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Detection and isolation of flavonoid and aromatic acid from Cynara scolymus different parts cultivated in iraq
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The target of this study was to study the natural phytochemical components of the head (capsule) of Cynara scolymus cultivated in Iraq. The head (capsule) of plant was extracted by maceration in70% ethanol for 72 hours, and fractioned by hexane, chloroform and ethyl acetate. Preliminary qualitative phytochemical screening was performed on the ethyl acetate fraction for capsule was revealed the presence of flavonoid and aromatic acids. These were examined by (high -performance liquid chromatography) (HPLC diodarray), (high- performance thin-layer chromatography)(HPTLC).

Flavonoids were isolated by preparative layer chromatography and aromatic acid was isolated by preparative high-performance liquid chromatography HPLC from the ethyl acetate fraction of capsule.

Then identified by High Performance Thin Layer Chromatography HPTLC, High performance liquid chromatography HPLC diode array , ultraviolet diode array UV-diode array and Liquid Chromatography /Mass Spectroscopy LC/MS. The chloroform fraction from the capsule was evaluated by Gas Chromatography//Mass Spectrometer(GC/MS). The different chromatographic and spectroscopic techniques revealed the presence of luteolin, apigenin and cinnamic acid in capsule of Cynara scolymus, also 9-octadecanoic acid (oleic acid), Oxalic acid, allyl tetradecyl ester, limonene, in chloroform of Cynara scolymus.

The results of the current study proved the presence of luleolinapigenin  and cinnamic acid in the ethyl acetate fraction of Cynara scolymus capsule.

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Publication Date
Sun Dec 25 2022
Journal Name
Iraqi Journal Of Pharmaceutical Sciences ( P-issn 1683 - 3597 E-issn 2521 - 3512)
Isolation of Catchin and Epigallocatchin From Iraqi Rhus coriaria By Preparative High-Performance Liquid Chromatography (PHPLC)
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 ABSTRACT

Two compounds were isolated from the fruit part of Rhus coriaria that grow wildly or cultivated in the north of Iraq. The compounds were separated by preparative high-Performance Liquid Chromatography and their structures were established based on detailed spectroscopic techniques like FTIR and LC-MS/MS.

Keywords: Rhus coriaria, Preparative HPLC, LC-MSMS, FTIR

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Publication Date
Sun Mar 01 2015
Journal Name
Baghdad Science Journal
Detection of Cronobacter sakazakii (Enterobacter sakazakii) in powdered food infants (PIF) and raw milk in Iraq
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This study was conducted to detect C.sakazakii PIF and raw milk. Two hundred samples of PIF were taken from the infected hospital infants who used this type of milk and from the local markets in addition to 16 sample of raw milk were collected. The study is the first to report the isolation of C. sakazakii and Enterobacter spp. from raw milk in Iraq. The distribution of C.sakazakii and Enterobacter spp. among the presumptive isolates using Vitek-GN2 system gave 1/16(6.25%) isolates of C.sakazakii and 4/16 (25%) isolates of Enterobacter spp. Enterobacter spp. isolates include (E.cloacae ssp. cloacae and E.cloacae ssp. dissolvens, E.hormaechei, and E.ludwigii) that isolate from raw milk Differences in between percentages of each isolate perse

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Publication Date
Sun Jan 01 2017
Journal Name
Iraqi Journal Of Medical Sciences
ISOLATION, IDENTIFICATION AND DETERMINATION OF ANTIFUNGAL SENSITIVITY OF FUNGI ISOLATED FROM A SAMPLE OF PATIENTS WITH RHINOSINUSITIS IN BAGHDAD CITY
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Publication Date
Tue Jan 01 2019
Journal Name
Indian Journal Of Public Health Research & Development
Isolation of CD34+ Human Melanocyte Stem Cells from Hair Follicles
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Publication Date
Fri Mar 01 2019
Journal Name
Journal Of Pharmaceutical Sciences And Research
Anatomical study of the vegetative parts and seeds of Vitex agnus-castus plant
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The species Vitex agnus-castus is an important medicinal plants and it is one of the cultivated and dicotyledon plants in Iraq. The anatomical of stem, petiole, midrib area, epidermis, veins, type of leaf stomata and seeds were studied by light microscope. In this research, shows the knowledge of anatomical characteristics of the studied plant, showing its importance as taxonomic characteristics through the sections of the stem, petiole, the midrib and the petals.

Publication Date
Mon Aug 01 2022
Journal Name
Biannual Journal By Department Of Linguistic And Translation Studies In Bayt Al Hikma
Metaphorical meaning of parts of the human body in Russian
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This article discusses some of the metaphorical use of language units. Here we will define the basic concepts and underline the causes of this phenomenon. Through research it is proven that through the application of names of some body parts achieves the variety of metaphorical meanings.

Publication Date
Mon Jan 01 2024
Journal Name
Journal Of Research In Pharmacy
Phytochemical constituents of ethyl acetate fraction of both roots and leaves of Sansevieria triafasciata cultivated in Iraq and assessment of its anti-proliferative effect on breast cancer cell line
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Publication Date
Fri Dec 01 2023
Journal Name
Iop Conference Series: Earth And Environmental Science
Detection of Genetic Relationship Between Eucalyptus Species in Iraq
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Abstract<p>Environmental factors that damage plant cells by dehydrating them, such cold, drought, and high salinity, are the most common environmental stresses that have an impact on plant growth, development, and productivity in cultivated regions around the world. Several types of plants have several drought, salinity, and cold inducible genes that make them tolerant to environmental challenges. The purpose of this study was to investigate several species in <italic>Eucalyptus</italic> plants and determined the evolutionary descent between different species of <italic>Eucalyptus</italic>. Samples from plants were used to extract genomic DNA. After sequence methods with phyl</p> ... Show More
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Publication Date
Thu Mar 25 1993
Journal Name
كلية العلوم. / جامعة بغداد
ISOLATION AND CHARACTERIZATION OF EXTREMELY HALOPHILIC BACTERIA FROM SADDAM RIVER REGIONS AND THE RULE OF MEMBRANE PROTEINS IN THE RESISTANCE TO HIGH SALINITY
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This study included isolation and characterization of extremely halophilic bacteria from Al-Massab Al-Aam region in South of Iraq Fifty isolates were identified by using numerical taxonomy 40 strains belonged to the genus Halobacterium which inclucted Hb. halobium Hb. cutirubrum Hb. salinarium Hb. saccharovorum Hb. valismortis and Hb. volcanii. Ten strains belonged to the genus Halococcus which included Hc. morrhuae Hc. saccharolyticus. Growth curves were sensitive mutants determined for wild type and salt Generation time in logarthmic phase was measured and found to be (10.37 2hr 7 0.59) for Hb. salinarium / 18 (6.490 hr 0.24) for Hb. cutirubrum / 32, (6.700 hr + 0.488) for Hb. valismortis / 20, (11.243 hr + 0.96) for Hb. volcanii / 7. (7

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Publication Date
Sun Aug 12 2018
Journal Name
Iraqi Journal Of Market Research And Consumer Protection
ISOLATION AND IDENTIFICATION OF PSEUDOMONAS SP THAT PRODUCE LIPASE: ISOLATION AND IDENTIFICATION OF PSEUDOMONAS SP THAT PRODUCE LIPASE
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15 local isolates of Pseudomonas were obtained from 35 samples from several sources such as soil, water and some high-fat foods. The ability of isolates to produce lipase was measured by the size of the clarification zone formed around the colonies on the lipase production medium and by measuring the enzymatic activity and specific enzymatic activity, the isolate M3 was found to be the most efficient for production of the enzyme, This isolate was identified by microscopic, morphological, some biochemical tests and genetic diagnosis of 16S gene sequences by using the (PCR) technique, and then comparing the results obtained with the National Center for Biotechnology Inform

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