This study included 50 blood samples collected from children with mean age 8-12 years. Thirty five blood samples were collected from children with Type 1 Diabetes Mellitus (T1D) with mean age 9.4±0.34 years, and 15 blood samples collected from healthy children as a control sample with mean age 10.9±0.38 years. Immunogenetic study was done on collected blood samples. Concentrations of IFN-γ were estimated from T1D patient and control samples by using Elisa instrument. The concentration of this interferon was 1.575 pg/ml in T1D patient sample in comparison with 0.921 pg/ml in control sample. Significant differences of this interferon concentration were found between T1D patient and control samples when Mann-Whitney U test was used

Two groups of chronic hepatitis B and C virus patients were divided into Pre-treated patients (25 CHB patients with positive HBs Ag for more than 6 months and 40 CHC patients), and post-treated patients [12 CHB patients (4, 6, and 2 were treated with lamivudine, IFN-? and combination of LMV + IFN-? respectively), and 27 patients for CHC (3, 13 and 11 patients were treated with Ribavirin, IFN-? and combination therapy (RBV+ IFN-?) respectively].These patients were followed up for 6 months. By using ELISA technique, levels of IL-6, IL-10, IFN-? and TNF-? were measured in vivo and in vitro (supernatant of PBMCs stimulated with PHA) and compared with healthy control. The mean level of IL-6, IL-10 and TNF-? in CHB patients showed significant dif

The research included an epidemiological study of cutaneous leishmaniasis in Baghdad for two years (2019 and 2020). Data were collected from the Iraqi Ministry of Health in Baghdad public health departments for Al-Karkh and Al-Rusafa districts. The results of the positive examinations for cutaneous leishmaniasis for the two years mentioned came with 54 infections in Al-Rusafa district and 80 infections in Al-Karkh district for the year 2019, while in 2020, the infections in Al-Rusafa district were 201 infections and 152 infections in Al-Karkh district. The data were divided in terms of gender into males and females, so the percentage for the year (2019) was 15% for males and 12% for females, and in (2020) 44% for males and 29% for female

Blood samples of One hundred and twenty patients from different hospitals in Baghdad infected with hydatidosis in different sites of the body (Liver, Lung, multiorgans and kidney) were collected for this study. On the other hand, 30 healthy individuals were included as a control group. This study was conducted to evaluate the effect of this disease on the serum protein profile of the patients using electrophoresis. The results revealed four different protein banding patterns with difference in number of bands and their molecular weights in comparison to the control group, and these differences depended on the site of infection. However the data showed a presence of the same band in all patients with different site of infection.

AbstractBackgroundLeishmaniasis is endemic in Iraq, where both cutaneous and visceral forms of the disease are reported.ObjectivesTo determine the prevalence of cutaneous leishmaniasis (CL) and to identify associations of CL with age, sex, season, and provinces depending on some demographic and climatic aspects.MethodsThis study is retrospective and includes reported cases of infections using the available surveillance database taken from the Iraqi Ministry of Health for the years 2011, 2012, and 2013 for all provinces of Iraq.ResultsMen and boys were found to be at higher risk for CL compared with women and girls. The majority of cases were recorded among those in age groups 5–14 and 15–45 years old. Most cases were recorded from lowla

Leukemia is the most common cancer in children which causes death despite the high survival rate. Therefore,  new methods are required to find a suitable therapy. A small RNA called microRNAs (miRNAs) is used as a biomarker for cancer diagnosis and early prognostic evaluation. Expression levels of three miRNAs from the 3' arm (miR-142-3p, miR-223-3p and miR-146-3p) were detected in serum samples from 30 acute leukemic children and from 30 healthy individuals by using qPCR. The miR-142-3p and miR-146-3p profiles were significantly downregulated (P=0.0010 and 0.0012, respectively), while miR-223 was found to be significantly upregulated (P= 0.0044) in the pateints. Serum level of C/EBP-β

Evaluation of Dot. ELISA test for Diagnosis Visceral Leishmaniasis in Infected Children

  For the first time in Iraq, this study was conducted to evaluate the usefulness of Dot.ELISA, for detecting anti - Leishmania donovani antibodies in serum samples from suspected patient  (children under 8 years ) with Visceral Leishmaniasis V.L..  Sera from 73 V.L. , 60 Healthy controls, and 57   patient with other parasitic diseases other than V.L. (Amoebiasis, Giardiasis , Toxoplasmosis, Schistosomiasis , Hydatidosis, Ascariasis , Lupus Erythromatosus , Viral Hepatitis, and Cutaneous Leishmaniasis) were examined. Anti Leishmania donovani antibodies detected  in 71 out of 73  suspected Visceral Leishmaniasis . Data of this study showed that infection in male group was more than female group. Result o

Back ground: Type 2 Diabetes mellitus (T2D) is a common complication of all liver diseases. However clinical and experimental data suggest a direct role of HCV in the perturbation of glucose metabolism. The aim of this study is to investigate the role of HCV infection as a risk factor to develop type 2 diabetes mellitus, and to study the immunopathogenicity of HCV in diabetes mellitus patients, through the assessment of IFN-γ, TNF- α and IL-10 serum levels.
Objectives: Is to investigate the role of HCV infection as a risk factor to develop type 2 diabetes mellitus, and to study the immunopathogenecity of HCV in diabetes mellitus patients, through the assessment of IFN-γ, TNF- α and IL-10 serum levels.

Visceral leishmaniasis (VL) is a parasitic infection caused by an intracellular growth of Leishmania spp. in macrophage cells. The autoimmune disorder is a condition takes place when the immune system produces antibodies which incorrectly attacked its own body tissues. VL has been involved as an effect or on the autoimmune aspect. This study was conducted to identify the auto antibodies profile in patients infected with VL. The presences of auto antibodies in 21 Iraqi children infected with VL were tested for laboratory autoimmune aspect. The highest percentage of seropositive in Leishmania patients was observed for anti-ds DNA, anti-Mi-2, anti-Ku and anti-PCNA antibodies (90.5%, 90.5%, 90.5% and 61.9%) respectively, while the lowest percen