In this study, we investigated the ability of nanoliposomes preparation, as a nanoadjuvant, to entrap soluble Leismania donovani antigens (SLAs) and release in vitro. The parasite reactivation was carried out when inoculated into Rosewell park memorial institute media (RPMI) and incubated at 23 °C for 4 days. L. donovani promastigote inoculum (104 cell / ml) of 4 days was used to inoculate modified medium of Saline - Neopeptone and Blood agar 9 (SNB 9) to produce promastigote mass. SLAs were extracted from the promastigotes ghost membrane after fourth passages of subculturing in SNB. The membrane pellet obtained was suspended in 5 mM Tris buffer (pH 7.6) and sonicated three times at 4 °C and entrapped in freshly prepared nanoliposomes. Lipids mixture of 4mM Phosphatidylcholine, 2.2 mM Cholesterol and 0.55 mM Phosphatidylethanolamine in a ratio of 7:2:1 were used to prepare nanoliposome. Physio-chemical characterizations of prepared nanoliposomes was performed by using Scanning Electron Microscope (SEM) , Atomic Force Microscope (AFM) and Zeta Potential assays to determine the size, morphology and charge. The efficiency of freshly prepared nanoliposoms to entrap SLAs was determined by measuring the nanoliposome efficiency entrapment (EE). The percentage of EE was 50 and 27.5 of SLAs entrapped nanoliposomes prepared by Sephadex G25 and Sephadex G75, respectively. Moreover, stability of SLAs entrapped nanoliposomes was examined at 4 and 37 °C as a storage temperature.
In this work, the possibility of utilizing osmosis phenomenon to produce energy as a type of the renewable energy using Thin Film Composite Ultra Low Pressure membrane TFC-ULP was studied. Where by forward osmosis water passes through the membrane toward the concentrated brine solution, this will lead to raise the head of the high brine solution. This developed static head may be used to produce energy. The aim of the present work is to study the static head developed and the flux on the high brine water solution side when using forward and reverse osmosis membranes for an initial concentration range from 35-300 g/l for each type of membrane used at room temperature and pressure conditions, and finally calculating the maximum possible po
... Show MoreBioethanol is an attractive fuel with higher potential for energy security and environmental safety. Olive solid residues were used as a raw material for the production of bioethanol through the use of different preliminary treatments . Separate treatments with cellulose, hydrochloric acid (HCl 5%), sulfuric acid (H2SO4 2%), and liquid ammonia NH4OH (20%) were used to convert cellulose and hemicellulose into monosaccharaides. The production of ethanol was observed during the fermentation process using R. minuta under anaerobic conditions. After 3 days of fermentation, lowest concentrations of ethanol of 0.233, 0.249, 0.261, and 0.275 g/ l were produced from ol
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In the present work, the pollutants of the municipal wastewater are reduced using Chlorella vulgaris microalgae. The pollutants that were treated are: Total organic carbon (TOC), Chemical oxygen demand (COD), Nitrate (NO3), and Phosphate (PO4). Firstly, the treatment was achieved at atmospheric conditions (Temperature = 25oC), pH 7 with time (1 – 48 h). To study the effect of other microorganisms on the reduction of pollutants, sterilized wastewater and unsterilized wastewater were used for two types of packing (cylindrical plastic and cubic polystyrene) as well as algae's broth (without packing), where the microalgae are grown on the packing then transported to the wastewater for treatment. Th
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Hydro cracking of heavy oil is used in refinery to produce invaluable products. In this research, a model of hydro cracking reactor has been used to study the behavior of heavy oil in hydro cracking under the conditions recommended by literature in terms lumping of feed and products. The lumping scheme is based on five lumps include: heavy oil, vacuum oil, distillates, naphtha and gases. The first order kinetics was assumed for the conversion in the model and the system is modeled as an isothermal tubular reactor. MATLAB 6.1 was used to solve the model for a five lump scheme for different values of feed velocity, and temperature.
The main objectives of this research is to extract essential oil from: orange ( citrus sinensis), lemon( citrus limon) and mandarin( citrus reticulata) peels by two methods: steam distillation (SD) and microwave assisted steam distillation (MASD), study the effect of extraction conditions (weight of the sample, extraction time, and microwave power, citrus peel type) on oil yield and compare the results of the two methods, the resulting essential oil was analyzed by Gas Chromatography (GC).
Essential oils are highly concentrated substances used for their flavor and therapeutic or odoriferous properties, in a wide selection of products such as foods, medicines and cosmetics. Extracti
... Show MoreIn this work, the extraction of glycyrrhizin from Licorice using bulk liquid membrane technique was developed and optimized. The effect of various parameters such as pH of stripping and donor solutions, temperature, stirring speed and kinetic parameters were investigated. Moreover, to study the impact of the polarity of membrane solvent, two types of extraction solvents were used as a membrane solvent: n-Hexane was used as a non-polar solvent and 1-Hexanol was as a polar solvent. The optimum extraction condition was found (95.53%) using 1-Hexanol, rotating speed was 400 rpm, and pH of the acceptor and donor solutions were 8 and 5.5, respectively. The reaction kinetics constants ( and ) for the transport of glycyrrhizin from the donor pha
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Saccharomyces Cerevisiae cells were immobilized in calcium alginate beads and activated charcoal for use in the
production of ethanol from batch fermentation of sugar beet waste. Treatment of the waste with NaOH to increase the
ability of lignocellulose material to hydrolysis by acid (2N H2SO4) to monosaccharide and disaccharide (mainly glucos).
The high reducing sugar concentration obtained was equal to 9.2gm/100ml (10Brix) after treatment. Fermentation
parameters, are (pH, glucose concentration (2.5-25 gm/100ml), immobilized agent concentration (2.5-25 gm/100ml)
were studied to find the optimum physiological condition. And the highest ethanol concentration obtained from the
fermentation in the presence of 20%(wt/v) ca