This study aimed for isolation and identification of Candida glabrata from specimens of blood collected from national center of hematology /Al - mustansiriya University of immunocompromised patients infection of candidemia after diagnosis by doctor. Results showed the morphological features on many media SDA and Corn meal and differential CHROMagar. Colonies appear small and pink pale to dark. Microscopic exam of show that C. glabrata not form pseudohyphae and not produce germ tubes, the cell size 2 - 3 microns, on the other hand, Biochemical test of C. glabrata have high ability for fermentation of glucose within three hours and trehalose given false positive within two hrs., and the Urease test given negative result, on the other hand , The results indicated that the DNA of isolated strains was extracted successfully. The results of PCR amplification which was performed on that DNA extracted from all isolates were confirmed by the electrophoresis analysis. The primer amplified of the ribosomal DNA in all strains tested, resulting in amplified products of approximately 610 bp in C. glabrata.
An abortion that occurs spontaneously is known as a miscarriage. Various effectors associated with abortion such as Genetic and uterine anomalies, Endocrinopathy, immunological dysfunctions, infectious agents, environmental contaminants, psychogenetic elements, and endometriosis. Maternal infections considered the main reason for pregnancy wastage in females with Bad Obstetric History (BOH). Candida albicans is a dimorphic fungus that can grow as yeast or filamentous cells and considered one of the limited species of the Candida genus that cause humans candidiasis. It is an opportunistic fungus that responsible for mucosal infections in the mouth and genital tract. Excessive growth of C. albicans will follow with Vulvovaginal candid
... Show MoreThis thesis aims to show the effects of the development of the traditional manual system of the tax accounting process to the electronic system by the activation of the tax identification numbers (TINs) mechanism. The impact of this development is facilitating the tax accounting process, tax fraudand thus increasing the tax outcome.To prove the research hypothesis, an electronic system was designed based on income tax report, estimation note of individuals, in additional to using Adobe Dreamweaver application to write PHP, HTML, Javascript, and CSS web languages to implement the proposed system. The research reached a set of conclusions, the most important of which is; not enough the communication methods between the Genera
... Show MoreBackground: The aim of the study was to determine the prevalence of CMV infection among HIV / AIDS patients in relation to disease progression, and to study the mortality during the period of the study.
Patients and Methods: The study included 155 HIV/AIDS patients (148 HIV- infected and seven AIDS patients) and 122 apparently "healthy" controls. CMV (IgG and 1gM) antibodies were determined by ELISA. The patients were followed up for a period of nine months, and retested frequently for development of active CMV infection.
Results: The prevalence of CMV (IgG) antibodies in the HIV/AIDS patients was 100%. A significant higher prevalence of CMV (IgM) among AIDS patients (42.9°/6) than among HIV infected
One hundred and sixty samples from saliva and dental plaque were sellected from patients with caries active at ages from (4-65) years from Umm Qasr Primary School and Al-Ameria Specialist Dental Center in Baghdad. 15 isolates belong to Streptococcus mutans were identified by biochemical tests and Vitek 2 compact system while 22 isolates identified by using Polymerase Chain Reaction ﴾PCR﴿ techniques and sequencing of 16SrRNA with 120 bp by using 16SrRNA the result confermed that these isolates were belong to S.mutans.
During the period from September 2013 till the end of July 2014 ,a total of 340 birds Passer domesticus were collected from Tikrit city . The study revealed the infection of birds with seven species of cestoda helminthes , belonging to the genus Raillietin . These species included R. tetragona , R. echinobothrida , R. cesticellus and R. ransomi with prevalence infection of 36.1% , 30.1% . 15.0 % and 1.8 % respectively . And the genus Choanotaenia . These species included C. infundibulum and C. passerine with pervatence infection of 15.0% and 0.6% respectively . And the genus Anonchotuenia . The species included A.globate with prevantence infection 1.2% .
... Show MoreA total number of 68 water samples was revealed 20 isolates being Staphylococcus aureus. Irrigation water isolates represented 25% of isolates while wastewater 75%. all isolates were identified by morphological, microscopial, biochemical tests and VITEK®2 Compact. Bacterial isolates were subjected to 16 antibiotics, all irrigation water and wastewater isolates were resistant to penicillin while they were fully sensitive to Ciprofloxcin. Irrigation water isolates showed relatively greater multi-drug resistance than wastewater, wherein irrigation water isolates showed 100% multi-drug resistance while wastewater isolates showed 73.3% multi-drug resistance, indicating the ability of S. aureus MDR to move from one site to another, which means t
... Show MoreObjective: Synthesis, Characterization of formazan derivatives and studies the antioxidant activity of prepared compounds and molecular docking. Methods: In this study, formazan compounds (III–XIV) were produced by combining Schiff base compounds (I), (II) with diazonium salts resulting from reactions of different aromatic amines with sodium nitrate in the presence of Con.HCl at 0–5°C. When isonicotinic acid hydrazide reacts with (N,N-dimethylbenzaldehyde, 4-hydroxy-3-methoxybenzaldehyde) in the presence glacial acetic acid as a solvent Schiff base compounds are created. Results: The prepared compounds were identified by FT-IR, 1H NMR, 13C NMR, then the antioxidant activity of the derivatives and molecular docking were studied. D
... Show MoreA total of 96 stool samples were collected from children with bloody diarrhea from two hospitals in Baghdad. All samples were surveyed and examined for the presence of the Escherichia coli O157:H7 and differentiate it from other Non -Sorbitol Fermenting Escherichia coli (NSF E. coli). The Bacterial isolates were identifed by using morphological diagnostic methods, Samples were cultured on liquid enrichment medium, incubated at 37C? for 24 hrs, and then cultured on Cefixime Tellurite -Sorbitol MacConkey Agar (CT- SMAC). 32 non-sorbitol fermenting bacterial isolates were obtained of which 11 were identified as Escherichia coli by using traditional biochemical tests and API20E diagnostic system without differentiation between
... Show MoreGroundwater is considered as one of the most important sources of fresh-water, on which many regions around the world depend, especially in semi-arid and arid regions. Protecting and maintaining groundwater is a difficult process, but it is very important to maintain an important source of water. The current study aims to assess the susceptibility of groundwater to pollution using the DRASTIC model along with the GIS environments and its tool boxes. A vulnerability map was created by relying on data collected from 55 wells surveyed by the researchers as well as archived records from governmental institutions and some international organizations. The results indicate that the region falls into three vulnerability functional zones , namely
... Show MoreBrucellosis is possess a significant public health problem in Baghdad. In this study, we investigated the potential role of the PCR assay in detection of Brucella species, from patients suspect to have brucellosis, using blood samples in both human and animal. To establish a PCR technique for diagnosis of active brucellosis in our samples, DNA extraction was carried out using a commercial kit, and a laboratory extraction procedure. PCR amplification was done using 1 set of primers: B4/B5 for Brucella species. Extraction of Brucella DNA using the commercial kit was successful. The laboratory extraction was successful and more economic. A total of 178 peripheral blood
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