Protease enzyme production was studied and optimized as a first step to collect information about solid state fermenter) to produce protease enzyme. A local isolated Aspergillus niger was used for this study with constant spores feeding in every experiment at (105/g). Experiments carried out in conical flasks with (250 ml) containing (10 g) of wheat straw as a substrate with different conditions included temperature, pH, hydration ratio, and fermentation time, the results comprised by measuring protease activity (u). The results showed that the best activity can be obtained at (T = 32°C, t= 100 hrs, pH= 2.5 and hydration ratio is 1:3). On the other hand the results is courage to proceed to design a solid state protease fermenter from wheat straw.
Select 30 isolate from Bacillus to detect the ability to produce pullulanase enzyme in liquid and solid state fermentation, and use the isolate Bacillus licheniformis (Bs18) because the highest production of enzyme, the optimum condition for the production of enzyme by liquid state fermentation (LSF) in growen with: media contains starch + pullulan as a carbon source, peptone as a nitrogen source, inoculums size 2 ml, and incubated at 40 C° with pH 7 for 48 hrs. In addition pullulanase production by solid state fermentation (SSF) was investigated using isolated Bacillus licheniformis (Bs18). Optimization of process parameters were carried out ,the optimum solid substrate , Temperature , pH , incubation period , inoculation size , hydrat
... Show MoreRadiological assessment due to existing of natural occurring radioactive materials
(NORM) in South Rumaila oil field was achieved in this study. Different samples
including soil, sludge, scale, oil, and water were collected from different stages of
oil and gas production in Markazia Degassing Station (SDS) in South Rumaila oil
field. Radioactivity of Ra-226, Th-232 and K-40 were measured using gamma
spectrometry system based on HPGe detector with efficiency of 30%. The results
show that some locations within SDS are contaminated with NORM. The activity of
Ra-226, Th-232 and K-40 range between 18.4 to 312.8, 9.4 to 140.8 and 66.4 to
800.8 (Bq/kg) respectively. The places to be more contaminated among the other
p
!'hi_, i1rycsligation was carried ou1 dn J)Ct'iphcral blom.l s_amplc:s.
wl1ich wendrawi·1 ih)rl1 patients w.ith l)1).hoid !'ever. Fifteen palic111 nging ]5- 45 years old .<iS vvdl as ten sample::. w:cr: c·ollec ted from healthy persons-al the same range of age. Sera were used t'or csti niation the act i vity and sp ci fie activity of t\LJ.A. The resuJts sho:«"'d sig11ltl'c u1t increase i11&
... Show More
(8)
A pot culture experiment was conducted at the greenhouse of soil and water resources department in College of Agriculture, University of Baghdad in Abo-Ghraib at season 2009-2010 to investigate the effects of using foliar application of some macro and micronutrients in induce antioxidant enzymes in wheat grown under salt stress . Doar85 planted under three levels of salt stress, and three combinations of foliar application were used from nutrients (K+ Ca) at 3000 and 1500 mg.L-1 respectively, and (Fe + Zn + Mn) at 30, 20, and 10 mg.L-1 respectively , and ( K+ Ca) + (Fe+ Zn + Mn). The results showed that increasing levels of sodium chloride in the irrigation of water significantly increased at p<0.05 level SOD and POD activity
... Show More
Field experiment was conducted by using two fertilization systems (i.e.) biofertilizers (inoculation with Pseudomonas putida and with Azotobacter chroococcum and non - inoculation) and chemical fertilization (100%, 50% and 25% of recommended by Ministry of Agriculture) to study the influence of these system and interaction on water and grain yield productivity, some growth phytohorones and number of bacterial cells in soil rizosphere of root of wheat crop under water scarcity. The result showed that the integrate fertilization (inoculation with Pseudomonas putida and Azotobacter chroococcum bacterial + 50% of the recommended chemical fertilizer) recorded 5.70 and 5.55 t ha-1, respectively with reducing the chemical fertilizer app
... Show More
(6)
The present study aims to detect CTX-M-type ESBL from Escherichia coli clinical isolates and to analyze their antibotic susceptibility patterns. One hundred of E. coli isolates were collected from different clinical samples from a tertiary hospital. ESBL positivity was determined by the disk diffusion method. PCR used for amplification of CTX-M-type ESBL produced by E. coli. Out of 100 E. coli isolates, twenty-four isolates (24%) were ESBL-producers. E. coli isolated from pus was the most frequent clinical specimen that produced ESBL (41.66%) followed by urine (34.21%), respiratory (22.23%), and blood (19.05%). After PCR amplification of these 24 isolates, 10 (41.66%) isolates were found to possess CTX-M genes. The CTX-M type ESBL
... Show More
(3)
(1)
Biogas is one of the most important sources of renewable energy and is considered as an environment friendly energy source. The major goal of this research is to see if rice husk (Rh) waste and pomegranate peels (PP) waste are suitable for anaerobic digestion and what effect NaOH pre-treatment has on biogas generation. Rice husk and pomegranate peels were tested in anaerobic digestion under patch anaerobic conditions as separate wastes as well as blended together in equal proportions. The cumulative biogas output for the blank test (no pretreatment) was 1923 and 2526 ml, respectively using a single rice husk (Rh) and pomegranate peel (PP) substrates. The 50% rice husk digestion and 50% of pomegranate peels for blank test gave the result 224
... Show MoreIndustrial development has recently increased, including that of plastic industries. Since plastic has a very long analytical life, it will cause environmental pollution, so studies have resorted to reusing recycled waste plastic (sustainable plastic) to produce environmentally friendly concrete (green concrete). In this research, producing environmentally friendly load-bearing concrete masonry units (blocks) was considered where five concrete mixtures were compressed at the blocks producing machine. The cement content reduced from 400 kg/m3 (B-400) to 300 kg/m3 (B-300) then to 200 kg/m3 (B-200). While (B-380) was produced using 380 kg/m3 cement and 20 kg/m3 nano-sil
... Show More
(2)
The experimentes were carried out by using a nutrient solution in the green house of Biology Dept , college of Education (Ibn Al- Haitham , University of Baghdad .) during 2001 -2002 growing season in order to determine the effect of sodium ion, by using four concentrations: (zero , 40,80,120. Mm), to study the appearance and physiological characteristics for two wheat varieties : IP A99 and Al-Kaid according to factorial experiment in completely randomized design for each concentration. The rcsults showed that the fourth concentration (120 Mm) for the Na ion is the most effective one on Leaf area, chlorophyll content, soluble carbohydrates, nitrogen content and protein percentage. Moreove
... Show More