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Using of Species-Specific Primers for Molecular Diagnosis of in Vitro Promastigotes of Leishmania donovani

Visceral Leishmaniasis (VL) is a disseminated protozoan infection caused by Leishmania donovani parasites which affects almost half a million persons annually. Classical diagnosis methods of VL still not very sensitive and time consuming. In this study, we reported the success of polymerase chain reaction (PCR) method to identify L. donovani based on kinetoplast deoxyribonucleic acid (kDNA) for the diagnosis of the parasite using in vitro promastigote cultures. LdI species - specific primer was used to identify L. donovani and the result showed a single band of about ~600bp. It can be recommended that this primer is to be used for detection the visceral L. donovani.

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Publication Date
Sat Nov 01 2008
Journal Name
Journal Of Physical Science
Spectrophotometric Determination of Allopurinol in Tablet Formulation

A new spectrophotometric method for the determination of allopurinol drug was investigated. The proposed method was based on the reaction of the intended drug with catechol and Fe(II) to form a blue soluble complex which was measured at λmax 580 nm. A graph of absorbance versus concentration shown that Beer’s law was obeyed over the concentration range of 2–10 μg ml–1 with molar absorptivity of 9.4 x 103 l mol–1 cm–1 and Sandell sensitivity of 1.4 x 10–2 μg cm–2. A recovery percentage of 100% with RSD of 1.0%–1.3% was obtained. The proposed method was applied successfully for the determination of allopurinol drug in tablets with a good accuracy and

Publication Date
Wed May 15 2013
Journal Name
Asian Journal Of English Studies
Strategies of Politeness in English Medical Conversations

BN Rashid, Ajes: Asian Journal of English Studies, 2013

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Publication Date
Thu Feb 07 2019
Journal Name
Journal Of The College Of Education For Women
Social discipline in the building of society

In order to get an idea about things closely, we should look at the role of social discipline
in the building of society from civilization cultural viewpoint. The real weigh of the societal
opinion is not measured by the number of its population or its area but the real value lies in its
civilization cultural accumulative experience inherited by the generations, which determine its
distinguished character. That is based upon the social construction in which the methods of
socialization and political, economic, cultural and religious stability and enhance the national
and ethical character of self-realization.
In this research, we try to give an analysis for the obstacles in political economic and
religious social

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Publication Date
Sat Dec 30 2023
Journal Name
Gsc Biological And Pharmaceutical Sciences
Checklist of insects- borne diseases in Iraq

This study presents an updated checklist of the dipteran-borne diseases in Iraq, together with their original name combinations and synonyms. According to this checklist, 152 species, 40 genera within 14 families. Furthermore, minor corrections were applied to some authors’ names and years of publication.

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Publication Date
Fri Sep 11 2020
Journal Name
Iop Conference Series: Materials Science And Engineering
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Publication Date
Mon Mar 29 2021
Journal Name
Journal Of Engineering
Behavior of Defective Cast in Place Piles

This paper deals with testing defected model piles in the soil in order to study their behavior. In this respect, the results of model pile tests are discussed either geotechnically or structurally according to the type of failure.   Two parameters were studied in order to evaluate the general behavior of defective piles. These parameters include the defect location and the defect type for floating and end bearing pile. The results of the experimental work indicated that the critical case for floating pile is seen to be when the defect of (5%) at the first third of the pile length at which the decrease in the bearing capacity is about (21%), while the decrease in the bearing capacity is found to be (14%) and (10%), when

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Publication Date
Sun Mar 07 2010
Journal Name
Baghdad Science Journal
Biochemical Study of Gallstones Compositions in Iraqipatients

The aim of this study is to determine the organic and inorganic components of bile and gallstones in Iraqi patients. Forty seven patients were included in this study with mean age (53+7) years and BMI (30.82+4.18) Kg/m2. Bile was classified according to its corresponding stones into: Bile of Mixed stones and Bile of pigment stones. IR spectra were studied for both types of stones and their bile in addition to biochemical analysis for organic and inorganic components. The organic components include: (cholesterol, bilirubin, bile salts, and phospholipids), while inorganic components include salts of: (calcium, phosphorus, iron, cupper and magnesium). The results reveal to there was significant low levels (p<0.005) of bile salts and phospholi

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Publication Date
Thu May 25 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Some Types of Compactness in Bitopological Spaces

  In this paper, we give the concept of N-open set in bitopological spaces, where N is the first letter of the name of one of the authors, then we used this concept to define a new kind of compactness, namely N-compactness and we define the N-continuous function in bitopological spaces.         We study some properties of N-compact spaces, and the relationships between this kind and two other known kinds which are S-compactness and pair-wise compactness.

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Publication Date
Wed Mar 29 2017
Journal Name
Iraqi Journal Of Pharmaceutical Sciences ( P-issn 1683 - 3597 E-issn 2521 - 3512)
Misuse of appetite- stimulant drugs in Babylon#

Drug misuse is defined as using of drugs for a non-therapeutic or non-medical purpose. In Iraq drug misuse is a major problem because almost any drug can be easily obtained from pharmacies. Appetite- stimulant drugs are example of drugs that are widely used without a prescription. The study included 230 patients who use these drugs in Babylon. A questionnaire included the following questions ; age, sex, marital state, the reason for use the drug , whether the drug is prescribed by physician or not , type of drug used , the frequency of daily dose and lastly the extent of side effects of the drugs used. The results showed that the age range of 35% of subjects were (17-21) years old and 70% of participants were females. The study also show

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Publication Date
Thu Aug 03 2023
Journal Name
Journal Of Legal Sciences
Possession of Unlicensed Weapons in Comparative Laws

The crime of possessing unlicensed weapons is considered a very dangerous crime for individuals and the entire state system، and possession of unlicensed weapons is the backbone of the existence of organized crime in society، given the serious consequences of this crime when used، which are loss of life and bodily injury to individuals، as well as On the psychological effects of fear، insecurity and loss of confidence in the security services. The study dealt with possession in criminal jurisprudence as well as in civil jurisprudence، and the definition of weapons and their types was also addressed، and we noticed through the research that sound and light weapons، unusable weapons and weapon parts are not considered a firearm and

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