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Publication Date
Sun Jun 21 2020
Journal Name
Baghdad Science Journal
Detection of Aflatoxin B1 among Early and Middle Childhood Iraqi Patients
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      The study was conducted for the detection of Aflatoxin B1(AFB1) in the serum and urine of 42 early and middle childhood patients (26 male and  16 female ) with renal function disease, liver function disease, in additional to atrophy in the growth and other symptoms depending on the information within consent obtained from each patient, in addition to 8 children, apparently healthy, as  the control. The technique of HPLC was used for the detection of AFB1 from all samples. The results showed that out of 42 patient children, 19 (45.2%) gave positive detection of AFB1 in the serum among all age groups patients with a mean of 0.88 ng/ml and a range of (0.12-3.04) ng/ml. This was compared with the cont

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Publication Date
Wed Mar 30 2022
Journal Name
Iraqi Journal Of Science
Synthesis, Characterizations, and Magnetic Properties of Mixed Spinel Mg1-xZnxFe2O4 Ferrites
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    Mixed spinel Mg1-xZnxFe2O4 ferrites (where x = 0, 0.2, 0.4, and 0.6) nanoparticles were synthesized by using microwave-assisted combustion route. As-synthesized powdered samples were checked by XRD analysis, field emission-scanning electron microscopy, and vibration sample magnetometer to investigate the structural,   morphology, and magnetic properties, respectively. XRD results exhibited that the crystallite size increases with the decrease of Zn+2 ion concentration for series of mixed spinel Mg1-xZnxFe2O4 ferrite expect x=0.2. All the mixed spinel Mg1-xZnxFe2O4 ferrite has different gr

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Publication Date
Sun Dec 30 2018
Journal Name
Journal Of Engineering
Synthesis and Characterization of Titanium Dioxide Nanoparticles under Different pH Conditions
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Ethanol as a solvent, a precursor of titanium isopropoxide and a stabilizer of either hydrochloric acid or ammonium hydroxide was used to prepare a titanium dioxide aqueous solution. The aqueous solutions with different values of pH and the morphology of the resultant reaction of the nanoparticles of titanium dioxide were investigated. The X-ray diffraction showed that at low temperatures and with acidic solutions, rutile structures are more favorable to grow on titanium dioxide synthesized, while at low and average temperatures and with base solutions, anatase phase is more pronounced. The crystalline form and the re-confirmation of the crystallite size growth were observed by the scanning electron microscopy. The atomi

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Publication Date
Tue Jan 01 2019
Journal Name
Plant Archives
The role and importance of amino acids within plants: A review
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Publication Date
Tue Oct 01 2013
Journal Name
Journal Of Economics And Administrative Sciences
Institutional Structures and the Transformation of the Private Sector in Iraq
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            Requires economic work finding built institutional paint strategies and policies are formulated general economic and clarity in its stated objectives and the involvement of all economic institutions, political and stakeholders to discuss all the issues of economic, financial, monetary and analyzed for the purpose of renewal energies and determine the duties and responsibilities, leaving full freedom to the private sector in the formation of institutions to carry out his duties economic, and that the institutional structures to create the right climate for the implementation of its economic policies, which would facilitate the task of the private sector, and this h

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Publication Date
Sun Apr 08 2018
Journal Name
Al-khwarizmi Engineering Journal
Physical and Morphological Properties of Hard- Soft Ferrite Functionally Graded Materials
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  Functionally graded materials (FGMs), with ceramic –ceramic constituents are fabricated using powder technology techniques. In this work three different sets of FGMs samples were designed in to 3 layers, 5 layers and 7 layers. The ceramic constituents were represented by hard ferrite (Barium ferrite) and soft ferrite (lithium ferrite). All samples sintered at constant temperature at 1100oC for 2 hrs. and characterized by FESEM. Some physical properties were measured for fabricated FGMs include apparent density, bulk density, porosity, shrinkage and hardness. The results indicated that the density increase with the increase the number of layer. Lateral shrinkage is one of the important parameter f

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Publication Date
Tue Feb 01 2022
Journal Name
Iraqi Journal Of Science
A Study of compression strength and flexural strength for Polymer Concrete
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Polymer concrete were prepared by mixing epoxy resin with sand particles in three different grain size (150-300) , (300-600 ) and (600- 1200) μm respectively. The percentage of epoxy was 15%, 20 %, 25% and 30% wt of the total weight. Compression strength and flexural strength tests were carried out for the prepared samples.
The percentages of epoxy resin at 20% wt and 25% wt showed best mechanical properties for all grain sizes. These percentages were adopted to fill the voids between particles sand have two different size ranges (150-600) μm and {(150-300) & (600-1200)} μm respectively to obtain more dense material. The results showed that the strength of polymer composite at 20% resin is higher than 25% resin.

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Publication Date
Thu Mar 01 2012
Journal Name
Journal Of Economics And Administrative Sciences
The Absorptive Capacity of Operational Budget and Monetary Policy Fiscal Cost
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In light of the enquiry raised by the Economist Mary Finn in 1995 concluding that high utilization in absorptive capacity of the economy is of inflationary tendency for industrial countries due to the equality between high rates of utilization of absorptive capacity and resource – shortage conditions leading to price inflation, the same idea was used to prove that budget utilization of operational costs and elevating absorptive capacity at the expense of investment budget leads to inflationary tendency that becomes a burden on financing the step- in policy of the Central bank to control prices through its foreign currency reserves at a time when the economy turned  into an importer of non- tradable goods and being subject

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Publication Date
Sat Jul 28 2018
Journal Name
Journal Of Engineering
Experimental and Numerical Analysis of Expanded Pipe using Rigid Conical Shape
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The experimental and numerical analysis was performed on pipes suffering large plastic deformation through expanding them using rigid conical shaped mandrels, with three different cone angles (15◦, 25◦, 35◦) and diameters (15, 17, 20) mm. The experimental test for the strain results investigated the expanded areas. A numerical solution of the pipes expansion process was also investigated using the commercial finite element software ANSYS. The strains were measured for each case experimentally by stamping the mesh on the pipe after expanding, then compared with Ansys results. No cracks were generated during the process with the selected angles. It can be concluded that the strain decreased with greater angles of con

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