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Optimum conditions of keratinase production from Bacillus licheniformis
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Forty isolates of Bacillus spp. were isolated from fifty samples including different source of soil to detect the ability to produce keratinase enzyme in liquid state fermentation, Bacillus (Bs13)was the highest keratinase producer , it was identified as a strain of Bacillus licheniformis. The optimum conditions for keratinase productions were in a media contains keratin 4% (hooves) as a carbon and nitrogen and energy sources, peptone 1% as a secondary nitrogen source with pH 8 , inculums size 1%, and incubated at 37Co for 24 hrs.

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Publication Date
Tue Mar 14 2023
Journal Name
Iraqi Journal Of Science
The Optimum Conditions for Pleurotin Production by Pleurotus spp. Local Isolates
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The study includ selection of six species of the fungi related to genus Pleurotus were evaluated for their ability to produce of Pleurotin, one of them, Pleurotus ostreatus (P.11) was isolated and identified in the present study. Pleurotin was detected by Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC). The maximum absorption of Pleurotin was 1.6 nm at 250 nm. Pleurotin was purified with two methods using chloroform and ethyl acetate, the results showed the ethyl acetate was more efficient in pleurotin production resulting in 14.6 μg/ml compared to 9.8 μg/ml with chloroform. The local isolate, P. osteratus (P.11) showed significant high Pleurotin production (14.6 μg/ml) when was grown on the modified

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Publication Date
Thu Feb 10 2022
Journal Name
Iraqi Journal Of Science
Determination of optimum conditions for xylanase production by Aspergillus niger using solid state fermentation
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Aspergillus niger is one of the most important filamentous fungi that used in the fermentation industry. Aspergillus niger isolate was cultured on potato-dextrose agar (PDA) for activation, and the optimum conditions for xylanase production from this local isolate were studied by solid state fermentation, using a medium composed of wheat bran moisten with corn steep liquor at ratio 1:0.5 (v:w) at initial pH 5.5, inoc-ulated with 1.6 × 106 spores/ml, and incubated at 30ᵒC for 5 days.

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Publication Date
Sun Jul 02 2023
Journal Name
Iraqi Journal Of Science
Determination of Optimal Conditions for the Production of Laccase Enzyme by Local Isolate of Bacillus sp.
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Fifty isolates of Bacillus sp. were subjected to the first and second screening to detect the ability to produce laccase enzyme and select the highest ones production of laccase on Petri plates containing nutrient agar supplemented with Cu2+.
Syringaldazine was used as an indicator and substrate for the determination of laccase activity. Three isolates, which consumed less time to developed pink color were tested for the production of laccase quantitatively. The effective isolate B16 with significant amounts of laccase 1.84 unit /ml was selected for laccase study.
The optimization studies revealed that the maximum laccase production was achieved when the production medium was at the following conditions: 5 days of incubation, tempe

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Publication Date
Sun Jun 07 2015
Journal Name
Baghdad Science Journal
Optimum conditions for Inulinase production by Aspergillus niger using solid state fermentation
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Thirty local fungal isolates according to Aspergillus niger were screened for Inulinase production on synthetic solid medium depending on inulin hydrolysis appear as clear zone around fungal colony. Semi-quantitative screening was performed to select the most efficient isolate for inulinase production. the most efficient isolate was AN20. The optimum condition for enzyme production from A. niger isolate was determined by busing a medium composed of sugar cane moisten with corn steep liquor 5;5 (v/w) at initial pH 5.0 for 96 hours at 30 0C . Enzyme productivity was tested for each of the yeast Kluyveromyces marxianus, the fungus A. niger AN20 and for a mixed culture of A. niger and K. marxianus. The productivity of A. niger gave the highest

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Publication Date
Sun Mar 01 2009
Journal Name
Al-khwarizmi Engineering Journal
The Optimum Conditions for Production of Soya Peptone by Acidic Hydrolysis of Soya Proteins
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     This study was carried out to obtain the optimum conditions necessary for the process of soya protein hydrolysis by using hydrochloric acid (as a chemical catalyst) instead of the papain enzyme (as a biological catalyst), for the production of soya peptone. These conditions are implemented to test the effect of the variables of the process of hydrolysis on the nature and quality of the product.

        The production of soya peptone was studied for their importance in the process of preparing and producing the culture media used in medical and microbiological laboratories.

      The process of production of soya peptone includes four main

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Publication Date
Wed Sep 01 2021
Journal Name
Journal Of Ecological Engineering
Study of The Fermentation Conditions of the Bacillus Cereus Strain ARY73 to Produce Polyhydroxyalkanoate from Glucose
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Polyhydroxyalkanoates (PHAs) have gained much attention as biodegradable polymers, many efforts are being made to minimize the cost of PHAs by finding cheap carbon source depending on the type of microorganism and fermentation conditions. The aims of this study were to evaluate the effects of different glucose concentrations and other important conditions on the PHA production by Bacillus cereus isolated from soil. Polyhydroxyalkanoates PHAs accumulated by soil microorganisms were examined by screening the isolated bacteria using Sudan B Black and Nile Blue staining process. A Gram positive strain was identified using the 16s rRNA gene, deposited in the NCBI GenBank sequence database. Different growth conditions (favorite glucose concentrat

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Publication Date
Wed Mar 10 2021
Journal Name
Baghdad Science Journal
Study of some effective factors on the production of garamicidin from locally isolated Bacillus brevis
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The optimum cultural conditions for garamicidin production by local isolate B.brevis were studied.Best result was obtained when the isolate B.brevis was grown on media composed of 1%glucose as carbon source,1% ammonium chloride as a nitrogen source ,0.5% Dipotassium hydrogen orthophosphate as a phosphate source and after 48 hours of incubation at 30C .Garamicidin has been extracted and purified through acid precipition and then extracted by organic solvent (ether& acetone ).Using HPLC the garamicidin antibiotic showed three types A,B and C garamicidin .

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Publication Date
Mon Oct 30 2023
Journal Name
Iraqi Journal Of Science
Optimize and Purification of Keratinase Produced from Local Aspergillus Terreus A13 Isolate Using A Feather as Substrate
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     Keratin is a fibrous, insoluble structural protein that is highly cross-linked with hydrophobic, hydrogen, and disulfide bonds. Keratinases are enzymes that belong to the category of serine hydrolases that are capable of breaking down keratin. The results of the determination of the better fermentation system showed that the production of keratinase from local A.terreus A13 isolate by submerged fermentation (SmF) system was the best system to give the highest specific activity (113.4 U/mg) of keratinase compared with solid-state fermentation (SSF). The optimum conditions for keratinase production by SmF, were determined via cultivation conditions, including carbon source, nitrogen source, temperature, pH of the medium,

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Publication Date
Sun Jul 02 2023
Journal Name
Iraqi Journal Of Science
The Optimum Conditions for Naphthalene Biodegradation by Filamentous Fungi
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Recent studies have proved the important role of fungi in the biodegradation of oil pollutants. The present study aims to find the optimal conditions for the fungi to get the best rate of the biodegradation of the polycyclic aromatic hydrocarbon (PAHs) (Naphthalene) compounds. Soil samples were taken from 18 different sites polluted with oil wastes and cultured then obtained 312 isolated fungi from 64 replicates Primarily screening were done on fungal isolates on solid media containing naphthalene the results revealed that 25 fungal isolates gave good growth, 47 fungal isolates gave Moderate growth, 66 gave weak growth and 147 fungal isolates gave no growth on Naphthalene solid media.
Then secondary screening were done on 25 fungal is

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Publication Date
Sun Oct 22 2023
Journal Name
Iraqi Journal Of Science
The Optimum Conditions for Naphthalene Biodegradation by Filamentous Fungi
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Recent studies have proved the important role of fungi in the biodegradation of oil pollutants. The present study aims to find the optimal conditions for the fungi to get the best rate of the biodegradation of the polycyclic aromatic hydrocarbon (PAHs) (Naphthalene) compounds. Soil samples were taken from 18 different sites polluted with oil wastes and cultured then obtained 312 isolated fungi from 64 replicates Primarily screening were done on fungal isolates on solid media containing naphthalene the results revealed that 25 fungal isolates gave good growth, 47 fungal isolates gave Moderate growth, 66 gave weak growth and 147 fungal isolates gave no growth on Naphthalene solid media.
Then secondary screening were done on 25 fungal is

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