Multi-drug resistance in Listeria monocytogenes is considered a major public health problem associated with foodborne outbreaks and causes high hospitalization and mortality rates. This study aimed to investigate the antimicrobial resistant genes among Listeria monocytogenes isolated from meat and clinical samples. Phenotypically, the isolates were tested for their susceptibility against the 12 most commonly used antimicrobials in veterinary and human therapy via the disc diffusion method, while conventional PCR was performed to study the presence or absence of 14 resistance genes predicted in L. monocytogenes isolates. The study established that 30(66.66%) of L. monocytogenes isolates showed phenotypic multi-drug resistance against at least three antimicrobial classes. Furthermore, high resistance frequencies were reported among commonly used antibiotics for listeriosis therapy. The present study revealed that the investigated isolates show resistance against tetracycline 33(73.3%), ampicillin 29(64.4%), penicillin 28(62.2%), erythromycin 26(57.8%), and gentamycin, clindamycin and vancomycin 24(53.3% each). Of the 45 L. monocytogenes isolates studied, 37(82.2%) were phenotypically susceptible to meropenem, followed by ciprofloxacin 36(80.0%) and SXT 30(66.7%). PCR amplification of antimicrobial resistance genes established the occurrence of antibiotic resistance genes in all studied L. monocytogenes isolates. Notably, 41(91.11%) of these isolates exhibited more than five resistance genes. Surprisingly, penA and ampC were detected in all L. monocytogenes strains 45(100%), followed by ermB 44(97.8%), tetA 38(84.4%), tetG 32(71.1%), and vanB 30(66.7%). Moreover, vanA 22(48.9%) and tetB 18(40.0%) were detected less frequently. The lowest incidences of resistance genes were observed in L. monocytogenes carrying tetD 4(8.9%) and cmlA 6(13.3%). In conclusion, the study demonstrates that the majority of L. monocytogenes from human and meat samples displayed a high index of resistance to a variety of agents used for clinical listeriosis treatment adding further burden to the existing global antibiotic resistance problem.
This paper presents a study (experimentally) for strengthening reinforced concrete (RC) beams with Near-Surface-Mounted (NSM) technique. The use of this technique with CFRP strips or rebars is an efficient technology for increasing the strength for flexure and shear or for repairing damaged reinforced concrete (RC) members. The objective of this research is to study, experimentally, RC beams either repaired or strengthened with NSM CFRP strips and follow their flexural behavior and failure modes. NSM-CFRP strips were used to strengthen three RC beam specimens, one of them was initially strengthened and tested up to failure. Four beam specimens have been initially subjected to preloading to 50% and 80% of ultimate load. Two of the sp
... Show MoreThe current research aims to measure the job satisfaction of educational counselors in the general directorate of education of the second Rusafa in the ministry of education of Iraq. Moreover, it aims to identify the significant differences in job satisfaction according to the gender (Male-Female), the length of service (less than 15 years more than 15 years), and the relationship between these two variables. To achieve the objectives of the research, the researcher developed a scale to measure job satisfaction. This tool was applied to sample of (100) educational counselors selected randomly. The results showed that educational counselors have job satisfaction, in which males are more satisfied in their job than females. The results als
... Show MoreAbstract:Two-dimensional crystal has been achieved and controlled with the aid of DC electric field applied between two electrodes at 5 millimeters separating distance between them. Sol-gel method has been used to prepared nanosilica particle which used in this work as well as TiO2 nanopaowder. The assembly of the silica particles is due to the interaction between the electrical force, the particles dipole, and the interaction between the particles themselves. When a DC voltage is applied, the particles accumulated and crystallized on the surface between the electrodes. The Light diffraction demonstrates that the hexagonal crystal is always oriented with one axis along the direction of the field. The particles disassemble when the field is
... Show MoreObjective: Assess type 2 diabetic patients’ knowledge regarding preventive measures of diabetic foot. Find out the relationship between of type 2 diabetic patients’ knowledge regarding preventive measures of diabetic foot with certain sociodemographic characteristics
Methodology: A descriptive study was carried out from (2nd January 2022 to 26th March 2022). A non –probability (purposive) sample of (60) adult patients who are diagnosed with type2 diabetes mellitus these patients have met the study criteria which was selected from Imam AL-Hussein Medical-City. The study instrument consist of two section: (Demographic Information Sheet, and Foot Care Outcome Expectation
... Show MoreSolar photovoltaic (PV) system has emerged as one of the most promising technology to generate clean energy. In this work, the performance of monocrystalline silicon photovoltaic module is studied through observing the effect of necessary parameters: solar irradiation and ambient temperature. The single diode model with series resistors is selected to find the characterization of current-voltage (I-V) and power-voltage (P-V) curves by determining the values of five parameters ( ). This model shows a high accuracy in modeling the solar PV module under various weather conditions. The modeling is simulated via using MATLAB/Simulink software. The performance of the selected solar PV module is tested experimentally for differ
... Show MoreThis paper describes a modified mathematical method that used for controlling and generating three dimensional surfaces based on different axes (X, Y, and Z) and free axis. The main aim of the proposed method is to allow the designer to change the shape of the surface to the desired one without changing the original data points which is presented in the earlier version of this paper under title "3D Surface Reconstruction of Mathematical Modelling Used for Controlling the Generation of Different Bi-cubic B-Spline in Matrix Form without Changing the Control Points". The proposed method has been done by changing the t and s, parameters value that are assigned secretly by the designer. Therefore, in case off the control points have been disc
... Show MoreThe present study aims to remove nickel ions from solution of the simulated wastewater using (Laminaria saccharina) algae as a biosorbent material. Effects of experimental parameters such as temperature at (20 - 40) C⁰, pH at (3 - 7) at time (10 - 120) min on the removal efficiency were studied.
Box-Wilson method was adopted to obtain a relationship between the above three experimental parameters and removal percentage of the nickel ions. The experimental data were fitted to second order polynomial model, and the optimum conditions for the removal process of nickel ions were obtained.
The highest removal percentage of nickel ions obtained was 98.8 %, at best operating conditions (Temperature 35 C⁰, pH 5 and Time 10 min).
The current study designed to determine the effect of Glucosamine sulfate on the liver tissue of Albino mice .the study included (40)mice divided in to 4 groups(control group had distilled water orally ).The other groups treated with(1000,2000,3000)ml/k .respectively for 8 week .the liver have been taken from dissected animal for microscopic preparation to study the histological changes .Frequently histopathologicale changes appeared in the liver tissue of the exposure groups during (4-8)week .This changes depends on (Dose and Time ). The effects were Congestion ,Infiltration ,Swelling ,Vaculation ,Hyalinization , Amyloid and Necrosis.