M. domestica is the most important insect that transmit pathogens for diseases in the world. The use of nanotechnology is eco-friendly method in control pests. The study aims to investigate the feasibility of bio-manufacturing nanocapsules of fungal secondary metabolites in order to improve the efficiency of metabolite and assess their inhibitory effect on the acetylcholine esterase enzyme in housefly larvae. An equal mixture of organic solvents, ethyl acetate and dichloromethane, was used to extract the metabolic products of the fungus M. anisopliae, (PEG4000) and chitosan was used in the preparation of nanocapsules. The results of the DLS granular size assay showed that the size of the extract particles and the size of the chitosan and (PEG 4000) nanocapsules were 610, 217 and 188 nm, respectively. The SEM images showed that the diameter of the extract and the nanocapsules chitosan and polyethylene glycol 4000 reached a rate 547.5, 17.8 and 26.2 nm, respectively. The FTIR showed that the extract of the second products of the fungus contains functional groups like: alkynes and alkenes, amines, carboxyl and aromatic groups, while the presence of groups of phenols, alcohol, amines, alkenes, and alkyl halides was recorded for nanocapsules of chitosan and PEG. The results showed that the extract of fungal metabolic and nanocapsules has an inhibitory effect on acetylcholinesterase enzyme and reached the highest inhibition rate 53.2 ,36.3,18.2% when treated with nanocapsules PEG at a concentration 500 ppm, extract of fungal metabolites at a concentration 50,000 ppm, chitosan nanocapsules at a concentration 500 ppm respectively. It is clear that acetylcholinesterase inhibition is one of the mechanisms of fungi metabolic action and the nanocapsules prepared from them.
This study consist of using Metarhizium anisopliae as abioinsecticide against house fly
Musca domestica L. For controlling it biologicaly its Larvae were treated by sprying them
directly and sprying their food with 2.3 × 10
8
conidia / ml of Metarhizium anisopliae .
Infected Larve showed symptoms of slugishness and slow growth. Histopathological study of
the dead Larve showed Conidial adhesion and germ tube penetrations through body wall and
begining of fungus hyphal growth under the body wall and hyphal invasion in haemocoel and
gut.
A study was conductedto determine thethe effects of two insect pathogenic fungiMetarhizium anisopliae and Beauvaria bassiana In biological control and comparing, insecticide, Icon, on larva , pupa and adults stage of house fly was tested. The results revealed non-significantly superiority differences in the percentages of cumulative death between the recommended concentration of Icon and the M.anisopliae(2 × 1011), but the results showed, significant difference in accumulation death between M. anisoplia ( 2 × 1011 ) and B. bassiana were found at the same concentration, the accumulation death of M.anisoplia reached to 100, 80, 95% for larval, pupa and stage after 96 hours respectively, com
... Show MoreThe house flies Musca domestica )Diptera:musidae) are the primary carrier of many pathogens such as cholera, typhoid, anthrax, and others. The use of chemical pesticides as a basic method of control leads to many problems at the environmental and health level. The use of safe alternatives to chemical pesticides has become an urgent necessity. The research aims to find biological alternatives that are environment-friendly and non-pathogenic to humans in controlling house flies through the possibility of extracting and diagnosing some secondary metabolites produced by the fungus Metarhizium anisopliae and testing their effects on the second larval stage of house flies using different treatment methods that include
... Show MoreThe main aim of this study is to investigate the ability of four local entomopathogenic isolates Beauveria bassiana (Bals.) and Metarhizium anisopliae (Met.) to control the mosquito larvae in the lab. The results revealed that the isolate (MARD48) B .bassiana reduced the survival rate of the mosquito larvae to (80%) followed by the isolate M. anisopliae (MARD10) to (90%) in the first two days of treatment, and 60 and 66% respectively in the third day. The results also showed that the isolate B. bassiana (MARD48) killed 50% of the population (LC50) with the concentration 1×104 conidia/ml compared to 1×107 conidia/ml for the isolates B. bassiana (MARD14) and M. anisopliae (MARD10), and 1×108 conidia/ml for the isolate B .bassiana (MARD76).
... Show MorePresent study is carried out to evaluate the effect of alkaloids extract of
Amaranthus gracilis on some biological aspects of the house fly Musca domestica to
find out the best way for their control. The highest mortality percentage on eggs of
the house fly was 83.3% at concentration of 10 mg/ml followed by 44.4% and
31.1% at 7.5 mg/ml and 5 mg/ml respectively. Results also referred that the
mortality rates of eggs, larvae, pupae were increased with increasing concentration.
The pupal weights were decreased with increasing of concentration.
This was study conducted to evaluate the efficacy of the secondary metabolites from produced Metarhizium anisopliae in potato sucrose broth, rice broth and wheat broth, against larvae and adults of Chysomya albiceps. Results showed that filtrate of potato broth was better in mortality rates occurrence when second instar larvae were treated, the mortality rate was 46.67%. While the treatment of the larvae using the filter of rice and wheat broth gave total accumulated mortality rate (larvae and pupae) of 36.67 and 40.00 respectively. While it was 66.76 when treated with potato broth adults food with filtrate recorded high mortality rate reached to 90.00% after one week.
The present study includes the effect of the ethanolic and aqueous extract of Piper nigrum against the third instar of Culex pipiens molestus (Diptera:Culicidae) in different concentrations: 1, 1.5 ppm of ethanolic extracts of Piper nigrum causing 100% mortality of larvae in the second day of treatment, while the concentrations 5% of aqueous extracts of Piper nigrum caused 100% mortality of larvae in the second day of treatment. The concentration 2.5% caused 100% mortality of larvae after four days of treatment. The current study also detected the effect of 0.1% concentration of Piper nigrum aqueous extract. The effect of this concentratio
... Show MoreThe chemical composition of wastes of pressed grapes and found that the main components of wastes of pressed grapes, represented by the percentage of moisture was 6.47%, and the proportions are 3.71%. Either carbohydrates amounted to 85.77 %, either in fat models using petroleum ether Petroleum ether) was the increase of 0.27%. estimated the percentage of ash in the sampls was 3.78%, either fiber reached 69.47 %,, in addition to the test extracts towards the growth of seven types of bacteria, which included Bacillus subtilis, Bacillus cereus, Bacillus stearothermophilus, Escherishia coli, Staphylococcus aureus, Salmonella typhimurium and Pseudomonas fluorescens and yeast Candida albicans and Kluyveromyces marxianus, ( diffusion method ).
... Show MoreEleven species of parasitic insects were recovered from puparia of house fly Musca domestica L. developing in animal dung in Baghdad during 1985-1987. Of the parasites obtained, representatives were found in five families of Hymenoptera and one family of Coleoptera. The most prevalent parasites were Spalangia cameroni Perkins, S. nigroaenea Curtis and S. endius Walk. Average parasitism for the two year was 11.30 %, the highest number of parasitism occurred in May and October.
Pigeons have accompanied humans since ancient time because they are used as a source of food, pets, hobby, and religious symbols. Pigeons have shown high prevalence rate of infection with gastrointestinal helminths and protozoan. This study was conducted to evaluate the prevalence of parasitic infections in the domestic pigeon (Columba livia domestica) from October, 2017 to April, 2018, purchased from bird market of Zakho City, Kurdistan region. The samples were taken from 50 adult pigeons (28 males and 22 females). The birds were transferred to Parasitology Laboratory, Faculty of Science, Zakho University. In the laboratory, each bird was sacrificed and immediately the feather and skin of under wings, chest and the rest of the
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