Cutaneous Leishmaniasis (CL) is an endemic disease and one of the major health problems in Iraq. Leishmania tropica is known as the causative agent of Cutaneous Leishmaniasis in Baghdad.The classical serological methods of diagnosing leishmaniasis is a poor sensitivity especially for the sub genus and time consuming Here we have investigated two primer pairs, one specific for Leishmania as genus and the primer specific for the species of L. tropica to be detected by polymerase chain reaction (PCR).Samples were collected from (AL-karama Teaching Hospital) and whole genomic DNA was extracted from axenic promastigotes.The extracted DNA was amplified by PCRwith two KDNA primer pairs, for genus specific (13A/13B) and (Lmj4/Uni21) to identify the specific species of cutaneous leishmaniasis. Amplified PCR products then run on gel electrophoresis and two visible bands of the two primers were seen, 120 and 800 bp, respectively. Our results indicate that the PCR technique is sensitive and specific for the detection and differentiation of cutaneous leishmaniasis agents and can be recommended to applied in hospitals and research centers.
This study concluded detection of Toxoplasma gondii in milk, immunologically by using Elisa and nested PCR)nPCR (based on B1 gene, also to investigate the effect of toxoplasmosis, parity, breed and flock on some milk composition in the Iraqi local and Shami goats in the middle of Iraq. A total of 80 milk samples of the lactating goats were collected. Results of this study showed the prevalence of Toxoplasmosis was 21.25% and 28.75% by Elisa and nPCR respectively without significant differences. The sensitivity of Elisa was a low (30.43%) whereas the specificity was a high (82.45%). The degree of agreement estimated by Kappa coefficient revealed a slight agreement (0.14) between two methods. The results indicated that goats infected
... Show MoreIn this study negative result of real-time reverse transcription-QPCR (RT-PCR) assay
tests of Influenza virus of nasal screetion and throat swap samples of Iraqi patients
hospitalized with signs and symptoms of an upper respiratory tract infection in Central
Republic Health Laboratory in Iraq were tested for Respiratory Syncytial Virus
infection by RT PCR .Positive samples was 4 out 0f 20 were used .Viral isolation was
done on a monolayer of 70-80% confluent Human Lung Carcinoma Cells (A549) cell
line and incubated at 33ºC for 4 days .Syncytia was observed in 3 positive samples.
The identification and sequencing of Amyloid Precursor Protien (APP) and presenilin (PS) opened the door for the engineering of transgenic mouse models to study pathogenic mechanisms of Alzheimer Disease (AD). The first successful mouse models over-expressed human APP with an Familiar AD (FAD) linked mutation in the brain. These mice exhibit Aß plaques, neuron loss, dystrophic neurites, inflammatory responses, learning impairments and deficits in synaptic transmission and/or long-term potentiation. The genotypes of all offspring of APP/PS1 mutant mice are analysed by Polymerise Chain Reactions. Generally there are two possibilities to analyse the DNA. The First, primers for APP or PS1 was used separately assuming that both genes are int
... Show MoreRespiratory tract infections in sheep are among the important health problems that affect all sheep ages around the world. Nine bacterial isolates obtained from sheep with respiratory tract infections were selected to be used in the current study. The isolates included 3 Staphylococcus aureus, 4 Klebsiella pneumoniae, and 2 Pseudomonas aeruginosa. Following the primers design by the Primer3Plus software tool and optimization of the conventional polymerase chain reaction (PCR), the primers were validated for their use in the multiplex PCR experiments. The MFEprimer program was used to check the suitability of the primer set combinations for multiplex PCR. The MFEprimer software was successful in designing the multiplex-PCR experiments and de
... Show MoreThe dipstick test was evaluated for sero-diagnosis of visceral leishmaniasis. We compared two types of dipstick (rKE16, rK39) tests . The sensitivity of both tests were determined using sera from fifty-two children suspected of having visceral leishmaniasis and thirty healthy children as a control group collected from Central Teaching Hospital of Pediatric in Baghdad. Fifty (96.15%) cases were confirmed to have infection by rKE16 dipstick test while, fourty-six (88.46%) cases were positive by rK39 dipstick test. Non of the sample taken from healthy control showed reactivity in any of these tests. The study indicated that rKE16 test had better sensitivity than rK39 in the diagnosis of VL(100%) ,(92 %) respectively.
J Fac Med Baghdad 2023; Vol.65, No. 3 Received:March., 2023 Accepted: June. 2023 Published: Oct. 2023
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Vitamin D3 deficiency is regarded as a public health issue in Iraq, particularly during the winter. Sun exposure is the main source of vitamin D3, where the surface ultraviolet (UV) radiation plays an important role in human health. The amount of time that must be spent in the sun each day was determined for the amount of exposed skin, for all skin types, with and without sunscreen under clear sky conditions in the city of Baghdad (Long 44.375, Lat 33.375). UV index data was obtained by TEMIS satellite during the year 2021. From data analysis, we found that most days during the year were within the high level of ultraviolet radiation values in the city of Baghdad, and most of them were during the summer, where the person n
... Show MoreEvaluation of Dot. ELISA test for Diagnosis Visceral Leishmaniasis in Infected Children
For the first time in Iraq, this study was conducted to evaluate the usefulness of Dot.ELISA, for detecting anti - Leishmania donovani antibodies in serum samples from suspected patient (children under 8 years ) with Visceral Leishmaniasis V.L.. Sera from 73 V.L. , 60 Healthy controls, and 57 patient with other parasitic diseases other than V.L. (Amoebiasis, Giardiasis , Toxoplasmosis, Schistosomiasis , Hydatidosis, Ascariasis , Lupus Erythromatosus , Viral Hepatitis, and Cutaneous Leishmaniasis) were examined. Anti Leishmania donovani antibodies detected in 71 out of 73 suspected Visceral Leishmaniasis . Data of this study showed that infection in male group was more than female group. Result o
... Show MoreThe present study was conducted to estimate the incidence, clinical findings, cytological and histopathological characteristics of spontaneously occurring skin neoplasms in dogs. A total of 40 grossly suspected cases of cutaneous and subcutaneous tumors were gathered during the period from July 2016 to August 2018 from male and female dogs in Baghdad city. Dogs with skin neoplasia revealed various clinical signs, and their ages were older than 5 years to 15 years. German shepherd 30% followed by Terrier dogs 25% were more influenced than other breeds. Concerning tumor features, the majority of neoplasms had solitary lesion 70%, regular shapes 65% with black color 55%. The tumors frequently occurred on fore-limbs and abdomen, and 80% of them
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