Environmental factors that damage plant cells by dehydrating them, such cold, drought, and high salinity, are the most common environmental stresses that have an impact on plant growth, development, and productivity in cultivated regions around the world. Several types of plants have several drought, salinity, and cold inducible genes that make them tolerant to environmental challenges. The purpose of this study was to investigate several species in
Genus Eucalyptus belongs to the family Myrtaceae that consists of more than 700 species, various hybrids and varieties. The majorly distributed species that are grown in Iraq are Eucalyptus alba, E. macarthurii, E. siderophloia and E. camaldulensis, E. tereticornis, E. vicina. Most Eucalyptus species are highly dependent on rainfall, and this is challenged by climatic changes owing to global warming making it difficult to effectively match the availability of mature trees and the market demand, especially for use as power transmission poles. With the widespread availability of other naturally occurring Eucalyptus species, it has become important to determine the genetic diversity and to analyze the phenotypic tra
... Show MoreBackground: Klebsiella pneumoniae were considered as normal flora of skin, and intestine. It can cause damage to human lungs; the danger of this bacterium is related to exposure to the hospital surroundings. materials and methods: the detection of Klebsiella pneumoniae on morphological and biochemical tests and then assured with VITEK 2 system. Resistance to antibiotics was determined by Kirby-Baeur method. And genotyping of IMP-1 in isolates was done by PCR technique, then biofilm formation was identified by Micro titer plate method. Results: The present study included a collecting of 50 specimens from different clinical specimens, (blood 40%, urine 30%, sputum 20%, wound infection 10%); 10 isolates were identified as K
... Show MoreEssential oils of eucalyptus leaves and clove buds were isolated and tested for their bioherbicidal potential on different annual weed species. Gas Chromatography-Mass Spectrophotometry analysis (GC-MS) identified thirteen compounds representing around 87.11% of the total isolated eucalyptus oil. The main constituent was 1,8-cineole, which accounted for 68.15% of the total identified compounds. As for clove oil, eleven compounds were identified, representing 90.03% of the total compounds. Eugenol was the dominant compound and accounted for 73.89%. The bioherbicidal efficacy of the two oils and their combinations by three concentrations (2.5, 5, and 10%) were tested on four weedy species, namely Chenopodium album, Raph
... Show MoreThe human epidermal growth factor receptor-2 (HER2) gene plays a critical role in breast cancer development and progression. HER2 overexpression characterizes a biologically and clinically aggressive breast cancer subtype. In this study, 60 samples from Iraqi women with breast cancer were collected and investigated for HER2 protein in the tissue by immunohistochemistry. Also, 20 samples from healthy Iraqi women were used as a control. The results showed that 18 (30 %) patients expressed the HER2 protein. A molecular study for single nucleotide polymorphism (SNP) was conducted on samples metastasizing to lymph nodes. DNA was extracted and polymerase chain reaction (PCR) was performed to amplify e
... Show MoreThis study is a trail to know if the genes controlling some of heavy metals resistance ( lead, zinc, cadmium, cromium) in two types of pathogenic bacteria E. coli as gram negative bacteria and S. aureus as gram positive bacteria, present on the β-lactamase plasmid. Ten isolates of each bacterial types which produced β-lactamase enzyme, were cultivated in the presence of acridine orange. The growing in the presence of acridine orange resulted in loss of the β-lactamase genes in S. aureus and E. coli, and loss of the heavy metals resistance in S. aureus, while the resistance of E. coli against heavy metals still without any change. The results indicate that the genes for heavy
... Show MoreThere are growing concerns over the possibility of transfer genetically modified
sequences from genetically modified feed component (GM feed) to animals and
their products, moreover, affect these sequences on animal and human health. This
study was implemented to detect P35S in modified feed by using PCR technique by
detecting presence P35S promoter, which responsible for the regulation of gene
expression for most of the transgenic genes. Thirty eight feed samples were
collected from different sources of Baghdad markets, which have been used for
feeding livestock, comprise 21 coarse mixes feed, 13 pelleted feed, and 4 expanded
feed. Genomic DNA was extracted by using two methods, CTAB method and
Wizard kit. In
There are growing concerns over the possibility of transfer genetically modified sequences from genetically modified feed component (GM feed) to animals and their products, moreover, affect these sequences on animal and human health. This study was implemented to detect P35S in modified feed by using PCR technique by detecting presence P35S promoter, which responsible for the regulation of gene expression for most of the transgenic genes. Thirty eight feed samples were collected from different sources of Baghdad markets, which have been used for feeding livestock, comprise 21 coarse mixes feed, 13 pelleted feed, and 4 expanded feed. Genomic DNA was extracted by using two methods, CTAB method and Wizard kit. In order to verify the presenc
... Show MoreThe present paper attempts to establish a distinction between Exochomus negripennis (Er.) and E. quadripustulatus L., depending on the characters of femoral line, male genitalia and spermatheca.
Nowadays, the use of medicinal plants is being practiced at a wide range as a result of antibiotics resistant for the vast majority of microorganisms. Eucalyptus camaldulensis essential oil and hydrosol were used in this study against planktonic forms and biofilms of some species of Gram negative and Gram positive bacteria. The antibacterial and antibiofilm activities of this plant were detected using the microtiter plate and MIC approaches. The results proposed that the oil and hydrosol preparations have antibacterial activities against planktonic cells in different concentrations depending on the type of isolate. For antibiofilm activity, the results showed that E. camaldulensis oil was highly effective against bacter
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