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Bioremediation of Petroleum Polluted Soils using Consortium Bacteria
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      This study was carried out to isolate opportunistic hydrocarbons oil-degrading bacteria and develop a consortium or a mixture of bacteria with high biodegradation capabilities which can be used in biological treatment units of the contaminated water before release. The biological processes in general are environmentally friendly and cost effective, as they are easy to design and apply; as such they are more appropriate to the public.

    The location of the study was in Al-Dora refinery sludge holes area. The samples were collected for three seasons (winter, spring and summer) each consisted of three months.  The sludge samples were analyzed for various physical and chemical parameters. Temperature values of the sludge were at maximum in summer season, reaching 32ËšC, whereas they were at minimum in winter (24 ËšC). The values of sludge pH were at maximum in summer (9.70) and minimum in winter (9.20). Turbidity levels were 382 NTU in spring and 353 NUT in winter. Biological oxygen demand (BOD5) was at maximum in summer (760) and (690 mg/l) in winter. The maximum dissolved oxygen (DO) value of 5.20 mg/l was recorded in winter, while the minimum was 3.80 mg/l recorded in summer. The maximum electrical conductivity (EC) was 17130 μs/cm recorded in summer, while the minimum was 16150 μs/cm recorded in winter. The maximum total dissolved solids (TDS) values were 10335 mg/l recorded in summer, while the minimum (10015 mg/l) was recorded in winter. The maximum total petroleum hydrocarbon (TPH) value (431 mg/l) was recorded in summer, while the minimum (367 mg/l) was recorded in spring. Finally, the maximum salinity value (9.90%) was recorded in spring, while the minimum (9.30%) was recorded in winter. Also, hydrocarbon compounds in sludge samples were measured using Gas Chromatography - Mass Spectrometry (GC-MS), and the result showed that they were composed of 31 hydrocarbon compounds.In the present work, nineteen sludge degrading bacterial strains were isolated from the soil near Al-Dora refinery hole by primary and secondary screenings using a modified mineral salt medium supplemented with 1% (v/v) sludge as a carbon source. The most efficient two sludge degraded isolates identified by VITIK 2 compact were Kocuria rosea and Bacillus amyloliquefaciens. The tow isolates and there mixture showed best growth at 30°C for 12 days, as shown by the measurement of the optical density of the liquid culture and the final oil concentration by spectrophotometer.

     The bacterial isolates in liquid media with 2% (v/v) sludge showed best growth and the maximum biodegradation percentage after 12-day incubation period, as determined by gas chromatographic (GC). The degradation values were 68.9, 93.8 and 95.5% for Bacillus amyloliquefaciens, Kocuria rosea and the mixture of the tow isolates, respectively. In optimum conditions of pH 7, 40°C, 12 days incubation, the mixed bacterial consortium showed maximum sludge degradation.

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Publication Date
Mon Jan 29 2018
Journal Name
Iraqi Journal Of Science
Study Antibacterial Activity of Honey Against Some Common Species of Pathogenic Bacteria
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Honey is one of the most products has been used as an antimicrobial agent since the ancient times. The potential antimicrobial activity of honey is arise from its physiochemical proprieties such as high osmotic pressure, low pH, in addition to other factors and enzymes that work as antimicrobial agents. All these components and characteristics of honey led to consider it as one of the most efficient, useful and natural antimicrobial agents. From this point of view, recent study aim to evaluate the antibacterial activity of bee honey on some common species of pathogenic bacteria. The study included some of both gram-positive (Staphylococcus aureus, Staphylococcus epidermidis and Streptococcus spp.)

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Publication Date
Mon Jun 19 2023
Journal Name
Journal Of Engineering
Theoretical Study of the Effect of Permeability Tensor upon Drainage of Soils
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the influence of permeability tensor upon drainage of anisotropic soils under ponded water and steady recharge (rainfall) is theoretically investigated. Tensorial permeability has led to the formulation of mixed type partial differential equations. Since there is no analytical solution to this problem, the formulation is therefore solved numerically by the method of finite elements. The finite element formulation is implemented into a computer model which can be applied to any problem of seepage under steady state
conditions. Two different example problems representing two different flow conditions under full anisotropy have been studied. Results of the model for the isotropic case were checked against exact mathematical solutions de

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Publication Date
Tue Aug 15 2023
Journal Name
Sumer 1
Evaluation of the effects of Curcumin gel against Periodontopathic Bacteria (Porphyromonas gingivalis) using real-time time-polymerase chain reaction technology
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Periodontal disease is typically treated with mechanical debridement of the tooth surface. It may, however, be insufficient to eradicate pathogenic microorganisms on its own. Because of the microbial etiology of periodontitis, systemic or local antibiotic therapy is used as an adjunct treatment. The present study aimed to determine the effects of curcumin gel on Porphyromonas gingivalis. Eleven patients with stage II and III periodontitis were registered in the study. A double-blinded split-mouth design followed. Periodontal pockets were distributed into 2 groups; the test group received scaling and root planing along with curcumin gel, while the control group received scaling and root planing along with a placebo gel. Plaque index,

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Publication Date
Sun Sep 29 2019
Journal Name
Iraqi Journal Of Science
Radioactivity of Some Soils in the University of Tikrit, Iraq, college of Education for Girls using Solid-State Nuclear Track Detector type CR-39
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     In this research radon concentrations in soil samples of some sites of the College of Education for Women, University of Tikrit, were measured using CR-39 nuclear impact detector. Soil samples were  prepared according to classical protocols whereby they were irradiated for a period of 65 days in propagation chambers chemically treated and subjected to optical microscopy to calculate nuclear effects. The results show differences in the concentrations of radon gas in the samples collected from different sites  ranging between a lowest value in the location of the department of English and a highest value of  in the location of the cafeteria of College compared to the radiation background of due to th

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Publication Date
Thu Jun 01 2023
Journal Name
Online Journal Of Veterinary Research
Article review: Infections by gram + bacteria in laboratory mice
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Publication Date
Sun Sep 05 2010
Journal Name
Baghdad Science Journal
Effect of some species of bacteria on viability of human hydatid cysts
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A total of 50 fertile human hydatid cases {33(66%) females and (34%) males}, obtained from Al-Ramadi public Hospital during the period from December 2003 to July 2004 were examined to study any bacterial infections. The specimens were obtained from hydatid fluid and then cultured on appropriate culture media to distinguish some species of bacteria which resulted in obtaining: Staphylococcus aureus (18%), Pseudomonas aeruginosa(12%), Escherichia coli(6%) and Streptococcus pneumonia (4%). These bacteria were confirmed by isolation from interacyst fluid and blood culture technique. The possible routs of infection may be through blood, biliary ducts and bronchioles .The selectivity permeable of the cyst wall may be absent and that may allow

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Publication Date
Sun Mar 31 2013
Journal Name
European Scientific Journal, Esj
EFFECT OF SALT CONTENT ON TOTAL AND MATRIC SUCTION OF UNSATURATED SOILS
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Soils located above the groundwater table are generally unsaturated and possess negative pore-water pressures. A soil-water characteristic curve (SWCC) that relates the water content of a soil to matric suction is an important relationship for the unsaturated soil mechanics. The SWCC essentially shows the ability of an unsaturated soil to retain water under various matric suctions. The filter paper method is a soil suction measurement technique. Soil suction is one of the most important parameters describing the moisture condition of unsaturated soils. The measurement of soil suction is crucial for applying the theories of the engineering behavior of unsaturated soils.In this paper, three soil samples were collected from three sites within

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Publication Date
Sun Jan 01 2023
Journal Name
Journal Of Population Therapeutics And Clinical Pharmacology
Purification and Characterization of Antifungal Protein from Bacteria
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Publication Date
Tue Sep 01 2020
Journal Name
Gene Reports
Efflux pumps of Gram-negative bacteria in brief
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he development of multidrug resistance in Gram-negative bacteria is a major problem faced antimicrobial therapy and management of infectious diseases. Too many resistance mechanisms were developed since the antimicrobial agents were produced and actually used. The mechanisms involved in antimicrobial agents' resistance are, modifying enzymes, alteration of the target site of antimicrobials and prevention of antimicrobials accumulation inside the bacterial cells. The latest is accomplished by two mechanisms: alteration of outer membrane permeability and efflux pumps. Efflux pumps are either chromosomal or plasmid-encoded although chromosomal encoded efflux pumps are common in Gram-negative bacteria. Resistance nodulation division (RND) efflu

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Publication Date
Wed Feb 22 2023
Journal Name
Iraqi Journal Of Science
Health Risk Assessment of Polycyclic Aromatic Hydrocarbons in Surface Soils at North Baiji City, Iraq.
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Four major factories (Petroleum Refineries Company, Detergents Plant, Thermal Power Plant, and Gaseous Power Plant) are located to the north of Baiji City. They release pollutants in form of gases, liquids and solids; they find their way to the surrounding environment. To assess the environmental pollution of the area, 18 samples of surface soil distributed around the industrial establishments were collected and analyzed to determine the concentration of polycyclic aromatic hydrocarbons (PAH) components which are often targets in the environmental checking. Identification and quantification of the 16 PAHs components was accomplished using High Performance Liquid Chromatography (HPLC) had a model Shimadzu LC-10 AVP. The total concentratio

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