This study included an analysis of three stations (Al Dora, Al Za'franiya, and Arab Ejbur) chosen to study the Physiochemical and microorganism (Fungi and Bacteria) loud of the Tigris River in the southern section of Baghdad city. The result of this research shows that the highest temperature recorded in summer in Al Za'franiya was 37Co, while the lowest temperature recorded in winter in Al Dora was 9Co. and the value of pH recorded the highest in summer it was 7.9 in Arab Ejbur, and the lowest value was in winter 7.1 in Al Dora regions, While Total Organic Carbon (TOC) shows the highest values found in the summer was 6.7 Mg L-1in Al Za'franiya Samples, and the lowest values were 2.0 Mg L-1in Arab Ejbur during the winter. The more frequently isolated fungi were Aspergillus niger, and the lowest frequently was Chrysosporium was observed during the survey period. At the same time, Bacteriological analysis of water resources showed that Escherichia coli and Pseudomonas aeruginosa were more frequently isolated bacteria and the lowest frequently bacteria were Klebsiella ornithinolytica and Proteus mirabilis. Keywords. Fungi, Bacteria, Temperature, TOC, pH, Tigris River.