This study included the isolation and identification of Citrobacter freundii from 220 samples collected from inpatients and outpatients suffering from urinary tract infection (UTI) and identified at the laboratory of the General Samarra Hospital in Samarra City, Iraq. The study was conducted to investigate some of the virulence factors produced by C. freundii. The results showed that 67 isolates were belonging to the C. freundii, with a rate of 30.45%. Twenty eight samples were from inpatients (41.8%) and 39 samples were from outpatients. The bacterial identification was based on cultural and biochemical tests and confirmed by using VITEK2 compact system. Virulence factor results showed that all isolates were not blood hydrolyzing whereas they produced protease. Seven isolates (10.4%) produced biofilm, five from inpatients and two from outpatients, at rates of 17.8% and 5.1%, respectively. The results showed that 17 (25.4%) of the pathogenic isolates were β-lactamase producers, as determined by the iodometric method, twelve of them (17.9%) were from inpatients and 5 (7.5%) from outpatients. Four isolates of C. freundii produced Extended Spectrum Beta-lactamase (ESβL) enzymes, three from inpatients and one from outpatients, with ratios of 4.5% and 1.4%, respectively. Also, the via B gene, which is responsible for virulence factors, was investigated using PCR. The results showed that 12 isolates from inpatients and 4 isolates from outpatients were harboring this gene. The antimicrobial susceptibility testing by Kirby-Bauer’s method showed that all isolates that produced β-lactamase were resistant to antibiotics.
Isolation and identification of bacterial isolates were carried out according to the morphology and biochemical characteristics on one hundred and twenty stool specimens collected from children under five years old via using biochemical tests and Api 20E compact system for further confirmation. Bacterial isolates were distributed as (34.48, 20.68, 5.17,0.86) % for Escherichia coli, Salmonella typhi ,Enterobacter aerogenos, Citrobacter freundii and Hafnia alvei respectively and 9.48 % for each Proteus mirabilis, Pseudomonas aeruginosa and Klebsiella Pneumonia. As well as, 2.58% for both Shigella sonnei and Serratia marcescens. Antibiotic susceptibility test for 116 bacterial isolates was performed towards 20 antibiotics types using disk d
... Show MorePseudomonas putidaPST-1 isolate isolated from soil of plant root was used for high production of indole acetic acid. Indole acetic acid (IAA) production is a major property of rhizosphere bacteria that stimulate and facilitate plant growth. Optimization of indole acetic acid production was carried out at different cultural conditions of pH temperature, incubation period, and the amount of inoculum of bacteria. The best chemical medium for high IAA production (82 Mg/ml) was Luria-Bertani broth medium consisted of 1.2gm tryptophan and 10gm peptone in their components, while the cheese whey medium was the best natural medium for IAA production was (66 Mg/ml). IAA production byPseudomonas putida PST-1 was optimized by studying some factors t
... Show MoreThe ground state proton momentum distributions (PMD) and elastic charge form
factors for some odd 1f 2p shell nuclei, such as , , 59 63Co Cu and Cu 65 have been
studied using the Coherent Density Fluctuation Model and formulated by means of
the fluctuation function (weight function) ( ) .
2
f x The fluctuation function has been
connected to the charge density distribution of the nuclei and determined from the
theory and experiment result. The feature of the long-tail behavior at high
momentum region of the PMD has been calculated by both the theoretical and
experimental fluctuation functions. It is found that the inclusion of the quadrupole
form factors ( ) 2 F q C in all nuclei under study, which are de
Background: Soft Laser has been advantageous in medical applications and is widely used in clinical practice. It is applied because it doesn’t cause the significant thermal effects or tissue hurt when irradiated. The blood response to low power laser radiation provides information about processes of laser radiation interaction with live creatures. Objective: The aim of the current work was to evaluate the laser-induced changes of in vitro erythrocyte sedimentation rate (ESR), mean corpuscular volume (MCV), and mean corpuscular hemoglobin concentration (MCHC) in patients with breast cancer by irradiating a human blood sample using a green laser and comparing its effects before and after irradiation with the same power density (100mW/c
... Show MoreAbstract Objective: The study aimed to assess the factors contributes of patient with bladder cancer and to find out the relationship between the factors of bladder cancer with certain variable. Methodology: A descriptive study to assessment of factors that contribute to bladder cancer that was carried out Al-Karama teaching hospital, Al-Kendy teaching hospital, Specialty Surgery teaching hospital and Al-Yarmok teaching hospital for the period of November 2003 to August 2004. A purposive (non-probability) sample of (100) patients with bladder cancer. An assessment from was constructed for the purpose of the st
This study aimed for isolation and identification of Candida glabrata and identifying some virulence factors. The distribution of patients with candidemia thrush showed that the age group 50-65 years old recorded the highest incidence of candidiasis in female and male with leukemia by 50% and 37.9 % respectively compared to the lowest incidence of candidiasis in the age group under 17 years old in female and male 8.8% and 13.5%, respectively. While the age group between 5-8 years was high, reaching 18 cases of oral candidiasis and 42% of children with leukemia compared with the age group, which was the least, that reached 9 cases, 21%. The highest incidence of C. glabrata was 59 isolates of females and males with leukemia, while C. kefyer w
... Show MoreAim of the present study is Identification of specific gene for GPCR using specific primers .and identification of difference in PCR analysis in patients with heart thrombosis and compared with healthy, Sequencing of PCR product regarding GPCR compared for all three subject, Identification the similarity of human GPCR with local strain of yeast fifty healthy control and fifty patients with thrombosis which diagnosed medically with cardiac specific troponin t, troponin 1 levels and electro myocardiogram ECG. The aged for all subjects ranged (39-75) years patients were lying in cardiac care unit at Ibn- al- Nafees teaching hospital and Sheikh Zayed teaching hospital. Genomic DNA of whole blood was extracted from buffy coat and cell cu
... Show MoreDirect determination of trace metals Zn, Mn, Cu and Co were performed in serum
blood samples of two groups diabetic patient type 2 and non diabetes by ICP spectrometric
method. Results show the low levels of these elements Zn, Mn and Co while high level of Cu
detected compared with non diabetes according to these results good evidence can be made to
control these levels through a special diet containing these metals.