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ijs-6225
Qualitative and Quantitative Molecular Analysis of Epstein-Barr Virus in Iraqi Patients with Relapsing-Remitting Multiple Sclerosis
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    Multiple sclerosis (MS) is a neuro-inflammatory disorder in which the Epstein-Barr virus (EBV) is proposed to have a pathogenic role. Therefore, a case-control study was performed (93 patients with relapsing-remitting MS and 113 healthy controls (HC) to analyze the prevalence and viral load of EBV infection using real time-polymerase chain reaction. Prevalence of EBV infection was lower in patients compared to HC but the difference was not significant (12.9 vs. 21.2%; probability [p] = 0.187). EBV-positive MS cases were more common in females than in males (83.3 vs. 16.7%), while an opposite distribution was observed in HC (37.5 vs. 62.5%), and the difference was significant (p = 0.041). Blood group O frequency was higher in EBV-positive patients compared to the corresponding HC but the difference was not significant (33.3 vs. 20.8%; p = 0.443). EBV-positive MS cases showed similar frequency in the two groups of the expanded disability status scale (EDSS: < 3.0 and ≥ 3.0; 50% each). EBV load was significantly elevated in EBV-positive MS cases compared to EBV-positive HC (94.6 ± 84.2 vs. 17.0 ± 16.3 DNA copy/100 cells; p = 0.009). When EBV-positive MS cases were classified by gender, EDSS groups or ABO blood groups, there were no significant differences between EBV loads in each stratum. However, a significant correlation between EBV load and EDSS was found (correlation coefficient = 0.620; p = 0.031). In conclusion, the prevalence of EBV infection showed no significant differences between MS patients and HC, while EBV load was significantly higher in patients.

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Publication Date
Sun Oct 22 2023
Journal Name
Iraqi Journal Of Science
Detection of Human Leukocyte Antigen and Celiac Disease Auto Antibodies in serum of Patients with Multiple Sclerosis
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To determine the important pathogenic role of celiac disease in triggering several
autoimmune disease, thirty patients with Multiple Sclerosis of ages (22-55) years
have been investigated and compared with 25 healthy individuals. All the studied
groups were carried out to measure anti-tissue transglutaminase antibodies IgA IgG
by ELISA test, anti-reticulin antibodies IgA and IgG, and anti-endomysial
antibodies IgA and IgG by IFAT. There was a significant elevation in the
concentration of anti-tissue transglutaminase antibodies IgA and IgG compared to
control groups (P≤0.05), there was 4(13.33%) positive results for anti-reticulin
antibodies IgA and IgG , 3(10%) positive results for anti-endomysial antibodies

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Publication Date
Mon Jun 11 2018
Journal Name
Internationaljournalofresearchinpharmaceuticalsciences
Treatment satisfaction with disease-modifying therapy in relapsing-remit-tingmultiple sclerosis in Iraq
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Multiplesclerosis(MS)isachronic,inflammatory,immune-mediateddiseaseof the central nervous system (CNS). More than 2 million people worldwidehave MS. The goal of the present study was to compare Iraqi patients' treat-ment satisfaction with three different disease-modifying therapies (DMTs),administeredorally,subcutaneously,andbyslowinfusion;namely,fin-golimod, interferon beta-1b (IFNβ-1b), and natalizumab, respectively. Aswell as to assess the individual differences among these therapies about theireffectiveness, convenience and global satisfaction also to assess the role ofcertain predictors on treatment satisfaction. Patient satisfaction with medi-cation assessed by the Treatment Satisfaction Questi

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Publication Date
Sun Apr 01 2007
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Pain In Patients With Multipe Sclerosis
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Background: pain is frequently listed among initial symptoms of MS or an occurring in the course of the disease.
Patients and Methods: one hundred thirty MS patients diagnosed according to Macdonald's criteria compared with 115 matched age and sex control were interviewed about pain Neuropathic, somatic and headache.
Results: Neuropathic pain was significantly higher in MS than control group, while LLD and ARP were of no significance difference between two groups. For somatic pain there was significant increase in MS.
Conclusion: pain is common in MS especially LLD. There is no significant difference between pain subtypes and duration of disease and FS, EDSS.

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Publication Date
Mon Apr 01 2013
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Molecular Study of Human Mammary Tumor Virus in Iraqi Women with Breast Cancer
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Background: Earlier reports related the presence of Mouse Mammary Tumor Virus -like gene sequences to human breast carcinoma. Mouse Mammary Tumor Virus -like gene is a retrovirus, namely, a virus containing reverse transcriptase which transcript its RNA to DNA in a process that enables genetic material from the retrovirus to become a part of the genes of an infected cell permanently. The virus that found in women was designated as Human Mammary Tumor Virus by the authors, who have investigated the presence of Human Mammary Tumor Virus sequences in a many human breast tissues and in many countries.

Objectives: Detect HMTV genome in Iraqi women of breast cancer.

Patients and Methods

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Publication Date
Fri Jul 01 2022
Journal Name
Iraqi Journal Of Science
Molecular Analysis of Y Chromosome Microdeletions in Oligozoospermic Iraqi Patients
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This study was designed to determine the correlation between Y chromosome azoospermia factor (AZF) subregions microdeletions and oligozoospermia in infertile men. Subjects included 50 infertile men with oligozoospermia who had been referred to the Fertility Center and infertility treatment in Kamal Al-Samarrai Hospital\Baghdad health office-Iraq. DNA was extracted from blood samples. Polymerase chain reaction (PCR) amplification of 3 loci spanning the AZFa, AZFb and AZFc subregions of the Y chromosome using sY84, sY127 and sY254 and were performed. The frequency of deletions involving AZFa subregion of the Y-chromosome was found in twelve of the patients (24%) in oligozoospermic infertile Iraqi men. While the other subregion (AZFb and AZ

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Publication Date
Thu Jun 29 2023
Journal Name
Farmacia
CORRELATION BETWEEN INTEGRIN ALPHA-4 GENE POLYMORPHISMS AND FAILURE TO RESPOND TO NATALIZUMAB THERAPY IN IRAQI MULTIPLE SCLEROSIS PATIENTS
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Publication Date
Sat Feb 01 2020
Journal Name
Meta Gene
Interleukin-2 serum level, genetic polymorphism (rs2069763), anti-rubella antibody and risk of multiple sclerosis among Iraqi patients
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Background: Multiple sclerosis (MS) is a chronic neurodegenerative autoimmune disease mediated by autoreactive T cells against myelin-basic proteins. Cytokines are suggested to play a role in the etiopathogenesis of the disease. Among these cytokines is interleukin-2 (IL-2). Aim of the study: To investigate the association between IL2+166 G/T single nucleotide polymorphism (SNP: rs2069763) and MS in Iraqi patients. Serum level of IL-2 was also detected. Anti-rubella IgG antibody was further determined in the sera of patients. Patients and methods: Eighty MS patients (28 males and 52 females; age mean ± SD: 39.2 ± 16.1 years) and 80 healthy control matched patients for age (32.15 ± 16.13 years) and gender (28 males and 52 females) were en

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Publication Date
Thu Jun 30 2022
Journal Name
Iraqi Journal Of Science
Study of ABO System and Multiple Sclerosis disease in Iraq
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      Multiple sclerosis (MS) is one of an autoimmune condition with uncertain etiopathology. According to new data, ABO system had played a role in the development and understanding numerous diseases. Lower level of 25-hydroxy vitamin D3 (25-OHD3) is considered as a risk factor for MS. The aims of this study is to identify the role of blood group distribution on the levels of parathyroid hormone (PTH), 25-OHD3, total calcium, inorganic phosphorus and total magnesium on MS patients. Additionally, we assessed the relation between Expanded Disability Status Scale (EDSS) and study parameters in patients. The Study included 107 patients with MS were distributed in to four groups according to their blood group (A, B, AB, and O).  Additi

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Publication Date
Sun Mar 31 2019
Journal Name
Journal Of Pure And Applied Microbiology
Determination of Level of 25-Hydroxy Vitamin D3 in Patients with Multiple Sclerosis and Its Effect on Disease Activity
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Publication Date
Thu Sep 29 2016
Journal Name
Enzyme Research
Molecular Analysis of CYP21A2 Gene Mutations among Iraqi Patients with Congenital Adrenal Hyperplasia
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Congenital adrenal hyperplasia is a group of autosomal recessive disorders. The most frequent one is 21-hydroxylase deficiency. Analyzing CYP21A2 gene mutations was so far not reported in Iraq. This work aims to analyze the spectrum and frequency of CYP21A2 mutations among Iraqi CAH patients. Sixty-two children were recruited from the Pediatric Endocrine Consultation Clinic, Children Welfare Teaching Hospital, Baghdad, Iraq, from September 2014 till June 2015. Their ages ranged between one day and 15 years. They presented with salt wasting, simple virilization, or pseudoprecocious puberty. Cytogenetic study was performed for cases with ambiguous genitalia. Molecular analysis of

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