For the first time in Iraq, this study was conducted to evaluate the usefulness of Dot.ELISA, for detecting anti - Leishmania donovani antibodies in serum samples from suspected patient  (children under 8 years ) with Visceral Leishmaniasis V.L..  Sera from 73 V.L. , 60 Healthy controls, and 57   patient with other parasitic diseases other than V.L. (Amoebiasis, Giardiasis , Toxoplasmosis, Schistosomiasis , Hydatidosis, Ascariasis , Lupus Erythromatosus , Viral Hepatitis, and Cutaneous Leishmaniasis) were examined. Anti Leishmania donovani antibodies detected  in 71 out of 73  suspected Visceral Leishmaniasis . Data of this study showed that infection in male group was more than female group. Result of this study revealed a high prevalence of patients with V.L. infection at the age range  1 - 2.9 and 3 - 4.9 years when compared with other range groups (P>0.01). A cross – reaction was found with sera Lupus Erythromatosus, No cross - reaction was found with sera from other disease . Sensitivity was determined as   97.3 %, and Specificity was found to be 95.7% . Positive and Negative  the  Values   Predictive of test were   96% and  97.4% Dot .ELISA is simpler, Serodiagnosis Rapid, sensitive and specific, Its use in respectively. Seeing that   of V.L.   can be recommended.