The aim of this paper is to determine the feasibility of using fluorometric methods as an indicator for quality and contamination of milk with E.coli bacteria, and selection the suitable wavelength to be used with laser induced auto fluorescence. Three groups of milk samples were used in this study: Fresh pasteurized milk samples, milk samples containing different concentration of E.coli bacteria which were added artificially, and milk samples that were kept in refrigerator for 3-5 days. Thirteen excitation wavelengths were used to get the emission spectra for all milk samples using spectroflourometer .The results showed that the emission spectra at 275nm excitation wavelength gave a good differentiation between these three groups. The data analysis demonstrated that the proposed method can bring progress in identification of milk quality and contamination with rapidness, high sensitivity and low cost diagnostic tool. Laser light at this wavelength that transmitted by optical fiber can be used for milk samples examination in situe and for more precise results.
A new, Simple, sensitive and accurate spectrophotometric methods have been developed for the determination of sulfamethoxazole (SMZ) drug in pure and dosage forms. This method based on the reaction of sulfamethoxazole (SMZ) with 1,2-napthoquinone-4-sulphonic acid (NQS) to form Nalkylamono naphthoquinone by replacement of the sulphonate group of the naphthoquinone sulphonic acid by an amino group. The colored chromogen shows absorption maximum at 460 nm. The optimum conditions of condensation reaction forms were investigated by (1) univariable method, by optimizing the effect of experimental variables (different bases, reagent concentration, borax concentration and reaction time), (2) central composite design (CCD) including the effect of
... Show MoreWatermelon has been reported to be vulnerable to insect pest pressure throughout its growth stages with a resultant indiscriminate calendar-based insecticide application (of up to 25 sprays/season in the study area), with its attendant consequences. In order to recommend the crop growth period(s) to effect chemical control measures that will give better return on investment, field trials were set-up in the early- and late-cropping seasons of 2016 and 2017. Forty, 5m long x 8m wide plots were demarcated in randomized complete block design in 4 replications. Treatments were applications of 0.5% Cyper-diforce® (Cypermethrin 30g/L + Dimethoate 250g/L) at seedling, mid-vegetative, mid-flowering, mid-frui
... Show MoreTight oil reservoirs have been a concerned of the oil industry due to their substantial influence on oil production. Due to their poor permeability, numerous problems are encountered while producing from tight reservoirs. Petrophysical and geomechanical rock properties are essential for understanding and assessing the fracability of reservoirs, especially tight reservoirs, to enhance permeability. In this study, Saadi B reservoir in Halfaya Iraqi oil field is considered as the main tight reservoir. Petrophysical and geomechanical properties have been estimated using full-set well logs for a vertical well that penetrates Saadi reservoir and validated with support of diagnostic fracture injection test data employing standard equations
... Show MoreRepeated blood transfusion in beta thalassemia major patients may lead to peroxidative tissue injury by secondary iron overload. In the present study, 100 patients(50 male+50 female) with beta thalassemia major patients with age (5-20) years and 60 healthy control were included during their attendance at Abin Al_Baladi hospital in Baghdad. Malondialdehyde ,Superoxide Dismutase and Vitamin E, were measured by using kits.The results showed A highly significant (p<0.01)increase in the levels of Malondialdehyde and Superoxide Dismutase, whereas, significant p(<0.01)decrease in the levels of vitamin-E, This suggest that oxidative stress and reduced antioxidant defense mechanism play an important role in pathogenesis of beta thalassemia
... Show MoreTwo different approaches, univariate and multivariate (simplex method), have been used to obtain the optimum conditions for the quantitative Spectrophotometric determination of Eu3+ using Solochrome violet RS (3-Hydroxy-4-(2-hydroxy phenyl azo) naphthalene -1sulfonic acid) (SVRS) as a chromogenic reagent. The investigation shows that Eu3+ ion forms a wine-red complex with SVRS in alkaline buffer solution having a maximum absorbance at 464 nm against reagent blank. Calibration graphs obtained under univariate and simplex were found to be linear in the range of (0.30-8.0) µg/ml with detection limit 0.061µg/ml and molar absorptivity of 9877.66 L/mol.cm and (0.40-10.0)µg/ml with
... Show MoreThe aim of this work is to determine the uranium concentration in soil samples
taken from the north, south, east, west and center of the city of Baghdad and
measure its impact on the rate of cell division for non-smokers peoples and living in
those areas and that between the ages 25-30 year.
The uranium concentration in the samples determined by using CR-39 track
detector . As calculated for the ten samples of each site when irradiated by thermal
neutrons from the (Am - Be) source with flux (5x 103 n S-1cm-2), the concentration
values were calculated by a comparison with standard geological samples. The
results indicate that the extent of the concentration of uranium in the soil north and
east of Baghdad was 12
The study area is located at AL Jabal AL-Akhdar region North Eastern part of Libya. The study includes (23) fresh samples that are taken from Darnah limestone Formation, these samples were distributed on and covered about (210) km2. The porosities and permeabilities of these samples were determined. This study is important because this formation is considered as a good reservoir for hydrocarbon accumulation in other places in Libya.
The porosity was measured by three different methods namely by porosimeter (using core samples), thin sections and binocular microscope. The permeability are also measured by two methods, the first method was by Nitrogen method and the other is by Klinkenberg method. The instrument that used for measuring
The present study was conducted to determine the optimum conditions required for lipase enzyme activity extracted from germinated sunflower seeds, including temperature, pH, agitation, time of incubation, enzyme concentration, substrate type, and concentrations of mineral salts and EDTA. Optimum pH, temperature and time of incubation required for lipase stability were also determined. The results showede optimum lipase activity (3.251U/ml) wasund at 30 ÌŠC and pH 7 after 20 minutes of incubation when using 1 ml lipase enzyme with 0.02 ml of CaCl2 (10 mM) at 100 rpm of agitation and in the presence of olive oil as the substrate for enzyme reaction. EDTA appeared to have inhibitory effects, while Ca+2 and Mg+2<
... Show MoreA newly photometric analytical method characterized by its speed and sensitivity was developed for the determination of folic acid in pure and pharmaceutical samples via its oxidation to reddish-orange coloured complex through oxidation by cerium (IV) sulphate in aqua medium using homemade Ayah 3Sx3-3D-solar cell CFI photometer. The colored species were determined using supper bright green light emitting diode (LED) as a source. A 100μl was taken as a best sample volume for the determination of folic acid. The linearity of calibration curve for the instrument response versus folic acid concentration was 0.005-20 mmol.L-1 while the L.O.D. was 0.5 mol.L-1 from the stepwise dilution for the minimum concentration of lowest concentration
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