Preferred Language
Articles
/
bsj-6842
A Developed Colorimetric Assay Using Unmodified Gold Nanoparticles for the Identification of Acinetobacter baumannii Isolates from Different Clinical Samples
...Show More Authors

  Acinetobacter baumannii (A. baumannii ) is considered a critical healthcare problem for patients in intensive care units due to its high ability to be multidrug-resistant to most commercially available antibiotics. The aim of this study is to develop a colorimetric assay to quantitatively detect the target DNA of A. baumannii based on unmodified gold nanoparticles (AuNPs) from different clinical samples (burns, surgical wounds, sputum, blood and urine). A total of thirty-six A. baumannii clinical isolates were collected from five Iraqi hospitals in Erbil and Mosul provinces within the period from September 2020 to January 2021. Bacterial isolation and biochemical identification of isolates were carried out followed by DNA extraction from 36 isolates and six negative ATCC strains (Salmonalle typhi, Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa, Enterobacter aeruginosa, Staphylococcus aures) and only one positive control ATCC A. baumannii using Phenol/Chloroform method. AuNPs were synthesized using the citrate reduction method and examined by XDR, FTIR, UV-VIS, FE-SEM, and TEM.  The optimized colorimetric assay was employed based on unmodified spherical AuNPs and PCR amplification of 16S rRNA intergenic spacer sequences (ITS) with species-specific DNA oligo-targeters. Detection and optimization of A. baumannii amplicons using unmodified AuNPs were performed based on species-specific DNA oligonucleotide. The AuNPs assay was able to colorimetrically detect and distinguish A. baumannii from other ATCC bacterial isolates. The turnaround time of this assay was about 3 hours, including sample preparation and amplification, to show (0.025-6 ngµl-1) as a detection limit of DNA concentration. The efficacy of colorimetric detection was proved to effectively diagnose A. baumannii isolates with high sensitivity, simplicity, and robustness to rapidly diagnose A. baumannii isolates from different clinical samples.

Scopus Crossref
View Publication Preview PDF
Quick Preview PDF
Publication Date
Wed Sep 01 2010
Journal Name
Journal Of Economics And Administrative Sciences
Using simulation to estimate parameters and reliability function for extreme value distribution
...Show More Authors

   This study includes Estimating scale parameter, location parameter  and reliability function  for Extreme Value (EXV) distribution by two methods, namely: -
- Maximum Likelihood Method (MLE).
- Probability Weighted Moments Method (PWM).

 Used simulations to generate the required samples to estimate the parameters and reliability function of different sizes(n=10,25,50,100) , and give real values for the parameters are and , replicate the simulation experiments (RP=1000)

... Show More
View Publication Preview PDF
Crossref
Publication Date
Sun Feb 28 2021
Journal Name
Journal Of Economics And Administrative Sciences
Using jack knife to estimation logistic regression model for Breast cancer disease
...Show More Authors

 

It is considered as one of the statistical methods used to describe and estimate the relationship between randomness (Y) and explanatory variables (X). The second is the homogeneity of the variance, in which the dependent variable is a binary response takes two values  (One when a specific event occurred and zero when that event did not happen) such as (injured and uninjured, married and unmarried) and that a large number of explanatory variables led to the emergence of the problem of linear multiplicity that makes the estimates inaccurate, and the method of greatest possibility and the method of declination of the letter was used in estimating A double-response logistic regression model by adopting the Jackna

... Show More
View Publication Preview PDF
Crossref
Publication Date
Sun Aug 01 2021
Journal Name
Telkomnika (telecommunication Computing Electronics And Control)
Online 3D path planning for Tri-copter drone using GWO-IBA algorithm
...Show More Authors

View Publication
Scopus (4)
Crossref (1)
Scopus Crossref
Publication Date
Sat Dec 05 2015
Journal Name
PrzeglĄd Elektrotechniczny
Power Amplifier Frequency Controller Using feedback control techniques for Bio-implanted Devices
...Show More Authors

View Publication
Scopus Crossref
Publication Date
Wed Feb 01 2023
Journal Name
Indonesian Journal Of Electrical Engineering And Computer Science
Diagnose COVID-19 by using hybrid CNN-RNN for Chest X-ray
...Show More Authors

<p>Combating the COVID-19 epidemic has emerged as one of the most promising healthcare the world's challenges have ever seen. COVID-19 cases must be accurately and quickly diagnosed to receive proper medical treatment and limit the pandemic. Imaging approaches for chest radiography have been proven in order to be more successful in detecting coronavirus than the (RT-PCR) approach. Transfer knowledge is more suited to categorize patterns in medical pictures since the number of available medical images is limited. This paper illustrates a convolutional neural network (CNN) and recurrent neural network (RNN) hybrid architecture for the diagnosis of COVID-19 from chest X-rays. The deep transfer methods used were VGG19, DenseNet121

... Show More
View Publication
Scopus (17)
Crossref (2)
Scopus Crossref
Publication Date
Tue Nov 01 2022
Journal Name
2022 International Conference On Data Science And Intelligent Computing (icdsic)
An improved Bi-LSTM performance using Dt-WE for implicit aspect extraction
...Show More Authors

In aspect-based sentiment analysis ABSA, implicit aspects extraction is a fine-grained task aim for extracting the hidden aspect in the in-context meaning of the online reviews. Previous methods have shown that handcrafted rules interpolated in neural network architecture are a promising method for this task. In this work, we reduced the needs for the crafted rules that wastefully must be articulated for the new training domains or text data, instead proposing a new architecture relied on the multi-label neural learning. The key idea is to attain the semantic regularities of the explicit and implicit aspects using vectors of word embeddings and interpolate that as a front layer in the Bidirectional Long Short-Term Memory Bi-LSTM. First, we

... Show More
View Publication Preview PDF
Scopus (6)
Crossref (5)
Scopus Crossref
Publication Date
Thu Apr 20 2023
Journal Name
Fire
An Efficient Wildfire Detection System for AI-Embedded Applications Using Satellite Imagery
...Show More Authors

Wildfire risk has globally increased during the past few years due to several factors. An efficient and fast response to wildfires is extremely important to reduce the damaging effect on humans and wildlife. This work introduces a methodology for designing an efficient machine learning system to detect wildfires using satellite imagery. A convolutional neural network (CNN) model is optimized to reduce the required computational resources. Due to the limitations of images containing fire and seasonal variations, an image augmentation process is used to develop adequate training samples for the change in the forest’s visual features and the seasonal wind direction at the study area during the fire season. The selected CNN model (Mob

... Show More
View Publication
Scopus (23)
Crossref (23)
Scopus Clarivate Crossref
Publication Date
Thu Jan 06 2022
Journal Name
Kuwait Journal Of Science
AVO analysis for high amplitude anomalies using 2D pre-stack seismic data
...Show More Authors

Amplitude variation with offset (AVO) analysis is an 1 efficient tool for hydrocarbon detection and identification of elastic rock properties and fluid types. It has been applied in the present study using reprocessed pre-stack 2D seismic data (1992, Caulerpa) from north-west of the Bonaparte Basin, Australia. The AVO response along the 2D pre-stack seismic data in the Laminaria High NW shelf of Australia was also investigated. Three hypotheses were suggested to investigate the AVO behaviour of the amplitude anomalies in which three different factors; fluid substitution, porosity and thickness (Wedge model) were tested. The AVO models with the synthetic gathers were analysed using log information to find which of these is the

... Show More
View Publication
Scopus (2)
Crossref (1)
Scopus Clarivate Crossref
Publication Date
Mon Feb 18 2019
Journal Name
Iraqi Journal Of Physics
Automated method for buried object detecting using ground penetrating radar (GPR) survey
...Show More Authors

  Ground Penetrating Radar (GPR) is a nondestructive geophysical technique that uses electromagnetic waves to evaluate subsurface information. A GPR unit emits a short pulse of electromagnetic energy and is able to determine the presence or absence of a target by examining the reflected energy from that pulse. GPR is geophysical approach that use band of the radio spectrum. In this research the function of GPR has been summarized as survey different buried objects such as (Iron, Plastic(PVC), Aluminum) in specified depth about (0.5m) using antenna of 250 MHZ, the response of the each object can be recognized as its shapes, this recognition have been performed using image processi

... Show More
View Publication Preview PDF
Crossref
Publication Date
Sun Jun 07 2015
Journal Name
Baghdad Science Journal
Optimum conditions for Inulinase production by Aspergillus niger using solid state fermentation
...Show More Authors

Thirty local fungal isolates according to Aspergillus niger were screened for Inulinase production on synthetic solid medium depending on inulin hydrolysis appear as clear zone around fungal colony. Semi-quantitative screening was performed to select the most efficient isolate for inulinase production. the most efficient isolate was AN20. The optimum condition for enzyme production from A. niger isolate was determined by busing a medium composed of sugar cane moisten with corn steep liquor 5;5 (v/w) at initial pH 5.0 for 96 hours at 30 0C . Enzyme productivity was tested for each of the yeast Kluyveromyces marxianus, the fungus A. niger AN20 and for a mixed culture of A. niger and K. marxianus. The productivity of A. niger gave the highest

... Show More
View Publication Preview PDF
Crossref (3)
Crossref