Ninety five samples were collected from different samples (urine, ear and wounds swaps), from hospitals in Baghdad city. The results of cultural, microscopic , biochemical tests indicated that in urine samples E.coli have high occurrence frequency 19 (47.5%) followed by Proteus mirabilis 18(45%) and Klebsiella species 1 (2.5%), while in wounds samples each of Pseudomonas spp. and Proteus mirabilis 10 (25%) , then followed by E.coli with 5 (12.5%) and Klebsiella species 3 (7.5%). Ear swaps samples revealed that Pseudomonas aeruginosa 7 (46%) was the major bacterium followed by Proteus mirabilis 4(26.6).Sensitivity test against eleven antimicrobial agents was done for all of the Proteus mirabilis isolates (32 isolates). The results displayed that most of the isolates were resistant to Methicillin (96.8%), and Rifampin (93.7%) followed by trimethoprim–sulfamethoxazole (71.8%), chloramphenicol (62.5%), and cefazoline (59.3%). while the most effective antimicrobial agents against P. mirabilis were Imipenem (96.9%), Azetronam (81.3%), Azithromycin (71.9%) ,Ciprofloxacin (69%).Whereas a moderate effect appeared against both gentamycin and tobramycin in a percentage of (53.2 and 62.5) % respectively. More resistant isolate was selected, and lipopolysaccharide was extracted by hot EDTA method and the yield was (051) mg LPS from (22)g dry weight cell of pathogenic P.mirabilis and (95) mg as LPS from (16) g dry weight cell of standard bacteria were obtained. After partial purification ,chemical analysis of crud and partial purified LPS showed that the carbohydrate percentages were (35 , 44.3) % and (49 , 62)%, while the protein percentage (0.98 ,0.1) % and ( 1.3 ,0.1)% for the standard and isolated bacteria respectively ,whereas both extract appeared free from nucleic acid . Molecular weight of LPS was estimated and it was equivalent to (63095 and 70794) Dalton for the standard bacteria and pathogenic one respectively.
This study aimed to determine the optimal conditions for extracting basil seed gum in addition to determine the chemical components of basil seeds. Additionally, the study aimed to investigate the effect of the mixing ratio of gum to ethanol when deposited on the basis of the gum yield which was1:1, 1:2, 1:3 (v/v) respectively. The best mixing ratio was one size of gum to two sizes of ethanol, which recorded the highest yield. Based on the earlier, the optimal conditions for extracting basil seed gum in different levels which included pH, temperature, mixing ratio seeds: water and the soaking duration were studied. The optimal conditions were: pH 8, temperature of 60°C, mixing ratio seeds: water 1:65 (w/v) and soaking duration of 30 min
... Show MoreIn this work, calcium ions were determined by the addition to the allyl chloride monomer resulting from bulk polymerization formation. To acquire the highest adsorption capacity, molar ratios of the template, monomer, and cross-linking agent, as well as solvents and multiple monomers were investigated. Scanning Electron Microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FTIR) were used to analyze the calcium ion polymer. The elution of calcium had a small effect on the surfaces of the three-dimensional network structure. Calcium (II) ions were successfully eluted using a mixture of methanol and acetic acid. The calcium adsorption capacities were 3.8145 and 9.01773 mol/g (Qmax), respectively. A Langmuir isot
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BACKGROUND: SARS-CoV-2 (COVID-19) is considered a highly infectious and life threatening disease. OBJECTIVE: The present paper aims to evaluate various aspects of preventive measures and clinical management of the scheduled visits for orthodontic patients to the dental clinics during the outbreak of COVID-19, and to assess how orthodontists dealt with this challenge. METHODS: Orthodontists in private and public clinics were invited to fill a questionnaire that addressed infection control protocols and concerns about clinical management of patients in the clinics during the pandemic. Frequncies and percentages of the responses were obtained and compared using Chi-square tests. RESULTS: About 77% of those working in private clinics, a
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The ligand [Potassium (E)-(4-(((2-((1-(3-aminophenyl) ethylidene) amino)-4-oxo-1,4dihydropteridin-6-yl) methyl) amino)benzoyl)-L-glutamate] was prepared from the condensation reaction of folic acid with (3-aminoacetophenone) through Schiff reaction to give a new Schiff base ligand [H2L]. The ligand [H2L] was characterized by elemental analysis CHN, atomic absorption (A.A), (FT-I.R.), (U.V.-Vis), TLC, E.S. mass (for spectroscopes), molar conductance, and melting point. The new Schiff base ligand [H2L], reacts with Mn(II), Co(II), Ni(II), Cu(II), Cr(III) and Cd(II) metal ions and (2-aminophenol), (metal : derivative ligand : 2-aminophenol) to give a series of new mixed complexes in the general formula:- K3[M2(HL)(HA)2], (
... Show MoreThe ligand [Potassium (E)-(4-(((2-((1-(3-aminophenyl) ethylidene) amino)-4-oxo-1, 4-dihydropteridin-6-yl) methyl) amino) benzoyl)-L-glutamate] was prepared from the condensation reaction of folic acid with (3-aminoacetophenone) through Schiff reaction to give a new Schiff base ligand [H2L]. The ligand [H2L] was characterized by elemental analysis CHN, atomic absorption (AA),(FT-IR),(UV-Vis), TLC, ES mass (for spectroscopes), molar conductance, and melting point. The new Schiff base ligand [H2L], reacts with Mn (II), Co (II), Ni (II), Cu (II), Cr (III) and Cd (II) metal ions and (2-aminophenol),(metal: derivative ligand: 2-aminophenol) to give a series of new mixed complexes in the general formula:-K3 [M2 (HL)(HA) 2],(where M= Mn (II) and Cd
... Show MoreThe current research seeks to achieve several objectives, including knowing the extent of the audit directorate of the Ministry of Construction, Housing and General Municipalities of the International Standard (ISO19011:2018) regarding determining the efficiency and evaluation of auditors and diagnosing the gap between requirements and application and knowing the reasons for not applying some of the items in the standard, starting from the problem, The field raised the following question (Does the audit directorate determine the efficiency and evaluation of auditors according to the standard ISO19011:2018?), and the importance of research lies in determining the return that can be achieved by the directorate through its application of stand
... Show MoreThis study was designed to stand on the most important causes of contamination in sesarean section in women by the aerobic bacteria species the bacteria isolates were diagnosed in the Maternity and Children Hospital in the Qadsiya province and in the special clinics. Sixty five persent of women having cesarean operation were contaminated with different bacteria in different ratios. Staphylococcus aureus showed the higher percentage (62.4) , Pseudomonas.aerogenosa 18% , E.coli 9.5% and Proteus.spp (6,4%).While Streptococcus pyogens showed lowest percentage 3.6% . The results of antibiotic sensitivity test for all bacteria isolates showed Chloramphenicol and Ami
... Show MoreFructophilic lactic acid bacteria (FLAB) isolated from honeybee’s nectar stomach represent an important source of antimicrobial substances that has been focused on nowadays. In this study, these FLABs were tested for their antibacterial activity in vivo as well as the detection of Interleukine -17 in the serum of rabbits ingested with FLAB isolates. Fructobacillus fructosus FH1, Fructobacillus fructosus FH8 and Lactobacillus kunkeei SHGH-7 FLAB isolates were used against wound infections caused by Pseudomonas aeruginosa and Staphylococcus aureus (MRSA) in rabbit skin . Enzyme linked sorbent immunoassay (ELISA) was used to detect the levels of Interleukine-17 in the sera of rabb
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A total of 50 wells water samples were collected from 10 wells in Abu Ghraib site/ Baghdad for detection of coliform, fecal coliformand and pseudomonas aeruginosa bacteria, and the pollution of toxic ions (No3-, B+3, pb+2 and Cd+2 ) in wells water. Results showed microbial pollution by coliform, fecal Coliform bacteria in wells water when using presumptive, confirmed and complete tests, P. aeruginosa bacterium was isolated and identificated on Cetrimide Agar media, estimated the Most Probable Number (MPN) of coliforms and P. aeruginosa, results showed difference in mean of (MPN) of wells water. Most of toxic ions concentrations were low comparing with the recommended hygienic standards from the World Health Organization (WHO). Wells wate
... Show MoreThe aim of the present study was assess the antimicrobial effect of
Peganumharmala L seeds extracts by ethanol (80%) on gram negative and gram
positive bacteria and four concentrations (25, 50, 75 and 100) mg/ml were prepared.
Four clinical isolates of bacteria were used; two were positive and two were
negative bacteria; that include: Bacillus, Staphylococcus aureus, Pseudomonas
aeruginosa and Escherichia coli. The results showed that all concentration that have
been used had antimicrobial effect against gram negative and gram positive bacteria
and the best concentration that have the best antimicrobial effect was 100 mg/ml and
the effect of alcoholic extraction was greater on gram positive bacteria than gram
n