Crop diseases are usually caused by inoculum of pathogens which might exist on alternate hosts or weeds as endophytes. These endophytes, cum pathogens, usually confer some beneficial attributes to these weeds or alternate hosts from protection against herbivores, disease resistance, stress tolerance to secondary metabolites production. This study was therefore carried out to isolate potential crop pathogens which exist as endophytes on weed species in the University of Ilorin plantations. Green asymptomatic leaves were collected from 10 weed species across the plantations, and processed for their endophytic fungi isolation. Isolates were purified into pure cultures and used for molecular identification using the internal transcribed spacer (ITS) region of the ribosomal DNA. Phylogenetic analysis of the fungal sequences using MEGA software revealed 9 fungal genera belonging to 13 species, with species in the genera Curvularia, Epicoccum and Daldinia occurring in more than one weed species, while other genera such as Alternaria, Fusarium, Chaetomium, Macrophomina, Arthrinium and Phomopsis occurred in just one weed species each. Daldinia eschscholtzii was isolated in this study as an endophyte from Loudetia arundinacea for the first time. This plant is very abundant in Nigeria and Africa where it is used majorly for thatching and feeding livestocks. This also represents the first endophytic fungi from the genus Loudetia. Potential relationship between the occurrences of these fungi as endophytes and as pathogens are discussed. These discoveries represent the first large-scale molecular identification and several first reports of endophytes from these weed species. These results also represent the first records of some of these fungi in Nigeria.
The current study aims to determine the prevalence of Trichomonas vaginalis and Candida spp., and also to identify Candida parapsilosis and some virulence genes. It was conducted in Bint Al-Hoda Hospital of Maternity and Children in Thi-Qar province, south of Iraq for the period from the beginning of January to the end of December 2020. Two hundred and fifty samples were collected from the female genital tract for women whose age ranged between 17-50 years. Microscopic, traditional and molecular tests were used in the sample examination. The results recorded 12 (4.8%) samples infected with T. vaginalis parasite, whereas 130 (52%) samples showed Candida yeast distributed as follows: 75 (30 %) <
... Show MoreBackground: Candida albicans is the principal fungal infectious agent in human infection. Adhesion is thought to be an essential step for colonization and establishment of Candida infections.
Objectives: Identification and comparison of ALS1 virulence gene of adhesion family among different isolates of Candida albicans by PCR.
Patients and methods: One hundred eight samples were collected from different group of Iraqi patients. All samples were culture on Sabouraud′s agar, CHROMagar for identification while API Candida kit confirmatory test and extracted DNA was done for just Candida albicans isolates, detected the ALS1 gene, extracted RNA for synthesis of cDNA and detected of gene and compare between iso
Accounting disclosure is the main means and effective tool for communicating business results to users in support of their decisions, especially those with thought and specialization from academics and professionals in the field of accounting and auditing about the importance of accounting disclosure and transparency in financial reports.
Contingent liabilities represent commitments based on the occurrence of one or more events in the future to confirm the value due, the party entitled to it, the maturity date, or to confirm the existence of the obligation itself, and therefore they should not be recognized as a contingent liability i
... Show MoreResistance to aminoglycosids is a great problem to therapeutics. Aminoglycoside acetyltransferase producing Pseudomonas aeruginosa have been reported to be important cause of nosocomial infections. The purpose of this study was to determine the occurrence of aminoglycoside acetyltransferase. A total of 200 clinical and environmental samples were collected over period of five months. The P. aeruginosa isolates were confirm their identification, antibiotic susceptibility profile according to vitek2 compact system. The isolates were subjected to polymerase chain reaction (PCR) assays with specific primers for aac (6')-I, aac (6')-Ib, aac (3')-I . Only 32 (16.%) P. aeruginosa isolates were recovered from the samples. in present investigation
... Show MoreThe prostaglandins inside inflamed tissues are produced by cyclooxygenase-2 (COX-2), making it an important target for improving anti-inflammatory medications over a long period. Adverse effects have been related to the traditional usage of non-steroidal anti-inflammatory drugs (NSAIDs) for the treatment of inflammation, mainly centered around gastrointestinal (GI) complications. The current research involves the creation of a virtual library of innovative molecules showing similar drug properties via a structure-based drug design. A library that includes five novel derivatives of Diclofenac was designed. Subsequently, molecular docking through the Glide module and determining the binding free energy implementing the P
... Show MoreTen new thiourea derivatives 1-10 were prepared in this work using a two-step process that involved reacting 4-methoxybenzoyl chloride with KSCN to afford 4-methoxybenzoyl isothiocyanate. This was followed by reaction with various amines (primary amines, secondary amines, and diamines) to give the aforementioned title products 1-10. These products were characterized by FT-IR, 1H NMR and 13C NMR spectroscopy. Using the DPPH scavenging method, the antioxidant activity of thiourea products was investigated, and derivative 8 had the greatest antioxidant activity in comparison to the other derivatives. Moreover, molecular dockin
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