Preferred Language
Articles
/
_hZ0TocBVTCNdQwCP0PQ
Gentamicin Upregulates the Gene Expression of hla and nuc in Staphylococcus aureus
...Show More Authors

The current study aimed to detect the effect of gentamicin stress on the expression of hla (encodes hemolysin) and nuc (encodes nuclease) genes of Staphylococcus aureus. Fifty-eight isolates identified as S. aureus were isolated locally from different clinical specimens. Disk diffusion method was used to detect the resistance to S. aureus. The minimum inhibitory concentration (MIC) of gentamicin was estimated by broth microdilution method. hla and nuc genes were determined by polymerase chain reaction technique. The biofilm was evaluated using the microtiter plate method in the presence and absence of gentamicin at sub-MIC. The results showed that 18 (31%) and 40 (69%) S. aureus isolates were sensitive and resistant to gentamicin, respectively. All S. aureus isolates succeeded in forming biofilm. However, eight (13.79%), 28 (48.28%) and 22 (37.93%) isolates produced weak, moderate and strong biofilms respectively. In most isolates, gentamicin at sub-MIC decreased biofilm intensity. Due to gentamicin stress, the hla and nuc genes were upregulated in S. aureus biofilm.

Publication Date
Mon Jan 23 2023
Journal Name
The Egyptian Journal Of Hospital Medicine
Estimation of SLC25A3 Gene Expression in Chronic Myelogenous Leukemia Iraqi Patients
...Show More Authors

Background: Chronic myelogenous leukemia is a malignant hematological disease of hematopoietic stem cells. It is difficult to adapt treatment to each patient's risk level because there are currently few clinical tests and no molecular diagnostics that may predict a patient's clock for the advancement of CML at the time of chronic phase diagnosis. Biomarkers that can differentiate people based on the outcome at diagnosis are needed for blast crisis prevention and response improvement. Objective: This study is an effort to exploit the SLC25A3 gene as a potential biomarker for CML. Methods: RT-qPCR was applied to assess the expression levels of the SLC25A3 gene. Results: In comparison to the mean ΔCt of the control group, which was found to b

... Show More
Publication Date
Sun Jan 26 2025
Journal Name
Journal Of Baghdad College Of Dentistry
Antimicrobial activity of different types of mouthwashes against Streptococcus mutans, Staphylococcus aureus and Candida albicans (In vitro study)
...Show More Authors

Background: Recently increasing number of people are using mouthwashes for general and oral care while the primary appeal of a mouthwash is an aid to breath freshness and cleansing the mouth, the majority of mouthwashes also claim to have antiseptic properties. The aim of this study is to determine the antimicrobial effectiveness of eight types of mouthwashes against Streptococcus mutans, Staphylococcus aureus and Candida albicans in vitro. Materials and methods: Agar diffusion technique was used to evaluate the antimicrobial activity of eight types of mouthwashes against Streptococcus mutans, Staphylococcus aureus and Candida albicans isolated from the oral cavities of patients attending dental clinics at college of dentistry - Baghdad Uni

... Show More
View Publication Preview PDF
Publication Date
Wed May 25 2022
Journal Name
Iraqi Journal Of Science
The Correlation Between the Persistence of Methicillin Resistant Staphylococcus Aureus Isolates to Mupirocine and Toxin-Antitoxin Type II Genes
...Show More Authors

      Under high concentrations of antibiotics, a fraction of the bacterial population exhibits a phenomenon known as persistence. Toxin- system (TA system) has been reported to be involved in the formation of E. coli, Mycobacterium, and S. aureus persisters.  In this study, the ability of thirty Iraqi isolates of MRSA to form in vitro persister cells after exposure to three different antibiotics (Ceftriaxone 30 µg, Mecillinam 10 µg, and Mupirocin 20 µg) was examined by TD test. Additionally, efflux pump inhibitor [Fluphenazine 0.25 mg/ml] was combined with the antibiotic that triggered persister formation. The distribution of mazEF and yefM-yoeB (Type II TA system) in the tested isolates was detected by PCR. 91% of Mupirocin su

... Show More
View Publication Preview PDF
Scopus Crossref
Publication Date
Thu Jul 03 2008
Journal Name
مجلة التقني المجلد الحادي والعشرون العدد3
THE EFFECT OF ULTRA VIOLET, SOME AMINO ACIDS AND VITAMINS ON THE SUSCEPTIBILITY TO SOME ANTIBIOTICS AGAINST STAPHYLOCOCCUS AUREUS
...Show More Authors

Abstract: This research was performed to study the effect of some amino acids and vitamins on the growth of bacteria Staphylococcus aureas and its sensitivity against UV light. The results showed low inhibition in bacterial growth because amino acids repairs the damges caused by UV light. Besides the effect of two groups of antibiotics (β-lactame and tetracycline) on the growth of S. aureus and the possible interference of amino acids and vitamins in the activity of the antibiotics against this bacteria in the presence of UV light were studied. The result show increase in the sensitivity towards these antibiotics and provided protection against the antibiotics.

Publication Date
Sun Jun 07 2009
Journal Name
Baghdad Science Journal
Changes of the susceptibility of Staphylococcus aureus bacteria to the local therapeutic agent by using Nitrogen laser
...Show More Authors

This study involves the investigation of the effect of nitrogen laser with 337.1 nm wavelength on the sensitivity of Staphylococcus aureus bacteria by using local therapeutic due to burns. Thirty six isolate of Staphylococcus aureus bacteria were isolated from 25 patients suffering from sever burns, each isolate of bacteria was irradiated with nitrogen laser at (5, 10, 15 and 30) pulses/second repetition rates for 1, 5, 10, 20 and 30 minutes for each repetition rate. The effects of nitrogen laser on the local therapeutics sensitivity of bacteria were obtained using Kirby Baur method. Changes in the sensitivity of bacteria to local therapeutics (Tetracyclin, Chloramphenicol, Flumizin and Fucidin) occur at high repetition rate(30 pulses/seco

... Show More
View Publication Preview PDF
Crossref
Publication Date
Fri Jun 30 2023
Journal Name
Iraqi Journal Of Science
Expression of Protamine-1 Gene and Association with Interleukin-12 gene in Infertile Men: A Case-Control Study in Iraqi Patients
...Show More Authors

     Approximately 15% of all couples all over the world suffer from difficulty conceiving their first child. The word "infertility" is used to describe this problem.

When a couple had  regular, unprotected sexual intercourse for a year or longer, it indicates that they have been unsuccessful in their efforts to conceive.

      The current investigation aims to find out if there is a relationship between PROTAMIN-1(PRM-1) and INTERLEUKINE-12 (IL-12) gene expressions and their effect on the development of infertility. The current investigation comprised 100 teratozoospermia patients and 100 healthy fertile controls who had their semen examined. Samples were given by Al-Nahrain University's Biotech

... Show More
View Publication
Scopus Crossref
Publication Date
Tue Jul 12 2016
Journal Name
World Journal Of Experimental Biosciences
Effect of honey and sodium bicarbonate on formation inhibition and removal of Staphylococcus aureus and Klebsiella pneumoniae biofilm in vitro
...Show More Authors

Publication Date
Wed Apr 19 2017
Journal Name
Ibn Al-haitham Journal For Pure And Applied Sciences
The Effect of Aqueous Extract and Essential Oils of the Leaves of Eucalyptus incrassate on β- Lactam Resistant Staphylococcus aureus
...Show More Authors

According to the extraction procedure , Eucalyptus incrassata leave sample yielded 5% and 2% w/w(Based on dry leaves ) of the aqueous extract and essential oils respectively. Disk diffusion method was used to determine the antimicrobial activity of aqueous extract and essential oils of E . incrassata leaves against eight isolates of multidrug- resistant of Staphylococcus aureus ( S. aureus) . It was found that aqueous extract and essential oils have variable antimicrobial activity(the inhibition zone diameter ranged from 7 to 14 mm respectively ) , while essential oils showed more effect than aqueous extract .         It was noticed that values of Minimal Inhibitory Concentration ( MIC )  for

... Show More
View Publication Preview PDF
Publication Date
Tue Mar 30 2021
Journal Name
Iraqi Journal Of Science
Expression of IRS1 Gene in Pregnant Women with Gestational Diabetes Mellitus, in The Third Trimester
...Show More Authors

To study the genetic effect of gestational diabetes mellitus by study IRS1gene expression in female with Gestational diabetes mellitus. It is characterized high level of blood glucose, especially during first trimester then increased during the 2nd and 3rd trimester of the pregnancy period. The blood samples taken from one hundred twenty healthy women and female with gestational diabetes mellitus in 3rd trimester period of pregnancy, level of fasting blood glucose (FBG) also HbA1c% measured to diagnose GDM, in addition to lipid profile (cholesterol, triglyceride, HDL, LDL, and VLDL), molecular study consist of RNA extraction and qRT- PCR for IRS1gene expression determination. The fasting blood glucose mg/

... Show More
View Publication Preview PDF
Scopus (1)
Crossref (1)
Scopus Crossref
Publication Date
Thu Oct 01 2020
Journal Name
Sys Rev Pharm
Role of plant growth regulators in gene expression of SGR gene responsible for stay green of wheat varieties
...Show More Authors

A field experiment was carried out during winter season of 2019-2020 at Al-Mhanawyah Research Station - Agriculture Research Directorate - Babylon Governorate / Iraqi, to study the gene expression of Sgr gene responsible for controlling the duration of staying green in varieties of wheat under effect of plant growth regulator during the two growth stages (vegetative and reproductive) by using quantitative reverse transcription-PCR (RT-qPCR) technique and achieving the highest grain yield for a number of wheat varieties. Randomized complete block design (RCBD) arranged according to split plots used with three replicates. The experiment included twelve wheat varieties (Saberbic, Al-Rasheed, Iraq, Tamoz-3, Al-Adnaniya, Babel, IPA-99, Al-Latife

... Show More
Scopus (7)
Scopus