Antibiotic resistance increment is a major problem for the human society nowadays which encourages the efforts to look for new therapeutic alternatives from natural defenses. Synergistic antibacterial activity of epidermin and staphylolysin LasA A against Staphylococcus aureus (Staph aureus), Escherichia coli (E. coli) and Pseudomonas aeruginosa (Ps. aeruginosa) was evaluated. The antibacterial activities of epidermin from Staphylococcus epidermidis (Staph epidermidis) and Staphylolysin (LasA) from Ps. aeruginosa using the agar well diffusion assay were evaluated, and then using the micro dilution method to evaluate the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC). The checkerboard method and fractional inhibitory concentration (FIC) were used to evaluate the combination between epidermin and LasA toward targeted clinical isolates of Staph aureus, E. coli and Ps. aeruginosa. The results revealed a synergistic effect between epidermin and LasA on all clinical isolates growth. The highest MIC and MBC of epidermin were 36.04 µL/mL and 51.73 µL/mL against Staph aureus; meanwhile, the highest MIC and MBC of LasA were 44.38 µL/mL and 50 µL/mL against Staph aureus. The FICindex revealed synergistic interactions in combination of epidermin and LasA which recorded 0.286 for Staph aureus while for E. coli was 0.327 and for Ps. aeruginosa was 0.390 respectively showing a synergism effect. This study finds that combination of epidermin with LasA had inhibitory activity on the targeted clinical isolate growth, which can be useful for designing and developing alternative therapeutic strategies against pathogens causing wound and burn infections.
A total of nine swab samples were collected from inflamed teeth and gingiva of human’soral cavity from a dentist clinic in Baghdad. All specimens were cultured in Mitis Salivarius agar medium and the isolated bacterial pure colonies werethen identified by using VITEK2. Three samples were diagnosed and identified as Staphylococcus lentus. One of the three isolates which showed a distinctive heavy growth on the media was selected for further analysis in this study. Paper disk diffusion method was used to detect the antibacterial activityof three of mouthwash solutions (Zak, Colgate and Listerine). The results showed that “Colgate†was the most active solution with antibacterial activity compared with the other two soluti
... Show MoreThe Antimicrobial activity of Probiotic Bifidobacterium sp and Prebiotics : chicory roots ( Hot water extract ) and Inulin ( 10 % ) against some Pathogenic bacteria (Esherichia coli , Proteus mirabilis , Klebsiella sp , Pseudomonas aeruginosa , Serratia marcescens was studied . The combination of Probiotic and Prebiotics (Synbiotic) (Bifidobacterium sp + chicory ) and (Bifidobacterium sp + Inulin ) also tested for their antimicrobial activity against Pathogenic bacteria . Results showed that Bifidobacterium sp had good antimicrobial activity against all the Pathogenic bacteria tested , followed by chicory and inulin . The synergistic inhibitory effect of Synbiotic (Bifidobacterium sp + chicory ) and ( Bifidobacterium sp + inuli
... Show MoreThe manganese doped zinc sulfide nanoparticles were synthesized by simple aqueous chemical reaction of manganese chloride, zinc acetate and thioacitamide in aqueous solution. Thioglycolic acid is used as capping agent for controlling the nanoparticle size. The main advantage of the ZnS:Mn nanoparticles of diameter ~ 2.73 nm is that the sample is prepared by using non-toxic precursors in a cost effective and eco-friendly way. The structural, morphological and chemical composition of the nanoparticles have been investigated by X-ray diffraction (XRD), Scanning Electron Microscopy (SEM) with energy dispersion spectroscopy (EDS) and Fourier transform infrared (FTIR) spectroscopy. The nanosize of the prepared nanoparticles was elucidated by Scan
... Show MoreThe antibacterial activity of Capparis spinosa L. extracts obtained from Baghdad was evaluated against six clinical bacteria isolates of Helicobacter pylori. The results presented in this work demonstrated that the leaves extract showed a significant effect against bacteria as compared to the root and fruit extracts at 100 mg/ml concentration, with inhibition zone ranging from 12.0 to 30.7 mm in each bacteria strain. The end results of GC-MS analysis indicated that the ethanol extracts of caper have a lot of active chemical compounds, including twenty-five, eighty-two and sixty-eight phytoconstituent compounds, that are distinguished in the extracts of roots, leaves and fruits with C. spinosa L. respectively. I
... Show MoreABSTRACT: Pathogenic bacteria responsible for the causation of many common diseases have been identified on currency notes. The present investigation was carried out on one hundred currency notes of all the denominations (50, 100, 250, 500 and 1000RY), obtained from different occupational mainly bus drivers, hawker street, vegetable vendor, restaurants and butchers and fish seller groups in Taiz city,Yemen. Identification and characterization revealed active participation of the following species of organisms in the ascending order of percentage as E. coli(50.28 %),Staphylococci aureus(14.04 %), Klebsiellaspp(4.39 %),proteus(4.39 %), salmonella(1.25 %), shigella(0.72 %), Coagulase negative staphylococcus(0.60 %), pseudomonas(0.50 %),
... Show MoreOver the past few decades, the health benefits are under threat as many commonly used antibiotics have become less and less effective against certain illnesses not only because many of them produce toxic reactions but also due to the emergence of drug-resistant bacteria. The clinical use of a combination of antibiotic therapy for Pseudomonas aeruginosa infections is probably more effective than monotherapy. The present study aims to estimate the antibacterial and antibiofilm activity of Conocarpus erectus leaves extracts against multi-drug resistant P. aeruginosa isolated from different hospitals in Baghdad city. One hundred fifty different clinical specimens were collected from patients from September 2021 to January 2022. All samples were
... Show MoreThe entire investigation's focus was on the production of nickel oxide nanoparticles (NiONPs), using prodigiosin pigments produced by Serratia marcescens as a stabilizing and reducing agent. Nickel oxide nanoparticles are synthesized using nickel sulfate NiSO4 (10mg) with a concentration of prodigiosin (10g/100ml). Biosynthesized NiO nanoparticles have been characterized by using many techniques, such as (UV-Vis, AFM, XRD, FTIR, and FE-SEM). The AFM analysis revealed that NiONPs have an average diameter size of (41.77 mm), and the FE-SEM Image displays Spherical. Additionally, the effect of NiONPs with different concentrations on the bacteria Pseudomonas aeruginosa was measured and the inhibition
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