Pseudomonas putidaPST-1 isolate isolated from soil of plant root was used for high production of indole acetic acid. Indole acetic acid (IAA) production is a major property of rhizosphere bacteria that stimulate and facilitate plant growth. Optimization of indole acetic acid production was carried out at different cultural conditions of pH temperature, incubation period, and the amount of inoculum of bacteria. The best chemical medium for high IAA production (82 Mg/ml) was Luria-Bertani broth medium consisted of 1.2gm tryptophan and 10gm peptone in their components, while the cheese whey medium was the best natural medium for IAA production was (66 Mg/ml). IAA production byPseudomonas putida PST-1 was optimized by studying some factors the results revealed that the maximum IAA value was obtained when the isolate cultivated in Luria-Bertani broth medium supplemented by tryptophan 1.2gm / 1L and peptone 10gm/1L, adjusted at pH 7 and incubated at 30 c for 4 days with the viable count of bacteria was (19,8 × 103). These results suggest that IAA producingPseudomonas putida PST-1 could be promising candidate for utilization in growth improvement of plants of economic and agricultural value.
This research had been conducted to investigate response of two Antirrhinum majus L. varieties, tall and dwarf variety to foliar application of Indole 3-acetic acid and Benzyladenin at (0, 60, 120, 180, 240 ppm), through plant height, fresh weight, dry weight of shoot, length of inflorescence (raceme), number of flowering buds per raceme and chlorophyll content in leaves at complete flowering stage.
Results of treatment of the tall variety with IAA and BA showed that IAA had the highest significant increase in plant height, while BA had the highest significant increase in chlorophyll content .Increasing concentrations of both IAA and BA resulted a significant increase in all growth parameters, this was obvious in floweri
... Show MoreThis study on the plant of Ain –AL Bason Catharanthus roseous showed the ability of callus cells that is produced by In Vitro culture technique and transformed to the accumulated media (MS 40gm/L sucrose ,2gm/L IAA Indole acetic acid , 0.5gm/L Tryptophan) to produce Vinblastine and Vincristine compounds. Extraction, purification and quantitive determination of Vinblastine and Vincristine compounds using High performance liquid chromatography technique (HPLC)were carried out. The results showed that the highest concentration of Vinblastine and Vincristine compounds were ( 4.653,12.5 (ppm /0.5 dry Wight respectively from transformed callus cells from MS 40 gm /L sucrose , 2 gm / L NAA Naphthaline acetic acid .
Two new ligands Na2[ H3B (BDIA)].0.05H2O (L1)(BDIA = 1-Boranyl-2,3-
Dihydro-1H-Indol-3-yl)]Acetic Acid and Na3[H2B(BDIA)2].0.3H2O.0.3CH3Ph (L2)
were synthesized by reaction of NaBH4 with indole -3- acetic acid (IAA) . The
coordination properties of ligands were studied with Co(II) , Ni(II) , Cu(II) and
Pt(IV) ions. Characterization and structural aspects of the prepared compounds were
elucidated by 1HNMR, FTIR electronic spectra, magnetic susceptibility, elemental
and metal analysis, thermal analysis (TG & DTG) and conductivity measurements.
The obtained data for metal complexes suggested square planar geometry for
copper complexes, octahedral geometry for nickel and platinium complexes and
tetrahedral geom
Production and characterization of methionine γ- lyase from Pseudomonas putida and its effect on cancer cell lines
Some microorganisms can produce nanocellulose, which is known as bacterial nanocellulose (BNC); the most active bacterial producer is acetic acid bacteria (AAB), which is a gram-negative, motile and obligate aerobic belongs to the family Acetobacteraceae. Bacterial nanocellulose has excellent attention in medical (surgical domain), industrial and pharmaceutical fields because of its flexible properties, characteristics and advantages. So, in this study, the AAB (5AC) isolate was isolated from apple vinegar. The production of BNC was performed by using a natural medium called palm dates liquid medium, the produced bacterial Cellulose was purified by using the sodium hydroxide method; it was observed that the wet weight of the BNC was a
... Show MoreThis study was done to determine the effect of watery and alcoholic extracts of Elettaria cardamomum on growth of bacteria isolated from Otitis media Infections which are : Pseudomonas aeruginosa , Staphylococcus aureus and Klebsilla spp. The biochemical analysis revealed that watery extracts of Elettaria cardamomum contains: Glycosides, Alkaloids, Saponines, Tannins, and Volatile oils, ( This compound is Soluble in water ) , while the alcoholic extracts contains : (in addition to the above mentioned compounds ) Phenols , Resins , Flavonids and Coumarins. ( This compound is Soluble in organic solutions ). To study the e
... Show MoreRhizobium bacteria was isolated from the root nodules of Medicago sativa plants and, based on morphological and some biochemical properties, it was characterized as Sinorhizobium meliloti. We studied the ability of this isolate, as well as that of Agrobacterium rhizogenes R1601, to produce the auxin indole acetic acid (IAA). For purposes of control, both isolates, in the absence of tryptophan-L, were similarly tested. The identification of IAA was achieved by checking the colour reactions with Salkowski’s reagent. Low amounts (23, 69 and 26,77 µɡ/ml) of IAA were produced by S.meliloti and A.rhizogenes after 24 and 72 hours of incubation, respectively. S.meliloti was
... Show MoreFoliar application and seed soaking has been used as a means of supplying supplemental doses of nutrients, plant hormones, stimulants, and organic components. the effects of these applications have included yield increases, and improved drought tolerance, and enhanced crop quality, so A field experiment was carried out during spring seasons in 2019 and 2020 for styding Seed soaking and Foliar Application of Ascorbic acid, Citric acid and Humic acid on Growth, Yield and Active Components IN Maize. Randomized complete block design in split plots arrangement was used with three replicates. Main-plots were for seeds soaking with ascorbic, citric (100 mg l-1) frequently and humic at (1 ml l-1). Sub-plots were for vegetative parts nutrition with
... Show MoreBy- products of corn starch industry were used to prepare media for propagation the lactic acid bacteria as a natural auxotroph. The by- products used were the corn steep water (S) and gluten extract (G) after a proper treatment to get them ready for media preparation. The results showed that it was possible to replace the peptone and meat extract by gluten extract in MRS medium. The growth was approximately similar to that obtained in standard MRS media. Corn steep water (S) was used as well and the growth enhanced by including Tween – 80 at 1% level. The later media named MZ, which was superior for growing standard and local strains and starters. The MZ medium modified by adding acetate and glacial acetic acid similarly to
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