A newly photometric analytical method characterized by its speed and sensitivity was developed for the determination of folic acid in pure and pharmaceutical samples via its oxidation to reddish-orange coloured complex through oxidation by cerium (IV) sulphate in aqua medium using homemade Ayah 3Sx3-3D-solar cell CFI photometer. The colored species were determined using supper bright green light emitting diode (LED) as a source. A 100μl was taken as a best sample volume for the determination of folic acid. The linearity of calibration curve for the instrument response versus folic acid concentration was 0.005-20 mmol.L-1 while the L.O.D. was 0.5 mol.L-1 from the stepwise dilution for the minimum concentration of lowest concentration in the linear dynamic range of the calibration graph. The correlation coefficient (r) was 0.9994 while the percentage linearity (r2%) was 99.88%. The method was applied successfully for the determination of folic acid in pharmaceutical preparation. RSD% for 8 mmo.L-1 (n=6) less than 1% . Using paired t-test it was shown that there was no significant difference between the proposed method and official method (Quoted value- 5mg) and on that basis the new method can be accepted as an alternative analytical method.
New Approach for the On-Line Spectrophotometric Determination of Folic Acid in Pure and Pharmaceutical Preparation via Oxidation by Cerium(IV) Sulphate Using Ayah 3Sx3-3D-Solar Cell CFI Spectrophotometer Analyzer
Flow injection analysis
Folic acid
Spectrophotometer
Colorimetry
Publication Date
Sun Oct 01 2006
Journal Name
Journal Of The Faculty Of Medicine Baghdad
Alcoholic Liver Disease: Alfa Fetoprotein Alteration, Hematological & Biochemical Characteristics
Alcoholic liver disease
Alfa fetoprotein
gamma glutamyl transpeptidase. Statistical analysis: Chi square tests and the mean value with SD for each value were determined using ANOVA. A p value of <0.05 was considered to be positive.
Background: Alcohol remains the single most significant cause of liver disease throughout the Western world, responsible for between 40 and 80% of cases of cirrhosis in different countries. Many of the factors underlying the development of alcoholic liver injury remain unknown, and significant questions remain about the value of even very basic therapeutic strategies.
Patients and Methods: In a cross sectional study, 113 alcoholic patients with evidence of liver disease in the absence of other significant etiology attending the Gastoenterorology and Hepatology Teaching Hospital between December 2001 and December 2003 were studied for the hematological and biochemical spectrum of alcoholic liver disease in