Preferred Language
Articles
/
bsj-3895
Molecular Characterization of Plasmid-Mediated Non-O157 Verotoxigenic Escherichia coli Isolated from Infants and Children with Diarrhea
...Show More Authors

A significant increase in the incidence of non-O157 verotoxigenic Escherichia coli (VTEC) infections have become a serious health issues, and this situation is worsening due to the dissemination of plasmid mediated multidrug-resistant microorganisms worldwide. This study aims to investigate the presence of plasmid-mediated verotoxin gene in non-O157 E. coli. Standard microbiological techniques identified a total of 137 E. coli isolates. The plasmid was detected by Perfectprep Plasmid Mini preparation kit. These isolates were subjected to disk diffusion assay, and plasmid curing with ethidium bromide treatment. The plasmid containing isolates were subjected to a polymerase chain reaction (PCR) for investigating the presence of plasmid mediated verotoxin gene (VT1 and VT2) in non-O157 E. coli. Among the 137 E. coli isolates, 49 isolates were non-O157 E. coli while 29 (59.1%) isolates were verotoxin producing non-O157 serotypes and 26 non-O157 VTEC isolates possessed plasmids. Certain isolates harboured single sized plasmid while others had multiple plasmids with different size varied from 1.8kb to 7.6kb. A plasmid containing all (100%) the isolates was multidrug-resistant. Eight isolates changed their susceptibility patterns while three isolates were found to lose plasmid after post plasmid curing treatment and the rest of the isolates (15) remained constant. Different PCR sets characterized 3 plasmid-mediated verotoxins producing non-O157 E. coli. This current study demonstrated the occurrence of plasmid mediated verotoxin gene in non-O157 E. coli. To the best of our knowledge, this is the first report in the global literature on plasmid-mediated verotoxin gene in non-O157 E. coli. Timely diagnosis and surveillance of VTEC infections should prioritize to stop or slow down the virulence gene for dissemination by plasmid-mediated gene transfer amongst the same bacteria or other species.

Scopus Clarivate Crossref
View Publication Preview PDF
Quick Preview PDF
Publication Date
Tue Feb 27 2024
Journal Name
مؤسسة دار الصادق الثقافية
الجامعة ودورها في الحد من تعاطي المخدرات وتأثيرها على المدمنين (اسباب وحلول ومعالجات)
...Show More Authors

ملخص البحث: تعتبر مشكلة تعاطي المخدرات من المشكلات التي تؤثر في بناء المجتمع وأفراده لما يترتب عليها من آثار اجتماعية واقتصادية ونفسية سيئة تنسحب على الفرد و على المجتمع، كما أنها ظاهرة اجتماعية مرضية تدفع إليها عوامل عديدة؛ بعضها يتعلق بالفرد والبعض الآخر بالأسرة والثالث بالبناء الاجتماعي ككل. وتعد ظاهرة انتشار المخدرات من الظواهر الأكثر تعقيدا وبارزة هذه الظاهر ة وإحدى مشكلات العصر، الشاملة في المجتمع، و

... Show More
Publication Date
Fri Feb 08 2019
Journal Name
Journal Of The College Of Education For Women
الأسطول والقوة البحریة في سلطنة عُمان خلال النصف الأول من القرن التاسع عشر
...Show More Authors

Development and progress of the fleet and the navy in Oman during
the reign of Sultan Said bin sultan from 1804 to 1856.
It had second level in Arab Gulf and Indian Ocean during the first half
of nineteenth century. The fleet of Oman in this time became after British
fleet.
Several reasons were behind the development of Omani fleet
- First : The aim of Sultan Said was to built a large navy yard in Arab Gulf
and Indian Ocean to encourage him -to made many ships factories.
- Second: Arab Gulf and Indian Ocean within the reign of Sultan Said
wittinsed the confilicts and wars between different forces it drived Sultan Said
to built agood and strong navy.
- Third The main aim of Oman during the reign of Sultan

... Show More
View Publication Preview PDF
Publication Date
Wed May 01 2019
Journal Name
المجلة العربية للعلوم التربوية والنفسية
الصحة النفسية وعلاقتها بتقدير الذات لدى عينة من أطفال الشوارع في مدينة بغداد
...Show More Authors

هدف البحث إلى الکشف عن العلاقة بين الصحة النفسية وتقدير الذات لدى أطفال الشوارع، تألفت عينة البحث من (50) طفلاَ وطفلة، وتم تطبيق مقياسا البحث - مقياس الصحة النفسية ومقياس تقدير الذات وهما (من إعداد الباحثة)، وقد اسفرت نتائج البحث عن إن أطفال الشوارع اظهروا مستوى منخفض في الصحة النفسية وکذلک الحال بالنسبة لتقدير الذات، فضلاً عن وجود علاقة ارتباطية موجبة دالة إحصائيا بين الصحة النفسية وتقدير الذات. وفي ضوء نتائج

... Show More
View Publication Preview PDF
Publication Date
Fri Feb 08 2019
Journal Name
Journal Of The College Of Education For Women
المتغیرات الداخلیة والخارجیة المؤثرة على النظام السیاسي في باكستان ٢٠٠٩ - للمدة من ١٩٩
...Show More Authors

The research(the internal and external factors affecting the political system in
PAKISTAN) involves a series of effects interact both internally and external to affect the
stsbility of the political system.
Intrernally there are a lot of powers affecting the political map of PAKISTAN such as
military institution, political parties, judgement, religious schools……etc.of internal
factors, which represent one of the most important pressure points on the system.
Externally one of the most important external factors which affect internal stability is
Pakistani allision to USA in antiterrorism war. The Pakistani military institution was
involved in the war against Pakistani tribes leading to unstable security in Paki

... Show More
View Publication Preview PDF
Publication Date
Sun Jan 01 2012
Journal Name
Journal Of The College Of Languages (jcl)
המאבק הפנימי של מרדכי זאב פיארברג מתוך גיבורו של הסיפור "לאן?"
...Show More Authors

המחקר הזה עוסק באחד מהסופרים העבריים שנחשבו בעלי־השפעה בתהליך הספרות העברית החדשה על אף גילו הקצר ועל אף שהוא השאיר עזבון ספרותי מועט, אך עם זה דמותו עולה לענינו גם מתוך כתביו המעטים כאילו הם טבועים בחותם, הסופר הזה הוא מרדכי זאב פיארברג.

 

            במחקר זה טיפלנו בחייו של המספר הזה בקיצור וגם ביצירותיו באופן כללי, ובסימנים המאפינים את גיבוריו, ובמיוחד גיבורו של הסיפור "

... Show More
View Publication Preview PDF
Publication Date
Sun Sep 01 2019
Journal Name
Al-khwarizmi Engineering Journal
Fuzzy Analytical Hierarchy Process for Embedded Risk Reduction in Selecting the Right Planning Decision
...Show More Authors

The aim of this work is to provide an efficient selection technique as a part of planning process to guide the decision makers to decide the preferences of one supplier over another for purchasing lab instruments in education domain. Fuzzy Analytical Hierarchy Process has used as a multi-criteria decision process, as an industrial engineering tool with certain emphasis on the qualitative aspects required to the decision makers. While the concept of degree of possibility for each criterion is used to reach its relative weights, a specific methodology created to reach the final objective decision of supplier selection. A questionnaire form was developed and distributed to five universities located in Baghdad province with a total

... Show More
View Publication Preview PDF
Publication Date
Fri Dec 01 2023
Journal Name
Baghdad Science Journal
Solving the Hotdog Problem by Using the Joint Zero-order Finite Hankel - Elzaki Transform
...Show More Authors

This paper is concerned with combining two different transforms to present a new joint transform FHET and its inverse transform IFHET. Also, the most important property of FHET was concluded and proved, which is called the finite Hankel – Elzaki transforms of the Bessel differential operator property, this property was discussed for two different boundary conditions, Dirichlet and Robin. Where the importance of this property is shown by solving axisymmetric partial differential equations and transitioning to an algebraic equation directly. Also, the joint Finite Hankel-Elzaki transform method was applied in solving a mathematical-physical problem, which is the Hotdog Problem. A steady state which does not depend on time was discussed f

... Show More
View Publication Preview PDF
Scopus Crossref
Publication Date
Sun Jul 04 2010
Journal Name
Journal Of Educational And Psychological Researches
الأنماط القيادية السائدة لدى مديري المدارس المتوسطة من وجهة نظر أعضاء الهيئة التدريسية
...Show More Authors

تعتبر المدرسة المتوسطة هي القاعدة التعليمية الثانية بعد المدرسة الابتدائية والتي تقوم عليها أنظمة التعليم المتنوعة فهي تمثل اللبنة الثانية في الصرح التعليمي لأنها مرحلة مهمة تستقبلها الطلبة في العمر الحرج المليء بالتطورات الجديدة على المستويات الثلاثة البايلوجي والنفسي والاجتماعي وعليه يأتي هنا دور القيادة التربوية للقيام بواجباتهم الإدارية والقيادية في قمة الهرم الإداري للمدرسة المتوسطة والتي تستوج

... Show More
View Publication Preview PDF
Publication Date
Sun Nov 04 2018
Journal Name
Arab Science Heritage Journal
آل الاشعث آل الأشعث وموقفهم من مقتل الخليفة علي وولده الحسن (عليهما السلام)
...Show More Authors

The research position ofAl-A'shath from ahlulbayt lies theimportance of research to clarify the exten of resentment and hatred which carries these owners of family household and the reason for hatred and animosity dates bach to his place and the hom of the people of the house of god and the prophet and the people the research is divided in to introduction and tow section and the ring included afirstsectionA'shath bin Qais position in the murderof caliph ali the second section has included the role of the girl crumple A'shath in the venom of imam Hassan and the bottom line is that the house  of A'shath proceeded to such acts of the door of hatred and resentment and hostility lal house.

View Publication Preview PDF
Publication Date
Sun Apr 03 2011
Journal Name
Journal Of Educational And Psychological Researches
اتجاهات مديرات الرياض نحو الاساليب التربوية المتبعة من قبل المعلمات مع اطفال الروضة
...Show More Authors

The aim of the research to show the direction of kindergarten‘s
headmasters with students and know the educational technique used by
the teachers with students inside kindergarten , also to know the
differences of headmasters directions towards these technique
according to changing of social state (married , unmarried ) and age
state (30-38) (39-47) (48-56) .
Also shows the sign among the direction of head masters and
educational technique used by teachers with student, where as the
importance of this research shows the importance of the kindergarten
stage and the importance of management .
The role of headmaster and teacher consider the relationship between
them and the mother so that this origin direc

... Show More
View Publication Preview PDF