Simple and sensitive batch and flow injection methods (normal and reverse flow injection analysis (nFIA and rFIA)) for spectrophotometric determination of vancomycin hydrochloride (VHC) in pharmaceutical preparations were proposed and optimized. Both methods are based on the oxidative - coupling reaction between vancomycin hydrochloride and 2,4-Dinitrophenylhydrazine (DNPH) in the presence of sodium periodate in alkaline medium to form a yellow water-soluble product that is stable and has a maximum absorption at 461 nm. Beer’s law was obeyed over the range of 1- 40, 0.5-120 and 0.5-150 μg.mL-1; the limits of detection were 0.537, 0.0823 and 0.233 μg.mL-1 for batch, normal and reverse flow injection methods respectively. The sampling rates were 124 and 120 injections per hour for normal and reverse flow injection methods respectively. The effects of chemical and physical parameters have been carefully considered and the proposed procedures were successfully applied to the determination of vancomycin hydrochloride in pharmaceutical preparations
The mutagenic effect of some crucifers widely consumed Lepiduim sativum (Garden cress) and Arugula (Eruca sativa) was studied in comparison to carrot (Daucus carota), using bacterial mutagenic system composed of three bacterial isolates; (Bacillus spp)G3 (Arthrobacter spp)G12, and (Brevibacterium spp)G27 ,. Treatment of isolates with plant extracts led to reduction in survival fraction (Sx)at different levels except that Aurgula extract did not show any inhibitory effect in isolates G12 and G27. Crucifers extracts induced streptomycin resistant mutants in G12 at higher level than G3 , but not in G27. No resistance to rifimpacin was detected in all isolates.
Background: The diagnosis of prostatic pathology may be of challenging , as some difficult and suspected, atypical cases may lack basal cell layer by routine H&E sections . Antibodies against 34BE12(HMW-CK) and p63 aid the diagnosis of such cases , to distinguish benign from malignant prostatic lesions.
Objective: to identify basal cells in atypical prostatic lesions ,and distinguish benign from malignant prostatic lesions.
Type of the study: A retro-spective study.
Methods: 115cases of paraffin embedded prostatic tissue blocks ,diagnosed as : 76 cases were benign prostatic hy
... Show MoreDensities
ï² and viscosity
ï¨ of serine in 20, 40, and 60% (w/w) dimethyl sulfoxide (DMSO)-water mixtures were measured at 298.15, 303.15 and 308.15k. From these experimental data, apparent molal volume v ï¦ , limiting apparent molal volume v ï¦ o , the slop v S , transfer volume v ï¦ o(tr), Jones-Dole coefficients A and B were calculated. The results are
v ï¦ odiscussed the solute-solvent and solute-solute interaction, and showed that serine behaves as structure-breaker in aqueous DMSO solvent
The present study was conducted to investigate the resistance of fluoroquinolones (FQs) and the effects of mutations in the resistance gene in clinical isolates of P. aeruginosa isolated from different sources in Al-Hussein Hospital, Al-Samawah city, Iraq. The basic mechanism of the resistant of fluoroquinolones in P. aeruginosa is via mutations occurring in the basic bacterial gyrA gene encoding-subunit A of DNA gyrase . Forty clinical isolates from various sourced (burn 7 (17.5 %), wound 7 (17.5 %), ear 2 (5 %), operation room 12 (30 %), urine 3 (7.5 %), and industrial dialysis center 9 (22.5 %)) were isolated based on bacteriological methods confirmed by 16s rRNA gene using PCR technique. A se
... Show MoreIn this paper, an experimental analysis of conventional air-cooled and microchannel condensers in automotive vapor compression refrigeration cycle concerning heat transfer coefficient and energy using R134a as a refrigerant was presented. The performance of two condensers and cycles tested regarding ambient temperature which it was varied from 40oC to 65oC, while the indoor temperature and load have been set to be 23oC and 2200 W respectively. Results showed that the microchannel condenser has 224 % and 77 % higher refrigerant side and air side heat transfer coefficient respectively than the coefficients of the conventional condenser. Thus, the COP, in case of using the microchannel
... Show MoreThe structural division and stratigraphic estimation of the perceptible geological basin are the most important for oil and gas exploration. This study attempts to obtain subsurface geology in parts of east Nasiriya, southern Iraq using of seismic data and some adjacent well information for structural and stratigraphic interpretation. To achieve this goal, 2D seismic data in SEG-Y format were used with velocity and logging data. The seismic profile is then interpreted as a two-dimensional (time domain and depth domain) contour map, which is represented as a real subsurface geology.
Reflectors from the Mishrif and Yamama Formations (Cretaceous period) were detected. According to the structural interpretation of the sel
... Show MoreObjective(s): assessment of the health follow up and weight control for women with osteoporosis and find out the relationship between their health follow up and weight control and their socio-demographic characteristics.
Methodology: A descriptive study was conducted on women with osteoporosis for the period of September, 26th 2020 to Jun, 20th 2021. Non- probability (convenient) sample of (70) women with osteoporosis selected from (5) Private Clinics for Joints and Fractures in Baqubah City. A questionnaire was designed though extensive review of literatures and it consists of three parts: the first part includes women’s socio demographic characteristics, the second part inclu
... Show MoreThe present study aimed to evaluate the effect of lead exposure on hemopoetic system (through the index delta-Aminolevulinic acid dehydratase ?-ALAD activity & hemoglobin concentration (Hb) ) and on iron status (levels of iron Fe, Ferritin Fr, Total iron binding capacity TIBC, percentage of transferine saturation TF%) in 44 Iraqi worker at lead batteries factory. Workers divided into two groups: smokers(n=21) mean aged (37.33±4.82 year)and non smokers(n=23) mean aged(40.78±7.89 year) and 45 healthy subjects mean aged (33.97±5.08)as control group . Activity of ?-ALAD ratio shows significant decrease (p ? 0.05) ,while Hb and hematocrit Hct were non significant (p ? 0.05) in smoker workers more than non smoker as compared to control . The r
... Show MoreEngineering equipment is essential part in the construction project and usually manufactured with long lead times, large costs and special engineering requirements. Construction manager targets that equipment to be delivered in the site need date with the right quantity, appropriate cost and required quality, and this entails an efficient supplier can satisfy these targets. Selection of engineering equipment supplier is a crucial managerial process .it requires evaluation of multiple suppliers according to multiple criteria. This process is usually performed manually and based on just limited evaluation criteria, so better alternatives may be neglected. Three stages of survey comprised number of public a
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