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Molecular detection of Chlamydia trachomatis infection among males with abnormal semen

Many studies revealed that Chlamydia trachomatis is the most prevalent bacteria “that cause sexually transmitted infections in the world.. Most of these infections are asymptomatic and there is a remarkable relationship between CT infections and male infertility. Therefore, “the present study is established to determine the effect of Chlamydia trachomatis infection on seminal fluid quality in males suffering from infertility compared with fertile males.. “Semen samples were collected from 63 infertile male and 13 fertile male as control group, attending the clinical laboratory for routine seminal fluid analysis. Seminal fluid was analyzed according to World Health Organization guidelines, the whole genomic DNA was extracted for molecular study. Real time PCR technique was used to specifically detect the presence of CT DNA in semen samples.”

  1. trachomatis was demonstrated in the semen samples of 11 (17.4%) infertile male and all control samples were negative. Infertile, infected males had semen samples that showed statistically significant differences in the mean of total sperm count, motility and morphology as compared with fertile uninfected control samples. These differences indicate that chlamydial infection of genital tract could negatively influence the quality of seminal fluid.”

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Publication Date
Fri Jun 24 2022
Journal Name
Iraqi Journal Of Science
Molecular Characterization Aminoglycosids Resistance Pseudomonas aeruginosa

Resistance to aminoglycosids is a great problem to therapeutics. Aminoglycoside acetyltransferase producing Pseudomonas aeruginosa have been reported to be important cause of nosocomial infections. The purpose of this study was to determine the occurrence of aminoglycoside acetyltransferase. A total of 200 clinical and environmental samples were collected over period of five months. The P. aeruginosa isolates were confirm their identification, antibiotic susceptibility profile according to vitek2 compact system. The isolates were subjected to polymerase chain reaction (PCR) assays with specific primers for aac (6')-I, aac (6')-Ib, aac (3')-I . Only 32 (16.%) P. aeruginosa isolates were recovered from the samples. in present investigation

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Publication Date
Thu Dec 29 2016
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Isolation and Diagnosis of Aerobic Bacteria from Patients with Respiratory Tract Infection and Partial Characterization of Their Plasmid Profile

   In this study, the bacteria from sputum specimens of patients with respiratory tract infections were isolated in   IbnSina Teaching Hospital, Mosul city, Iraq.  The bacteria  were subjected to phenotypic and biochemical tests necessary for identification. Twenty five isolates of six different bacterial species were obtained, they are : Staphylococcus aureus, Streptococcus pneumonia, Moraxella cattarhalis, Escherichia coli, Klebsiella pnuemoniae and Pseudomona saeruginosa with ratios (64%, 12%, 8%, 8%, 4% and 4% ) respectively. The sensitivity and resistance of these isolates to 12 antibiotic were studied, where the Gentamycin appear to be more effective on most of the isolates while all the isolates showe

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Publication Date
Sun Jul 31 2022
Journal Name
Iraqi Journal Of Science
Potential Role of TLR3 and RIG-I Genes Expression in Surviving COVID-19 Patients with Different Severity of Infection

      Immunological genes, including TLR3 and RIG-I, have recently been established to have linked to predisposition to coronavirus disease 2019 (COVID-19) and its severity. The purpose of this case-control study (100 recovered COVID 19 cases and 100 healthy individuals) was to determine the role of gender, age, TLR3 and RIG-I genes in COVID-19 aggressiveness. TLR3 and RIG-I gene expression was detected using a quantitative real-time polymerase chain reaction (qRT-PCR). COVID-19 infection intensity increased with age and no statistical difference between males and females (p>0.05) was found. TLR3 and RIG-I gene expression levels were higher in patients compared to hea

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Publication Date
Tue Jan 01 2019
Journal Name
Malaysian Journal Of Biochemistry And Molecular Biology
Scopus
Publication Date
Tue Feb 05 2019
Journal Name
Journal Of The College Of Education For Women
Altruism and its relation with self control among university students

Altruism is one of good qualities of human being expressing his highness and triumph. Self control has A signifies in a lot of psychological, educational, Social, ethical and healing fields Where the higher self control relates with high degrees of carefulness accuracy, problem solving, choice. judgment and decision making. the present study aimos at measuring altruism among university students , self control among them , recognizing the statistically differences significance on self control among university students according to gender variable and recognizing the correlation between attruism and self control among university students . the sample consists of the basic application one (200) students (100) males and (100) males that had

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Publication Date
Tue Apr 01 2008
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Trichomoniasis Among Females With Vaginal Discharge in Baghdad Medical City

Background:
Trichomonas vaginalis is a pear shaped parasite, with a short undulating membrane, lined with a flagellum and four anterior flagella. It is one of the most
common organisms causing infection in the female genital tract, which is normally limited to vulva, vagina and cervix. It doesn't usually extend to the uterus. The
mucosal surface may be tender, inflamed and covered with a frothy yellow or cream colored discharge.
Aim:
To evaluate the infection rate of Trichomonas vaginalis among females complaining of vaginal discharge with or without pruritis vulvae, and to isolate the
parasite by different laboratory methods, such as wet mount method, culture on special media and staining by sp

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Publication Date
Sun Apr 01 2018
Journal Name
Journal Of Educational And Psychological Researches
Personal Efficacy And Its Relationship With Mindfulness Among University Students

The study seeks to examine the level of personal efficacy and its relation to mental alertness among university students. Besides, the statistically significant differences in regard of students' gender, and the correlation between male and female. To do this, the researcher adopted two scales: one to measure the personal efficacy which was made up by (abed al-jabaar, 2010) included (26) items, and the other to measure the mental alertness that designed by (abed Allah, 2012) included (36) items. A total of (120) student were selected randomly from three-different colleges at the Al-Mustansiriyah University for the academic year 2016-2017. The findings revealed there are no significant differences among students in regard of the personal

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Publication Date
Sun Oct 01 2017
Journal Name
Journal Of Educational And Psychological Researches
Existential frustration and its relation with recrimination among university students

The study aims to identify the level of existential frustration and the level of recrimination among the students of universities, identify the statistical differences between the existential frustration and recrimination based on gender, and finally, identify the correlation between the existential frustration and recrimination. To do this, the researcher adopted the existential frustration scale of ( al-saaedi, 2009) that consisted of (43) item, he also adopted the recrimination scale of ( al-zugeibi,2008) which composed of (31) item. The total sample was (120) male and female student were chosen randomly from four colleges within the university of Baghdad for the academic year ( 2015-2016). The results revealed that the targeted sampl

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Publication Date
Sun Apr 01 2018
Journal Name
Journal Of Educational And Psychological Researches
Relationship sensitivity to stress with positive mood Among Educational Counselors

The study aimed to examine the sensitivity to stress and its relation to positive mood among educational supervisors. The researcher used the descriptive approach as more an appropriate method for the current study. The sample is composed of (200) educational supervisor. To collect study data, two scales have been used: one scale to measure the sensitivity to stress and the other to measure the positive mood. The results indicated that a high level of sensitivity to stress with low level of positive mood among educational supervisors, sensitivity to stress showed significant differences among the sample regarding to major. There is no correlation between sensitivity to stress and positive mood, and finally, there are no significant diffe

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Publication Date
Thu May 30 2024
Journal Name
Iraqi Journal Of Science
Lipid Profile Parameters and Adipokines among Adolescents Infected with Toxoplasmosis

The lipid profile and adipokines of an adolescent may be affected by some parasite infections. Recently, it has been discovered that these parasites are connected to body mass index (BMI), lipids and adipokines. The current study, therefore, aimed to specify how Toxoplasma gondii (T. gondii) affect BMI, lipid profile and adipokines. This study was conducted in Al Madain hospital, Baghdad from October to December 2022. An ELISA test was performed to examine the anti-T. gondii IgG and IgM for a group of adolescents attending the hospital. Based on this examination ninety adolescents were chosen to be involved in the study. They were separated in to two groups: individuals who tested positive for the parasite (n=45) and those who teste

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