Alternation of bacterial antioxidant defense pathways might affect susceptibility to antibiotics in dual ways. Using a relatively simple model based on wild-type and oxyR Escherichia coli mature biofilms, their counterpart planktonic cultures and exponentially growing planktonic cultures, we explored the role of OxyR-mediated metabolism alternations in modulation of susceptibility to antibiotics ciprofloxacin and cefotaxime. All three types of cultures were placed in fresh medium,1 h after antibiotics were added and incubation continued further for 2 h. Killing rates of antibiotics were determined, biofilm eradication using crystal violet assay was estimated, expression of rpoS, katG, sulA genes as well as HPI and HPII catalase activity were measured. Biofilms of both strains were more recalcitrant to ciprofloxacin and cefotaxime at all tested concentrations compared to exponentially growing planktonic cultures. In oxyR biofilms killing rate of ciprofloxacin was lower, and killing rate of cefotaxime was higher compared to the parental strain. Compared to biofilms, wild-type biofilm counterpart planktonic cultures showed higher tolerance to low doses of ciprofloxacin, while oxyR plankton demonstrated higher tolerance to cefotaxime. Higher recalcitrance of oxyR biofilms to ciprofloxacin may be caused by an increase in persister cells under conditions of enhanced oxidative stress and activated SOS response.
An experiment is conducted to determine the effect of magnetic water on the production performance and blood characteristics of common quail, Coturnix coturnix (Linnaeus, 1758). The experimental treatments included T1 = control treatment, T2 = drinking water magnetization at1000 gauss, T3 = drinking water magnetization at 1500 gauss, T4 = diet sprayed with magnetized water 1000 gauss, T5 = diet sprayed with magnetized water 1500 gauss, each treatment with three replicates. The results showed significant superiority of control group in the body weight, weight gain and feed intake compared with treated groups. The egg shell thickness of T2 showed a significant decrease compared to other groups. The third treatment showed
... Show MoreLighting is a very important element of treatment if the color contains many imaging system (digital cameras) and the unit of light and the light within these units are not strong , but usefel when the light is low , in different lighting intensities conditions image quality will not persist good enough and image may become dark or slightly exposed to light which leads to lower the details in image where we can not modify contrast or light ness to compensate thr decrease without losing the light and dark deatials . So we went in this research to study the variation colored texts written on the painting and lighting cases of non –regular ( a few) and different distances . As the diversity of these texts written on the board a
... Show MoreThis study presents a comprehensive set of laboratory works for the examined soil layers extracted from Baghdad city (specifically from Alkadhimya, Alaitaifiya, and Alhurriya) to illustrate their engineering properties. The researchers have adopted the unified soil classification system for soil classification purposes. Also, the direct shear test was performed for soil samples with various degrees of saturation (0%, 25%, 50%, 75%, and 100%). The test results have shown a significant reduction in cohesion property with higher moisture content within soil samples. Also, a noticeable reduction in angle of internal friction value has occurred with such changes. Furthermore, it has been found that the bearing capacity of unsaturated soi
... Show MoreA laboratory experiment was carried out and repeated at field of College of Agricultural Engineering Sciences, University of Baghdad in 2017. First factor was three cultivars of lupine 'Giza-1', 'Giza-2' and 'Hamburg'. Second factor was three seed weights (lower weight, medium weight and higher weight) which was following the cultivars factor. Nested design was used. Results showed supremacy of 'Giza-1' cultivar significantly and gave higher germination ratio, radical length, seedling dry weight, seedling vigour index, field emergence ratio, plant height and number of leaves per plant. The treatment ('Giza-1'×higher seed weight) was supremacy significantly and gave higher germination ratio, radical length, plumule length, and seedling vigo
... Show MoreWheat (Triticum aestivum L.) is one of the Poaceae family (Gramineae). Is momentous for human nutrition, and the stresses can affect strongly on the phenotype characteristics of the plant. The aim of this study was to determine how electric shock on germinated grain (for 2.5, 5, and 7.5 mins.) and heat shock at ( 35 oC, 40 oC and 45 oC) applied after phase out the radical length of 2-5 mm, from the grain of two wheat Varieties: “Baghdad 1” and “Babylon 113”. The electric shock for 2.5 mins., lead to delay of flowering, from day to 50 % flowering was 93 day, as well as, gave lowest value of plant height 64.5cm and lowest spike length was 10.7 cm. While The highest flag leaf area was obtained by electric shock for 5 mins. was 56.6
... Show MoreTo know the effect of bio-enhancer (zeolite), biohealth, mineral fertilizers and their interactions, the possibility of replacing mineral fertilizers with bio-enhancers and bio-enhancers, and their effect on some potato yield measurements. A field experiment was conducted at one of the field stations of the College of Agricultural Engineering Sciences, University of Baghdad, near the electronic calculator center, research station (F) in Al-Jadriya region in the loam mixture soil during autumn season 2021-2022 AD, It was designed using a completely randomized block design (RCBD) with three replicates. The factors of the study experiment included three levels of zeolite (0, 6 tons ha-1, and 12 tons ha-1), which were symbolized by (Z0)
... Show MoreThe field experiment was conducted at botanical garden of Department of Biology, College of Education for Pure Science/ Ibn-Al-Haitham, University of Baghdad to study the effect of Lepidium sativum leaves extract and pumpkin seeds Cucurbita pepo in two concentrations 10% and 20% on physiological characters ofPimpinella anisum. The results showed that in either concentration plant heights, dry weight, biological yield, economic yield, weight of 100 fruits, harvest index, absolute growth rate (AGR) were all increased and percentage of oil in fruits as compared with control plants
This study focused on the bactericidal potency of toluidine blue” TBO”photosensitizer and red laser radiation of 635nmwith different doses against multi-drug resistant streptococcus pyogenes (S. pyogenes) isolated from infected burns wounds to see if it is susceptible to photodynamic inactivation .
Atotal of 45 isolates were collected from 38” patients” with infected burnwounds samples were collected from September to December 2019.Burns wounds swabs were employed using standard procedures of swab collection. Among these, eleven isolates were multidrug resistant”S.pyogenes”. More resistant isolates that has been proved to all antibiotics used. This multidrug resistant isolate used in
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