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Detection of Efflux Pumps Gene and Relation with Antibiotics Resistance in Uropathogenic Escherichia Coli (UPEC) Isolated from Patients with Cystitis
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Background: In recent years, the multidrug-resistant (MDR) Escherichia coli has increased in urinary tract infections (UTIs). One of the highly distributed chromosomally encoded traits of resistance is efflux pump. The current study aimed to investigate the most common members of 5 classes of efflux pumps among uropathogenic E. coil isolates.

Methodology: E. coli isolates were isolated using conventional bacteriology tests   and confirmed by the uidA gene. An antibiotic susceptibility test has been done against 25 antibiotics using disc diffusion method. Efflux pump genes have been examined via polymerase chain reaction. Biofilm formation was investigated by a 96-well polystyrene microtiter plate.

Results: In total, 50 E. coli isolates were collected from UTIs. All isolates were resistant to amoxicillin, ceftazidime, and cefotaxime, while all isolates were sensitive to imipenem.  Also, 19 (38%) E. coli isolates were non-MDR, while 31 (62%) were MDR.  The frequency rates of different efflux pump genes ranged from 66% to 100%. The biofilm formation results indicated that 98% of isolates were biofilm former while 2% were non-biofilm. In total, 60% was weak biofilm, 24% was moderate, and 14% was strong biofilm former.

Conclusion: The current study concludes that all efflux pumps may be highly associated with resistance to amoxicillin, cefotaxime, and ceftazidime and moderately associated with cefepime, ceftriaxone, and cefixime and may be unrelated to the resistance of other studied antibiotics or the concentration of these antibiotics inadequate to induce the expression of these pumps. Additionally, biofilm formation was highly related to the presence of study pumps.

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Publication Date
Sat Jul 01 2017
Journal Name
Journal Of Nano Research
Electroluminescence Devices from Quantum Dots with TPD Polymer White Light Generation
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Quantum dots of CdSe, CdS and ZnS QDs were prepared by chemical reaction and used to fabricate organic quantum dot hybrid junction device. QD-LEDs were fabricated using ITO/TPD: PMMA/CdSe/Al, ITO/TPD: PMMA/CdS/Al and ITO/TPD: PMMA/ZnS/Al QDs devices which synthesized by phase segregation method. The hybrid white light emitting devices consists, of two-layers deposited successively on the ITO glass substrate; the first layer was of N, N’-bis (3-methylphenyl)-N, N’-bis (phenyl) benzidine (TPD) polymer mixed with polymethyl methacrylate (PMMA) polymers in ratio 1:1, while the second layer was 0.5wt% from each type of the (CdSe, CdS and ZnS) QDs for each device.The optical properties of QDs were characterized by UV-Vis. and photolum

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Publication Date
Thu Feb 01 2018
Journal Name
Human Immunology
Cytokine gene polymorphisms in Iraqi Arabs
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Publication Date
Tue Jul 06 2010
Journal Name
Journal Of College Of Education
Association Between lnsulin Resistance And Diabetes Mellitus
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Background: Obesity and diabetes mellitus are the common health problems,and obesity is common cause of the insulin resistance. Aim of studv: Aim of the study is to find any correlation between obesity (insulin resistance) and type I diabetes in children. Patients and methods: This study included (40) children with type I diabetes, in addition to (40) children as control.The age of all studied groups ranged from (8-18) years.This study was attemted from Ibn AlBalady Hospital during from 20 August to 9 Novembar,2008. The subjects wrer divided into (4) groups according to their BMI:- * Obese children,diabetes,n=2O,BMI>30. * Non obese children, diabetes, n=20,BMI<25. Obese children, non diabetes, n=20,BMI>30. * Non obese children,non diabetes

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Publication Date
Mon May 22 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Burn wound Infections and Antibacterial Resistance to Antibiotic in Baghdad, Hospitals
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 A total of 291 specimens were obtained from hospitalized and out patients. They were 10-70 years old, males and females suffering from burn wound infections, during the period29/4/2007–29/9/2007. InAl-Karekh-surgical-hospital&Al-Kadhemiatechinghospital   The most common single isolated pathogen was Pseudomonas aeruginosa %38.6 , Escherichia coli %23.7 Klebsiella spp. %12.2 , Staphylococcus spp. %2.19 and Proteas spp. %1.32.  As mixed isolates  Pseudomonas aeruginosa and  Escherichia coli %12.3, Pseudomonas aeruginosa and Klebsiella spp. %0.9 were found. The study isolats were highly resistant to chloromphenicol %10 Tobromycin %97.43, Gentamycin %87.14, Garamycin %83, Pipracellin %83, Penicillin %

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Publication Date
Fri Dec 30 2022
Journal Name
Iraqi Journal Of Science
Study of Possible Changes in Lipid Profiles between Premenopausal and Postmenopausal Women with Hyperthyroidism and Others with Hypothyroidism
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      Menopause is the lack of menstrual cycle for at least six months. Due to hormonal changes, the alternation of lipid profile as a risk factor related to heart disease increases during menopause . One hundred twenty Iraqi women, aged between 40-65 years, were selected for this study. They were divided into two groups: 60 premenopausal and 60 postmenopausal women, and then each group was further divided into two subgroups: 20 women with hyperthyroidism and 20 with hypothyroidism, as well as 20 healthy women as control group. Blood samples were collected to estimate  hormonal parameter by enzyme linked immunosorbent assay (ELISA) and lipid profile using enzymatic technique. The results showed that alterations in lipid profile inc

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Publication Date
Mon Apr 23 2018
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Comparative Analysis of Various Techniques for Giardia lamblia Detection and Association with E coil and Shigella Among Children Attending AL-Imamin AL-Kadhimin Medical City
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 Diarrhea is an important public health problem worldwide, several causes associated with diarrhea especially in population live under poverty and unsafe water use. Different methods are available and use in diagnosis. This study was carried out to compare of various techniques for Giardia lamblia detection and study the association with  E coil and Shigella in patients with diarrhea.  A total of 100 children with diarrhoea were enrolled into the study, 57 were males and 43 were females, aged from 2 months -16 years were attendant to AL-Imamin AL-Kadhimin Medical City, during the period from May 2014 to February 2015.  Stool samples were collected and analysed for Giardia lamblia

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Publication Date
Thu Aug 15 2019
Journal Name
Scholarspace
Folklore as Resistance in Postcolonial Narratives and Cultural Practices: Hawaiian, African American, and Iraqi
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Colonialism radically transformed the cultures of colonized peoples, often rupturing Indigenous traditions and folklore. Whether creating colonial discourse, promoting orientalist literature, advocating western educational institutions, or through biased media representations, imperial powers systematically oppressed Indigenous and Native peoples. Subjugated communities, however, created, and still form postcolonial discourse from their knowledge systems. This discourse insists on Indigenous and Native culture as central to Indigenous and Native peoples identity. This study examines the postcolonial literature of three groups: Kānaka Maoli, African Americans, and Iraqis. The scope of this dissertation scrutinizes how folklore is employed

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Publication Date
Fri Sep 01 2023
Journal Name
The Medical Journal Of Malaysia
Serum interleukin-40: an innovative diagnostic biomarker for patients with systemic lupus erythematosus
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Publication Date
Sun Sep 01 2024
Journal Name
International Immunopharmacology
Evaluating treatment effect on interferon-alpha in female patients with systemic lupus erythematosus: a case-control study
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Evaluating treatment effect on interferon-alpha in female patients with systemic lupus erythematosus: a case-control study

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Publication Date
Sun Jun 02 2019
Journal Name
Baghdad Science Journal
Effect of D-Mannose on Gene Expression of Neuraminidase Produced from Different Clinical Isolates of Pseudomonas aeruginosa
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The present work aimed to investigate the neuraminidase (nan1) gene expression in 32 different clinical isolates of Pseudomonas aeruginosa to explore the role of the enzyme in different types of infection and might give a better understanding of host cell-pathogens interaction. In addition, the effect of monosaccharide D-mannose on neuraminidase gene expression in eight isolates was studied by utilizing a reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The results demonstrated that the highest expression of nan1 gene was in otitis samples (208,913.81) which were significantly higher than that from other infections (P < 0.01). While, the concentrations of gene copies obtained from urin

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