A significant increase in the incidence of non-O157 verotoxigenic Escherichia coli (VTEC) infections have become a serious health issues, and this situation is worsening due to the dissemination of plasmid mediated multidrug-resistant microorganisms worldwide. This study aims to investigate the presence of plasmid-mediated verotoxin gene in non-O157 E. coli. Standard microbiological techniques identified a total of 137 E. coli isolates. The plasmid was detected by Perfectprep Plasmid Mini preparation kit. These isolates were subjected to disk diffusion assay, and plasmid curing with ethidium bromide treatment. The plasmid containing isolates were subjected to a polymerase chain reaction (PCR) for investigating the presence of plasmid mediated verotoxin gene (VT1 and VT2) in non-O157 E. coli. Among the 137 E. coli isolates, 49 isolates were non-O157 E. coli while 29 (59.1%) isolates were verotoxin producing non-O157 serotypes and 26 non-O157 VTEC isolates possessed plasmids. Certain isolates harboured single sized plasmid while others had multiple plasmids with different size varied from 1.8kb to 7.6kb. A plasmid containing all (100%) the isolates was multidrug-resistant. Eight isolates changed their susceptibility patterns while three isolates were found to lose plasmid after post plasmid curing treatment and the rest of the isolates (15) remained constant. Different PCR sets characterized 3 plasmid-mediated verotoxins producing non-O157 E. coli. This current study demonstrated the occurrence of plasmid mediated verotoxin gene in non-O157 E. coli. To the best of our knowledge, this is the first report in the global literature on plasmid-mediated verotoxin gene in non-O157 E. coli. Timely diagnosis and surveillance of VTEC infections should prioritize to stop or slow down the virulence gene for dissemination by plasmid-mediated gene transfer amongst the same bacteria or other species.
Since God created human and had him live on Earth, he was certain and focused on marriage and having lineage for the purpose of development of this Earth which he – God - provided for humans.
We notice that he – human - was interested in curing disease of the genital system, (whether male and female) and he used different methods for that and what is available in every era and periods, we saw him exceeded in using different medical Herbs in different area of Earth and used it efficiently for healing and getting the best results they even inherited from one generation to another, and they didn't even stop here,
ABSTRUCT
This research aims at examining the expected gap between the fact of planning and controlling process of production at the State Company for Electric Industries and implementation of material requirements planning system in fuzzy environment. Developing solutions to bridge the gap is required to provide specific mechanisms subject to the logic of fuzzy rules that will keep pace with demand for increased accuracy and reduced waiting times depending on demand forecast, investment in inventory to reduce costs to a minimum.
The proposed solutions for overcoming the research problem has required some questions reflecting the problem with its multiple dimensions, which ar
... Show MoreRecent growth in transport and wireless communication technologies has aided the evolution of Intelligent Transportation Systems (ITS). The ITS is based on different types of transportation modes like road, rail, ocean and aviation. Vehicular ad hoc network (VANET) is a technology that considers moving vehicles as nodes in a network to create a wireless communication network. VANET has emerged as a resourceful approach to enhance the road safety. Road safety has become a critical issue in recent years. Emergency incidents such as accidents, heavy traffic and road damages are the main causes of the inefficiency of the traffic flow. These occurrences do not only create the congestion on the road but also increase the fuel consumption and p
... Show MoreThis paper proposes a new method Object Detection in Skin Cancer Image, the minimum
spanning tree Detection descriptor (MST). This ObjectDetection descriptor builds on the
structure of the minimum spanning tree constructed on the targettraining set of Skin Cancer
Images only. The Skin Cancer Image Detection of test objects relies on their distances to the
closest edge of thattree. Our experimentsshow that the Minimum Spanning Tree (MST) performs
especially well in case of Fogginessimage problems and in highNoisespaces for Skin Cancer
Image.
The proposed method of Object Detection Skin Cancer Image wasimplemented and tested on
different Skin Cancer Images. We obtained very good results . The experiment showed that
Vatan ve hürriyet aşkının ilk ve en Kuvvetli örneklerinden Türkmen topraklarında yetişen yüzlerce Şairlerden biri Hıdır Lütfü’dür. Düşmana yar, öz evladına ağyar, yumuşak tabiatlı, yardımı ve milletini seven bir insandır. Şairin duygusu her dem yükseklere yücelirdi. O bilgili bir şair olduğu için şiirinde türlü türlü bilgi ile ögütleri arkasında bırakmamış onun duygusu bir bulak gibi her dem durmadan coşardı.
Onun en çok sevdiği varlıklar yurdu ile ulusuydı, ve tek acısı onların ilerleme ile yükselemesi içın bir haykırıydı.
Şair her zaman yurdunun ufukunda dolaşıp ulusunun bugünkü ve geleck torunları için yanıklı duyguları ile bir yol gosterıci gibi olmuştur.
- The study aimed to identify the position of the university leaders from the proposed abolition of the Ministry of Higher Education in Jordan as un approach for the development of society, the study relied descriptive method survey with the assistance consists of (33) items, and check validity and reliability of the instrument, and was applied to a sample of university leaders, (57) a leading university presidents and their assistants and deans and their assistants, and after processing the data bagful statistical( spss) overall results showed the to arithmetic average of (2.74) and the degree of medium and this means that most respondents moderately support the abolition of the ministry, the most prominent item that makes
הסיכום
הסיפורת העברית באמריקה, הייתה קשורה בסביבה מסויימה. גם כן היא קשורה בנושאים מסויימים, ורובם היו מדברים על חיי היהודים באמריקה.
דמותו של שמעון הלקין קבועה בנפשם של המבקרים כזו של משורר בעי
... Show Moreأرمينيا أكبر وأعلى إقليم جبلي في آسيا الغربية ، تقع عند الحدود الفاصلة بين آسيا وأوربا ، انعمت عليها الطبيعية بتضاريس كانت تارة خيراً وبركة على الأرمن وتارة اخرى شراً ووبالاً عليهم ، والى جانب كونها منطقة عبور هامة بين الشرق والغرب فان الارباح الطائلة التي جنتها من نشاطها الاقتصادي دفع الدول الكبرى آنذاك إلى السيطرة عليها وسلبها حريتها . وتتابع على ضمها الى نفوذه منذ ظهورها في القرن السابع قبل الميلاد كل من ا
... Show MoreThis study aimed to determine the extent matching his chemistry for fourth grade scientific quality standards, from the point of view of male and female teachers. The study sample consisted of (96) as a teacher and school representing schools six Baghdad educations (Rusafa - Karkh), and search tool consisted of identifying closed, accounting standards and specifications should be available in a chemistry book, after it has been ascertained from the sincerity and persistence. The percentage used Pearson correlation coefficient and the Fisher equation and weight percentile, as a means to describe the statistical analysis and interpretation of the results that have been reached. The results revealed that the percentage of matching s
... Show MoreWhat a nation seeking to occupy a prominent place among the nations not and attached the educational process of the great interest from which you can build a conscions generation represented in the culture first, then unable to adapt to modern technology data on the labor market second .
So it was for the administration a major role in the educational process and that all the evaluation of texture development of education in his administration , it is through the development of administrative paper work can be judged or under developed society , evaluation of any system depends on the efficiency of his administration in dealing with the inputs of the education system.
... Show More