Preferred Language
Articles
/
_RctUo8BVTCNdQwCEWv8
Genetic Detection of IMP-1 Gene and its Relationship with Biofilm Formation in Klebsiella pneumonia
...Show More Authors

Background: Klebsiella pneumoniae were considered as normal flora of skin, and intestine. It can cause damage to human lungs; the danger of this bacterium is related to exposure to the hospital surroundings. materials and methods: the detection of Klebsiella pneumoniae on morphological and biochemical tests and then assured with VITEK 2 system. Resistance to antibiotics was determined by Kirby-Baeur method. And genotyping of IMP-1 in isolates was done by PCR technique, then biofilm formation was identified by Micro titer plate method. Results: The present study included a collecting of 50 specimens from different clinical specimens, (blood 40%, urine 30%, sputum 20%, wound infection 10%); 10 isolates were identified as Klebsiella pneumoniae . All isolates, under study, developed high resistance toward Cefitrixon, Ampicillin, Amoxicillin, Ticarcillin, Ticarcillin+Clavulanic acid, and Ceftazidim estimated by disc diffusion method. All isolates characterized by harboring the highest resistant in a percentage reached 100% against antibiotics, under study. This study determined the Minimal Inhibitory Concentration were detected by eight E-test strips for isolates. As well as the isolates were strong biofilm production for three isolates, while three were moderate of biofilm formation and other isolates were weak former; at the value of (P≤0.05) was considered as a significant. Genotype detection of Metalo-beta lactamase (IMP-1) by PCR technique in Klebsiella pneumoniae . Upon using PCR technique exposed only three isolates;30% of isolates (two from urine, one from blood) samples harbored IMP-1 gene. The study was also found relationship between IMP-1 and biofilm formation in isolates which were harboring these genes, when (P ≤ 0.05). Conclusions: K. pneumoniae were isolated from different sources. All isolates were resistant to most antibiotics used in this study. The isolates have Metallo-beta lactamse. PCR was showed K. pneumoniae have IMP-1 gene,.This study also found there was relationship between biofilm formation and IMP-1 gene in K. pneumoniae (P≤0.05).  

Crossref
View Publication
Publication Date
Sat Jun 03 2023
Journal Name
Iraqi Journal Of Science
Detection of antibiotics resistance genes in clinical isolates of Klebsiella pneumonia
...Show More Authors

Total of 46 isolates of Klebsiella pneumoniae were collected from patients attending (Al-Yarmook Hospital and Education Labs / medical city), and isolates were re-identified, depending on morphology and biochemical tests . Disk diffusion method was employed to determine antibiotic susceptibility of forty six isolates by using eleven antibiotics .The results revealed the sensitivity of six isolates (9.3%) to Imipenem and Meropenem . On the other hand the isolates were showed 23.9% resistant against Ciprofloxacin, while some isolates shown higher resistant against several antimicrobial agents such as 65.2%, 69.0% for Amikacin and Cefepime consequently , 71.1%, 71.7 % for Amoxicillin -Clauvulanic acid and Gentamicin and 82.6% against Pipera

... Show More
View Publication
Publication Date
Sun Apr 30 2023
Journal Name
Iraqi Journal Of Science
Determination the Relationship between Some genetic Aspects with the Capsule Formation for Pathogenic Klebsiella pneumoniae Serotypes K1 &K2
...Show More Authors

A total of 47 samples were collected from different clinical specimens (urine, wounds, burns, sputum, blood, stools) during the period from November 2013 to
January 2014, only 18 isolates (38.29%) were identified as Klebsiella pneumoniae ,11isolates (23.4%) as E. coli , 9 isolates (19.1%) as S. aureus, 3isolates (6.3%) as Psedomonase spp., 2 isolates (4.2 %) as K. terrigena and 4 isolates (8.5%) as K. oxytoca. The results were shown the elevation of K. pneumoniae percentage among the bacterial isolates depending on cultural, microscopically, biochemically characteristics and confirmed by using the API 20E and VITEK 2 system . Also K. pneumoniae isolates were more frequently in sputum samples then burns, stools , urine, wounds ,and blo

... Show More
View Publication Preview PDF
Publication Date
Mon Apr 29 2024
Journal Name
Journal Of The College Of Basic Education
Detection Of Biofilm Formation By Beta- Lactam Resistance Klebsiella Pneumoniae Isolated From Clinical Specimens And Aquatic Samples
...Show More Authors

Preview PDF
Publication Date
Wed Dec 15 2021
Journal Name
Iraqi Journal Of Science
Study the Effect of some Medical Plants in Biofilm Formation and Antibiotic Sensitivity for Klebsiella Pneumoniae
...Show More Authors

Twenty clinical and fecal samples (ten clinical samples from patients attending to
Imam Ali Hospital and ten chicken faeces samples collected from local poultry farm
in Baghdad city) collected during December 2015, for isolated Klebsiella
pneumoniae. All K. pneumoniae isolates were extended-spectrum β- lactamase
producers and biofilm formation. The activities of two selected K. pneumoniae
isolates for their biofilm formation and susceptibility to antibiotics after treatment
with several plants extracts were investigated. The results of water and 60% ethanol
extracts for Matricaria chamomile flowers, Alhagi maurorum leafs, Syzygium
aromaticum buds (clove) and Arctium minus leafs were showed reduction of biofilm

... Show More
View Publication Preview PDF
Publication Date
Wed Feb 22 2023
Journal Name
Iraqi Journal Of Science
Detection of blaKPC Gene in Some Clinical Klebsiella pneumoniae Isolates in Baghdad
...Show More Authors

For the period from February 2014 till May 2014, one hundred and nine lactose fermenter clinical isolates from different samples (urine, stool, wound swab, blood, and sputum) were collected from Alyarmok, Alkadimiya, and Baghdad teaching hospitals at Baghdad governorate. Identification of all Klebsiella pneumoniae isolates were carried out depending on macroscopic, microscopic characterizations, conventional biochemical tests, and Api 20E system. Fifty-three (48.62%) isolates represented K. pneumoniae; however, 51.73% represented other bacteria. Susceptibility test was achieved to all fifty-three K. pneumoniae isolates using five antibiotic disks (Ceftazidime, Ceftriaxone, Cefotaxime, Imipenem, and Meropenem). Most of tested isolates (90

... Show More
View Publication Preview PDF
Publication Date
Fri Mar 27 2020
Journal Name
Iraqi Journal Of Science
Colistin Susceptibility in Carbapenem Resistant Klebsiella Pneumoniae and their Ability of Biofilm Formation
...Show More Authors

A total of 157 clinical samples were collected from different clinical specimens (urine, sputum, blood, swabs, and cannula) from several hospitals in Iraq. Among the samples, 51 isolates (32.48%) of Klebsiella pneumoniae were identified according to morphologicaland cultural characteristics as well as the Enterosystem 18R test. Higher numbers of K. pneumoniae isolates were observed in urine samples (26, 52%) than the other samples, and in females (70.6%) than males (29.4%) (female: male ratio of about 2.4:1). Antibiotic susceptibility of K. pneumoniae against 12 commonly used antibiotics was determined through the disc-diffusion method. The results revealed a higher resistance rate in 51 isolates (100%) against Cephalexin, followed by Ce

... Show More
View Publication Preview PDF
Scopus (3)
Crossref (1)
Scopus Crossref
Publication Date
Fri Dec 20 2024
Journal Name
Baghdad Science Journal
Interference with quorum sensing of Klebsiella pneumoniae by some plant extracts can affect the biofilm formation and antibiotic resistance.
...Show More Authors

Quorum sensing (QS) is a perfectly orchestrated molecular communication system. It is a boon for Klebsiella pneumoniae, and bane for the host. This system is believed to make K. pneumoniae a leading cause of multidrug-resistant (MDR) nosocomial infections. This study aimed to investigate the antibacterial and anti-biofilm potential of medicinal plant extracts through interfering with QS of K. pneumoniae. The effect of different concentrations of ethanolic extracts of cinnamon and clove on K. pneumoniae was determined by analyzing the growth curve, survival assay (MTT), Qualitative and quantitative biofilm formation, antibiotic resistance, along with studying gene expression of the genes encoding the above traits, using quantitative real tim

... Show More
View Publication Preview PDF
Publication Date
Mon Apr 10 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
Role of Extracted Genomic DNA on Biofilm Formation by Pseudomonas aeruginosa and Klebsiella pneumoniae in vitro
...Show More Authors

   Bacteria form complex and highly elaborate surface adherent communities known as biofilms.Biofilm have been shown to be associated with several human diseases ,and to colonize a wide variety of medical devices . The current study focuses on contribution of extracted genomic   DNA in  biofilm formation by   P. aeruginosa and  K. pneumoniae isolates   .The percentages of Pseudomonas aeruginosa recovery from drinking water  in this study were  10%(20 positive P. aeruginosa  samples ) and K. pneumonia.,  7%(14 positive K. pneumonia samples).The results showed that all P.aeruginosa and K. pneumoniae isolates (100%) were slime producer but in different degrees by forming  of black

... Show More
View Publication Preview PDF
Publication Date
Tue Dec 29 2020
Journal Name
Iraqi Journal Of Science
Detection of icaA Gene Expression in Clinical Biofilm-Producing Staphylococcus Aureus Isolates
...Show More Authors

The pathogenicity resulting from Staphylococcus aureus infection has remarkable importance as one of the community-associated bacterial infections, due to the virulent ability of these bacteria to produce biofilms. This study was designed to detect biofilm production in clinical isolates from samples of wounds and urinary tract infections. The expression levels of the icaA gene that is responsible of slime layer production in biofilms was compared in isolates with different biofilm producing capabilities. Fifty seven samples that included 32 samples from urine and 25 samples from wounds were collected from Alwasti Hospital, Al-Kindi Teaching Hospital, and Alzahraa Clinic, Baghdad, Iraq. The bacteria was identified accor

... Show More
View Publication Preview PDF
Scopus (6)
Crossref (2)
Scopus Crossref
Publication Date
Fri Nov 24 2023
Journal Name
Iraqi Journal Of Science
Crystal Violet Binding Assay for Assessment of Biofilm Formation by Klebsiella pneumoniae on Catheter, Glass and Stainless-steel Surfaces
...Show More Authors

In this paper, quantified study of the biofilm formed by Klebsiella pneumoniae isolated from urine specimen of patient suffering from acute urinary tract infection (UTI) on catheter, stainless-steel and glass coupon surfaces, as well as determine the relationship between time contact and biofilm progression using crystal-violet binding assay based on the values of optical density at 620nm of the crystal violet stain which bonded total biofilm biomass by resolubizing with 99.9% ethanol at the specific interval times. The result showed biofilm formed on three tested surfaces but in different degrees. According to obtained data, the catheter coupons presents a higher capability to attract bacteria cell and biofilm formation followed by glas

... Show More
View Publication Preview PDF