Fever is a common illness in the pediatric age group ,the causes could be viral ,bacterial and fungal , this study was focused on bacterial pathogens as gram positive like Staphylococci, coagulase positive or negative ,Streptococci and gram negative like E-coli , Klebsialla ,Proteus, Pseudomonas, Burkhoderia , Acinetobacter and others like Pusturella ,E-alkalescendiaper, Haemophillus influenza and yeast like candida . Four thousand and seventy eight blood samples (4078)were collected in a period between January 2011 and the end of May 2012 at the child welfare hospital ,all the samples were cultured on suitable culture media and then biochemical tests were done using API-E 20 and sugar fermentation tests ,sensitivity test were done with number of antibiotics [1]. 4078 cases examined only ,1107 showed positive growth ,264cases were contaminated and no bacterial growth seen in 2707. The bacterial isolates during one year in pediatric age groups were mostly gram positive cocci ,followed by gram negative bacilli,and yeasts .
The aim of the research is to determine the extent to which young people watch television sports programs and the impact of viewing on the level of sports culture among young people and the impact of demographic variables on youth over the viewing of sports programs, The study was conducted on a sample of (200) male and female students from the faculties of the University of Baghdad, where a questionnaire form composed of several axes and questions was prepared to measure the effectiveness of sports programs in youth culture, The statistical program used spss to empty data and extract results by frequency, percentages, correlations, The research reached several results, the most important of which is that there is a strong influence of s
... Show MoreBackground: Bacteriocin is a peptidic toxin has many advantages to bacteria in their ecological niche and has strong antibacterial activity. Objective: The aim of this study was to evaluation of bacteriocin using Streptococcus sanguinis isolated from human dental caries.
Subjects and Methods: Thirty five streptococcus isolates were diagnosed and tested for their production of bacteriocin, and then the optimal conditions for production of bacteriocin were determined. After that, the purification of bacteriocin was made partially by ammonium sulfate at 95% saturation levels, followed by and gel filtration chromatography
... Show MoreSamples of the root nodules were collected to isolate different species of the genus Rhizobium from several leguminous plants; Trigonella foenum-graecum, Medicago sativa, Lens culinaris, Vigna mungo, Vicia faba, Phaseolus vulgaris, and Cicer arietinum, and based on their morphological, cultural, and biochemical characteristics, in addition to the identification of each isolate at the species level by amplified polymerase chain reaction (PCR) and using the sequencing of the nitrogenous bases of the 16S rRNA gene, it was identified as Sinrhizobium meliloti, Sinrhizobium meliloti, Bradyrhizobium elkanii, Rhizobium leguminosarium biovar viciae, Rhizobium leguminosarium biovar phaseoli and Mesorh
... Show MoreNanotechnology is a continually expanding field for its uses and applications in multiple areas i.e. medicine, science, and engineering. Biosynthesis is straightforward, less-toxicity, and cost-effective technology. TiO2 NPs biosynthesis has attained consideration in recent decades. In this study, probiotic bacteria were isolated from cow’s raw milk samples, and then were identified by using the Vitek2 system; as Leuconostoc spp. included Leuconostoc mesenteroides subsp. mesenteroides (Leu.1), Leuconostoc mesenteroides subsp. cremoris (Leu.4), and Leuconostoc pseudomesenteroides (Leu.14). All Leuconostoc spp. isolates showed an ability for TiO2 NPs bio-production, after being incubated at anaerobic conditions (30 o C/ 24 h) in DeM
... Show MoreBackground: Hydatosis caused by Echinococcus granulosus dog tap worm is zoonotic infection and economic importance and to public health constitutes a major threat in certain regions of the Middle East. There is an establishment of preventive and control strategy for characterization of E.granulosus in all endemic area which is essential in all molecular studies, to check the DNA of the parasite.
Objective: Our study aimed to obtain the best from three extractions DNA methods from hydatid cyst protoscolecses isolated from sheep in Al-shawlla abattoir in Baghdad.
Subjects and Methods: Three methods were used to extract DNA samples (boiling, crushing and commercial) DNA samples were performed with electrophoreses on 1.3% agarose. Rega
Microbiological contamination by fungi impacts the quality and safety of wheat grain storage. This study aimed to evaluate the efficacy of cold plasma in restricting the growth of the fungus, Aspergillus niger, which was isolated from wheat grains. A dielectric barrier discharge (DBD) operating at atmospheric pressure generated cold plasma that was used to treat the fungus, and the impact of this treatment was investigated at various periods 1, 2, 4, 6, and 15 minutes. The results revealed a highly significant decrease in the growth and number of spores of Aspergillus niger compared to the controls. This study revealed an efficient technique for enhancing wheat grain storage that could be a foundation for further large-scale studies.
... Show MoreThirty swabes of medical implants were collected from Al-Yarmouk's hospital which were cultured on manitole agar to isolate Staphelococcus aureus . Only four samples gave positive results with this media. It was used ten types of antibiotics to test the sensitivity of this bacterium against them. All isolates of S. aureus were recorded as multidrug resistant and were considered as MRSA. One pledge alternative therapy is the utilize of certain pure bacterocin MIC (32.5 to 62.5 μg/ml) and it was compared with vancomycin (200-400 μg/ml) with average of (8 – 15) mm diameter of inhibition zones recpectively. The first reduction of biofilm formation ability has been proved in catheters when treatedby pure bacterocin. The test shows the highes
... Show MoreThis study is aimed to Green-synthesize and characterize Al NPs from Clove (Syzygium aromaticum
L.) buds plant extract and to investigate their effect on isolated and characterized Salmonella enterica growth.
S. aromaticum buds aqueous extract was prepared from local market clove, then mixed with Aluminum nitrate
Al(NO3)3. 9 H2O, 99.9% in ¼ ratio for green-synthesizing of Al NPs. Color change was a primary confirmation
of Al NPs biosynthesis. The biosynthesized nanoparticles were identified and characterized by AFM, SEM,
EDX and UV–Visible spectrophotometer. AFM data recorded 122nm particles size and the surface roughness
RMs) of the pure S. aromaticum buds aqueous extract recorded 17.5nm particles s
One hundred specimens from wounds, burns, and skin swabs were collected
from patients laying and attended to Balad general hospital. It was found that 50
isolates belong to Staphylococcus spp., 38 isolates were identified as S. aureus and
12 isolates were identified as S. epidermidis according to microscopic, cultural and
biochemical testing. The study of seven extracellular enzyme as virulence factors
including the enzymes: urease, lipase, DNase, haemolysin, coagulase, β-lactamase,and lecithinase. Reavealed that 100% of S.aureus had the ability to produce these
enzymes, while S. epidermidis isolates were unable to produce the enzymes DNase,
lipase, coagulase, but they were capable to produce haemolysin, urease, lec