The objective of this study was to isolate and identify the asparaginase-producing bacteria, then purify and characterize the enzyme in order to investigate their properties in the future. Fifteen local bacterial isolates were isolated from various sites in the city of Baghdad, identified by conventional morphological and biochemical procedures, and confirmed using vitek 2 methods, and submitted to primary screening processes for asparaginase production. For secondary screening, eight isolates with the greatest yellow zone ability on a specific solid medium were chosen. Bacillus sp. was reported to have the highest enzyme production (7.5 U/mg proteins). After 24 hours of incubation, submerged fermentation yielded optimal conditions for the production of L-asparaginase (L-ASNase) by the chosen isolate, with medium (2) serving as the optimal medium for production and fructose serving as the optimal source of carbon. In pH 6 at 40°C, Sephadex G-150 gel filtration chromatography was used to purify the enzyme. The final purification folds were increased by 2.5 times, resulting in an enzyme yield of 93.7%. It also showed the highest purified enzyme activity and stability was at 37°C. Also it revealed the highest activity and stability at pH 7.0 and pH 8.0 respectively. Enzyme lost activity when exposed to several metallic ions at concentrations of 1, 5, and 10 mM.
this research has discussed some social phenomena in lslamic society through the book of the unique contract of lbn abed rabbi al-andalusi. ln the beginning , he gave an account of his life.
Parmi les oeuvres d'Albert Camus, qui s'éloignent un peu de sa
tendance générale qui traite les thèmes de l'absurde ,du néant et de la
question de la mort , on trouve son recueil "l'Exil et le Royaume" 1957
qui contient six nouvelles qui sont d'une invention moins épouvantable
(le Renégat , la Femme adultère , les Muets , le Pierre qui pousse , l'Hôte
et Jonas). Ces nouvelles , à l'exception du "Renégat" , ne provoquent pas
les questions philosophiques et dialectiques qui dominent la plupart des
oeuvres d'Albert Camus , Mais ces nouvelles traitent tout simplement la
question d'homme exilé dans son existence et sa recherche perpétuelle
de son royaume.
The research seeks to identify the contemporary events that face the use of electronic payment methods to localize the salaries of state employees and its impact in enhancing the mental image of customers, and to achieve this purpose from the fact that a questionnaire was designed and distributed to an optional sample of (31) individual customers (employees) dealing With the researched private banks, it has been analyzed and reached a number of conclusions and recommendations, the most prominent of which is the lack of modernity of electronic payment methods by customers, which is reflected in the mental image of customers and the achievement of their satisfaction, in the Emiratization project for salaries needs an advanced leade
... Show Moreمن نحن ؟ التحديات التي تواجه الهوية الامريكية
Summary:
We are aware that we cannot reach a correct level of understanding of the Arabic language without using the Quran as our main source in our research. Since the word of the Quran that put an end to all words which kneeled before it all the phrases that lead to the showcasing of the Arabic language in its best self.
Arab scientist realized this truth and gave all their attention to the singular and created from it an important subject for their research in the origins of the language and the science behind. Thus, since they dealt with the structure and studied the significance of the context through the use of vocabulary. Therefore, researching Islamic words or the “surat” mentioned in the Qur’an was necessar
Anger is one of the problems of scientific importance that psychologists and education scientists are interested in, especially societies and educational environments, because if a child’s anger continues to develop into violence, then it becomes an unusual behavior, and an indication of the child's lack of adaptation to his family and his environment (Moses, 2013: 4) &n
... Show Moreتصنيع الطابوق الحراري الثقيل من خامات محلية
اتخذت الهند موقفاً محايداً من الحرب الروسية الأوكرانية، لما له من أبعاد سياسية واقتصادية وعسكرية مع أطراف الصراع في الحرب، وقناعتها بعدم قدرتها على التأثير فيها. فهي تربطها علاقات استراتيجية تاريخية مع روسيا، الداعمة لها سياسياً في مجلس الأمن والوسيط ضد خصومها الصين وباكستان. لذلك الهند مقتنعة بأن أوكرانيا تُعدّ مجالاً للنفوذ الروسي الذي عمل حلف شمال الأطلسي على التوسع به رغم التحذيرات الروسية. لذا أيقنت ا
... Show MoreEtude de I' espace dans un extrait de Les sequestres d' Altona de Jean
The current research tries to study the reasons for lying since its
inception, as it is interesting to a child's moral growth, which can benefit from
the results of this research by institutions of socialization, parents and
teachers in the treatment and care of children.
The objective of this research is to highlight the reasons for lying to the
children from the viewpoint of teachers and the answer to the paragraphs of
research designed research instrument (questionnaire) was distributed to a
sample of teachers was clear from the analysis that the reasons lie that
received a high percentage of members of the sample are: -
- The fear of severe punishment
- Child rid himself of a specific problem
- To sat