The ability of four local fungal isolates for extracellular laccase production has been tested with five grams 1:1(w/v) humidified sawdust as substrate in mineral salt medium. After 21 day of incubation at 25±1 ? C and using one mycelial plug (5mm), higher level of laccase activity (0.15U/ml) and specific activity (15U/mg) were observed by Pleurotus ostreatus in comparison with other fungal isolates. The results of optimum conditions for laccase production from selected isolate showed that, the maximum laccase activity (0.55U/ml) and specific activity (55U/mg) were obtained at moisture ratio 1:3 (w/v), using 3 mycelial plugs (5 mm), after 15 days incubation period at 25±1 ? C. The results of phenol degradation by crud laccase revealed th

Recent studies have proved the important role of fungi in the biodegradation of oil pollutants. The present study aims to find the optimal conditions for the fungi to get the best rate of the biodegradation of the polycyclic aromatic hydrocarbon (PAHs) (Naphthalene) compounds. Soil samples were taken from 18 different sites polluted with oil wastes and cultured then obtained 312 isolated fungi from 64 replicates Primarily screening were done on fungal isolates on solid media containing naphthalene the results revealed that 25 fungal isolates gave good growth, 47 fungal isolates gave Moderate growth, 66 gave weak growth and 147 fungal isolates gave no growth on Naphthalene solid media.
Then secondary screening were done on 25 fungal is

Recent studies have proved the important role of fungi in the biodegradation of oil pollutants. The present study aims to find the optimal conditions for the fungi to get the best rate of the biodegradation of the polycyclic aromatic hydrocarbon (PAHs) (Naphthalene) compounds. Soil samples were taken from 18 different sites polluted with oil wastes and cultured then obtained 312 isolated fungi from 64 replicates Primarily screening were done on fungal isolates on solid media containing naphthalene the results revealed that 25 fungal isolates gave good growth, 47 fungal isolates gave Moderate growth, 66 gave weak growth and 147 fungal isolates gave no growth on Naphthalene solid media.
Then secondary screening were done on 25 fungal is

    The study involved isolation and characterization of E.coli from patient’s infected with diarrhea , in order to study the ability of the bacteria to produce cytosine deaminase (CD). Result showed eight isolates of E.coli which showed adifference in the production of (CD) and the isolate of E. coli E33 was the beast of its production of CD than the other’s and the value of the specific activity was 4.920  u/mg protein , when grown in the medium which contains 1% glycerol ,3% peptone as a source of Carbon and Nitrogen respectively with pH 8.   The optimum cultural condition‘s for the production of CD from E. coli E33 was studied the result‘s  showed that the isolate gave the

     Bioethanol is an attractive fuel with higher potential for energy security and environmental safety. Olive solid residues were used as a raw material for the production of bioethanol through the use of different preliminary treatments . Separate treatments with cellulose, hydrochloric acid (HCl 5%), sulfuric acid (H₂SO₄ 2%), and liquid ammonia NH₄OH (20%) were used to convert cellulose and hemicellulose into monosaccharaides. The production of ethanol was observed during the fermentation process using R. minuta under anaerobic conditions.  After 3 days of fermentation, lowest concentrations of ethanol of  0.233, 0.249, 0.261, and 0.275 g/ l were produced from ol

The study aimed to determine of some Optimum conditions for bioremediation and removing of seven mineral elements included hexavalent chromium, nickel, cobalt, cadmium, lead, iron and copper as either alone or in group by living and heat treated cells of baker’s yeast Saccharomyces cerevisiae. The dried baker's yeast from Aldnaamaya China Company was used in this study. Biochemical tests was used to ensure yeast belonging to S. cerevisiae and then used to remove the mentioned mineral elementes under different conditions which included incubation period, pH, and temperature. It was found that the best of these conditions was 60 minutes for duration of incubation, 6 for pH, 25 ᵒC for temperature. During the study the behavior of living

Design of experiments (DOE) was made by Minitab software for the study of three factors used in the precipitation process of the Sodium Aluminate solution prepared from digestion of α-Al₂O₃  to determine the optimum conditions to a produce Boehmite which is used in production of ɤ-Al₂O₃ during drying and calcination processes, the factors are; the temperature of the sodium aluminate solution, concentration of HCl acid added for the precipitation and the pH of the solution at which the precipitation was ended. The design of the experiments leads to 18 experiments.

   The results show that the optimum conditions for the precipitation of the sodium aluminate solution which

The optimum conditions for production of fibrinolytic protease from an edible mushroom Pleurotus ostreatus grown on the solid medium , Sus medium, composed of Sus wastes (produced from extracted medicinal plant Glycyrrhiza glabra) were determined. Addition of 5% of Soya bean seeds meal in Sus medium recorded a maximum fibrinolytic protease activity resulting in 7.7 units / ml. The optimum moisture content of Sus medium supplemented with 5% Soya bean seeds meal was 60% resulting in 7.2 units / ml.Pleurotus ostreatus produced a maximum fibrinolytic protease activity when the spawn rate,pH of medium and incubation temperature were 2,6 and 30°C, respectively. The maximum fibrinolytic protease activity was 7.6 units / ml when incubat

A high-performance liquid chromatography method was employed for the quantitative determination of ascorbic acid (AA) which called vitamin C in three types of Iraqi citrus (orange mandarin and aurantium ) and to establish this goal , evaluation of ascorbic acid degradation is so important due to its significant criticality when exposure to ordinary atmospheric conditions. The chromatographic analysis of AA was carried out after their sequential elution with KH2PO4 ( as mobile phase) by reverse-phase HPLC technique with C8 column and UV detection at 214 nm. .Bad resolutions was appeared clearly for C8 column , so another alternative condition were carried out to improve the resolution by replacement of C8 by C18 column .Statistical treat