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Possible Protective Anticancer effect of Ethanol Fraction of Iraqi Hibiscus Tiliaceus L. Leaves Extract on Diethylnitrosamine-induced Hepatocarcinogenesis in Male Rats
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Abstract  Liver cancer with hepatocellular carcinoma a serious clinical illness that progresses quickly and has a bad prognosis because to increased malignancy. Fibrosis is the precursor of liver cancer, which progresses to cirrhosis and carcinoma Diethylnitrosamine (DEN) is a chemical molecule that has been used as a carcinogenic agent to promote cancer in test animals because of its strong carcinogenic potential. Herbal plants have long been used as inexpensive, effective alternatives to pharmaceuticals in various liver-associated complications, since they contain many bioactive compounds useful in liver disorders. Hibiscus tiliaceus L. (Malvaceae) contain various phytochemicals in the plant extracts such as Flavonoids, phenols, alkaloids, Tannins, Saponins, Quinones, Coumarine, Triterpenoids, glycosides and Steroids. Thus, has various health benefits, and have medicinal and therapeutic values, It could be a source of natural antioxidants, antimicrobials, hepatoprotective compounds, and antimutagenic effects. The study was designed To study the possible protective role of Iraqi Hibiscus tiliaceus L. ethanol leaves extract administered in two different doses on diethylnitrosamine induced liver carcinoma in male Wistar Albino rats. Rats utilized in this study were randomized into 4 groups (six rats per each group); Group I: (Control) Rats were administered oral daily dose of 1ml /kg/day of distilled water for 20 weeks; Group II: rats intraperitoneally injected with 70 mg/kg diethyl nitrosamine once per week for 10 continuous weeks; Group III: Rats were administered 250mg/kg of Iraqi Hibiscus tiliaceus L.  ethanol leaves extract as chronic oral administration with food for 5 days per week for 20 weeks and subsequently intraperitoneal dose of diethylnitrosamine (70 mg/kg) once per week for 10 continuous weeks; Groups IV: Rats were administered 500mg/kg of Hibiscus tiliaceus L. ethanol leaves extract as chronic oral administration with food for 5 days per week for 20 weeks with subsequently intraperitoneal dose of diethylnitrosamine (70 mg/kg) once per week for 10 continuous weeks. The finding revealed that Iraqi Hibiscus tiliaceus L.  ethanol leaves extract showed significant reduction (P<0.05) in malondialdehyde, alpha fetoprotein (AFP), alanine aminotransferase (ALT), and aspartate aminotransferase (AST), total bilirubin (TSB), pro-inflammatory cytokines include transforming growth factor beta (TGFβ), tumor necrosis factor alpha (TNFα) and significant elevation (P<0.05) of anti-oxidant enzyme superoxide dismutase (SOD) in dose dependent manner. In conclusion this study demonstrated that the chemoprotective role of Hibiscus tiliaceus L. ethanol leaves extract might be explained by; lowered levels of hepatic enzymes, pro-inflammatory cytokines, and antioxidant levels, as well as decreased levels of alpha fetoprotein (AFP), the gold standard for detecting hepatocellular carcinoma.

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Publication Date
Wed Jul 06 2011
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
The Effect of essential oil extracted from yellow peel of Citrus aurantium L. on growth of some fungi
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جريت دراسة مختبرية لمعرفة تأثير الزيت الطيار لقشور ثمار نبات النارنج الصفرC. aurantium تجاه النمو السطحي للفطريات Penicillium expansum، Aspergillus flavus و Fusarium oxysporum ، أظهرت نتائج الفعالية التثبيطية للزيت الطيار تأثيراً معنويا متفاوتاً في الفطريات المشمولة بالدراسة، إذ كان الزيت الطيار أكثر تأثيرأَ في الفطر P. expansum تلاه الفطر A. flavus ،في حين كان الفطر oxysporum F.أقل حساسية تجاه الزيت الطيار. بصورة عامة اظهر الزيت الطيار تأثيرا تثبيطيا

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Publication Date
Mon Dec 26 2022
Journal Name
Journal Of The College Of Basic Education
دراسة تشريحية مقارنة للأجزاء الخضرية لنوعين من جنس Hibiscus المستزرعة في العراق
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تضمنت الدراسة الحالية تفاصيل الخواص التشريحية للأجزاء الخضرية (الجذر، الساق، السويق، الورقة) لنوعين من جنس Hibiscus المستزرعة في العراق متمثلة في النوعين الباميا Hibiscus esculentus وورد الجمال Hibiscus rosa-sinensis إذ تناولت الدراسة الدليل الثغري ومعدل كل من طول وعرض المعقد الثغري وسمك البشرة المحيطة والقشرة واللحاء ونصف قطر الخشب وعدد أذرع الخشب ووحدات الخشب في الصف الواحد. وقد أظهرت الدراسة أن هناك تبايناً واضحاً في الخو

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Publication Date
Fri Dec 29 2023
Journal Name
International Journal Of Nanoscience
Detection of Silica in Rice Husks Using Laser-Induced Plasma and Studying the Effect of Laser Energy on the Parameters of the Produced Plasma
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This study aims to analyze the spectral properties of plasma produced from rice husk(Rh) using the laser breakdown spectroscopy (LIBS) method. The plasma generation process used the fundamental harmonic (1064 nm) of a Q-switched Nd:YAG laser. Yttrium aluminum garnet (YAG) is a man-made crystalline material. The laser fired pulses with a duration of 10 ns and a repetition rate of 6 Hz. Thus, the energy outputs achieved were 50–200 mJ at the wavelength of 1064 (nm). The silica content in the rice hulls was verified using an XRF measurement, which revealed the presence of silica in the rice hulls in a high percentage. Precise beam focusing was achieved by focusing the laser on the target material. This target material is placed with

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Publication Date
Wed Jan 01 2020
Journal Name
Annals Of Tropical Medicine And Public Health
Determination of hepcidin (25) level in hemodialysis Iraqi male patients and its relation with erythropoietin and some biochemical parameters
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Background: Anemia is a common finding in patients with renal failure disease and it is, primarily, due to reduced production of iron and erythropoietin. Therefore, the aim of current study was to evaluate the relationship between serum level of hepcidin 25, on the one hand, and levels of serum iron, total iron-binding capacity, serum erythropoietin and transferrin saturation% in Iraqi male patients undrgoning haemodialysis.. Methods: Serum from75 males in the end stage of hemodialysis, as group one, and 25 Iraqi healthy malecontrols, as group two, were recruited in this study. Results: Data from current study showed significant increase in hepcidin 25 and significant decrease in serum iron, total iron-binding capacity, transferrin saturati

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Publication Date
Wed Jan 01 2020
Journal Name
Systematic Reviews In Pharmacy
Impacts of the alcoholic extract and essential oil of Thymus vulgaris L. Against the causative agent of acne formation (Staphylococcus aureus)
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Medicinal plant life have performed an integral role in the development of human lifestyle being used as resources for the manufacturing of contemporary medicines, antibiotics. Microorganisms are responsible for many issues in industry and remedy because of biofilm formation. This study aimed to take a look at the results of Thymus vulgaris alcoholic extract on Staphylococcus aureus the causative marketers of acne in human particularly the women. The extract was acquired mixing 25 g of Thyme powder with 350 ml of 80% ethanol for 6 hours at 40°. Antimicrobial activities of the plant extract and integral oil towards the S. aureus were decided using the agar diffusion technique. The consequences proven that special concentrations of alcoholic

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Publication Date
Sun Mar 13 2011
Journal Name
Baghdad Science Journal
The Effect of Silybum marianum L. aquatic crude extracts on the cancer cell lines and normal cell line in vitro
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The aquatic crude extract of Silybum marianum dry grains prepared by melting them in distil water by the method of soak and shake. The effect of Silybum marianum crude extract studied in vitro on three tumor cell line the Hep-2, AMN-3 and RD for 24, 48 and 72 hours of exposure, and one cell line of normal cells REF for 72 hr exposure. The results showed that the prescence of toxic effect of the aquatic crude extract on the cell lines of Hep-2, AMN-3 and RD at 10 and 100 µg/ ml upto the higher concentrations when they exposed to the extract for 48 hr. as compared with the control treatment, and when the exposure period increased to 72 hr. the toxic effect started at low concentrations (5 and 10 µg/ ml) as compared with the control g

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Publication Date
Fri Sep 01 2023
Journal Name
Journal Of Education And Scientific Studies
Studying the effect of laser stress in combination with mannitol on some morphological parameters of cabbage (Brassica oleracea var L. capitata) In vitro
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Publication Date
Thu Feb 01 2024
Journal Name
Baghdad Science Journal
Studying the Effect of Curcumin (Standard & Supplements) and Zinc on the Concentrations of Glucose, Insulin, HOMA-IR, and Anti-Mullerian Hormone in PCOS-Model Rats
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The goal of the current study was to investigate the effects of curcumin in both formulas (supplement and standard), zinc, and then use them together to show their effect on the levels of glucose, insulin, insulin resistance (IR), and anti-mullerian hormone (AMH) in the model of female rats with induced polycystic ovary syndrome (PCOS) using 1mg/kg/day of letrozole for 21 days followed by a treatment period of 14 days including different treatments of zinc 30 mg/kg, curcumin standard 200 mg/kg, curcumin supplement 200 mg/kg, (curcumin standard plus zinc), (curcumin Supplement plus zinc) and metformin as a standard treatment. After the treatment, all female rats were sacrificed, and blood samples were collected from the inferior vena cava

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Publication Date
Thu Jul 18 2019
Journal Name
Annals Of Tropical Medicine & Public Health
SURVIVIN TARGETING; AN IN SILICO APPROACH FOR ANTICANCER DESIGN
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Survivin, a member of inhibitor of apoptosis family is increasingly used as a target for cancer therapy design because it has a key role in cell growth and inhibition of cell apoptosis. Also it can be used as a biomarker for targeting cancer because it is found in almost all cancer but not normal cells. Our strategy was to design (computationally) a molecule to be used as survivin inhibitor. This molecule was named lead10 and was used furthermore to find (virtually) existing drugs with a good survivin inhibition activity.

Publication Date
Mon Jan 01 2024
Journal Name
Baghdad Science Journal
Effect of nanocapsules and extract of Metarhizium anisopliae in inhibiting acetylcholine esterase enzyme in Musca domestica larvae.
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M. domestica is the most important insect that transmit pathogens for diseases in the world. The use of nanotechnology is eco-friendly method in control pests. The study aims to investigate the feasibility of bio-manufacturing nanocapsules of fungal secondary metabolites in order to improve the efficiency of metabolite and assess their inhibitory effect on the acetylcholine esterase enzyme in housefly larvae. An equal mixture of organic solvents, ethyl acetate and dichloromethane, was used to extract the metabolic products of the fungus M. anisopliae, (PEG4000) and chitosan was used in the preparation of nanocapsules. The results of the DLS granular size assay showed that the size of the extract particles and the size of the chitosan and

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