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Synergistic Antibacterial and Anticancer Effects of a Biohybrid Composed of Zinc Oxide Nanoparticles and <i>Streptococcus Salivarius</i> Bacteriocin
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ABSTRACT<p> Antimicrobial therapies are desperately needed since the threat posed by multidrug‐resistant (MDR) bacteria only grows. Bacteriocins produced by <italic>Streptococcus salivarius</italic> possess promising antibacterial properties, yet their practical application is hindered by low production yield and limited stability. Here, we report the green synthesis of a novel biohybrid by conjugating purified <italic>S. salivarius</italic> bacteriocins with zinc oxide nanoparticles (ZnONPs), aiming to enhance bacteriocin stability and antimicrobial efficacy. The successful creation of well‐dispersed nanohybrids was validated by thorough characterization using x‐ray diffraction (XRD), transmission electron microscopy (TEM), and scanning electron microscopy (SEM), exhibiting predominantly cubic and spherical morphologies with sizes ranging from 8 to 68 nm. The ZnONPs–bacteriocin nanohybrids demonstrated pronounced antibacterial activity against both Gram‐positive and Gram‐negative MDR pathogens, as evidenced by significant inhibition zones, and low values for the minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC). In vitro cytotoxicity, assays revealed a dose‐dependent reduction in the viability of both cancerous (A375) and normal (HdFn) cell lines. The nanohybrid displayed greater cytotoxicity toward A375 cells (IC <sub>50</sub> = 121.7 µg/mL) than HdFn cells (IC <sub>50</sub> = 161.6 µg/mL), indicating a degree of selectivity. Collectively, our results demonstrate that green‐synthesized ZnONPs–bacteriocin biohybrids are eco‐friendly and exhibit potent, broad‐spectrum antimicrobial and selective cytotoxic activities, suggesting their significant potential for biomedical applications targeting resistant bacterial infections and malignant cells. </p>
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Publication Date
Thu Oct 01 2020
Journal Name
Systematic Reviews In Pharmacy
Estimation of activity and toxicity of silver nanoparticles loaded metronidazole against Giardia lamblia
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Giardia lamblia is one of most common protozoan cause diarrheas, and the most health problem in development countries worldwide. Our work aimed to assess activity and toxicity of metronidazole loaded silver nanoparticles in treatment of acute giardiasis in mice. After inoculated mice with Giardia cysts in a dose of 105 cyst for acute infection, treatments were given for eight days. Number Giardia cysts in stool were discovered. Toxicity nanoparticles was estimated by Measurement oxidative stress markers (GSH) and (MDA) in liver, kidney tissue homogenate. The results showed single therapy was better effect by silver nanoparticles, highest percentages of reduction in number of cysts Giardia lamblia of infected mice treated with silver nanopar

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Publication Date
Fri Jun 14 2019
Journal Name
Biotechnology Letters
Extracellular biosynthesis of silver nanoparticles from Penicillium italicum and its antioxidant, antimicrobial and cytotoxicity activities
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Publication Date
Tue Jun 20 2023
Journal Name
Baghdad Science Journal
Synthesis and Evaluation of Platinum Nanoparticles Using F. Carica Fruit Extract and Their Antimicrobial Activities
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Abstract

In this manuscript, a simple new method for the green synthesis of platinum nanoparticles (Pt NPs) utilizing F. carica Fig extract as reducing agent for antimicrobial activities was reported. Simultaneously, the microstructural and morphological features of the synthesized Pt NPs were thoroughly investigated. In particular, the attained Pt NPs exhibited spherical shape with diameter range of 5-30 nm and root mean square of 9.48 nm using Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM), respectively. Additionally, the final product (Pt NPs) was screened as antifungal and antibacterial agent against Candida and Aspergillus species as well as Gram-positive Staphyllococcus aureus and G

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Publication Date
Mon Sep 19 2022
Journal Name
International Journal Of Biomaterials
Green Biosynthesis of Silver Nanoparticles from Moringa oleifera Leaves and Its Antimicrobial and Cytotoxicity Activities
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The plant occupied the largest area in the biosynthesis of silver nanoparticles, especially the medicinal plants, and it has shown great potential in biotechnology applications. In this study, green synthesis of silver nanoparticles from Moringa oleifera leaves extract and its antifungal and antitumor activities were investigated. The formation of silver nanoparticles was observed after 1 hour of preparation color changing. The ultraviolet and visible spectrum, Fourier transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, and transmission electron microscopy techniques were used to characterize synthesis particles. Ultraviolet and visible spectroscopy showed a silver surface plasmon resonance band at 434

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Publication Date
Tue Jan 02 2018
Journal Name
Journal Of Educational And Psychological Researches
Family violence, its causes and effects on society (women and children)
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Summary of the research Family violence, its causes and effects on society (women and children). Search Goal: The current research aims to identify: 1. Causes of family and social violence (for children and women). 2. How to process. research importance : Domestic violence is a major and devastating problem that has a negative and direct impact on children in particular and women or mothers in general. The problem of domestic violence is linked to many factors and individual, social, economic, psychological and environmental variables, which makes one theory that claims to be inaccurate. It is difficult to understand the nature of family violence without linking it to some of the concepts related to it. The negative effects that may lead

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Publication Date
Tue Feb 12 2019
Journal Name
Biochemical And Cellular Archives
TOXIC EFFECTS OF ABRUS PRECATORIUS EXTRACT ON LIVER AND KIDNEY OF ALBINO MICE
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Abrus precatorius L. is an important medicinal plant belonging to family Fabaceae. The present study was conducted to perform pre-clinical safety evaluation and study the pharmacological effects of the ethanolic seeds extract of A. precatorius in management of pain, psychiatric and neurological conditions. Acute toxicity was performed to study the general behavioural pattern of mice after treatment with the test extract (single doses of 1000, 2000, 3000,4000,5000 mg/kg, body weight) and sub-acute toxicity studies were performed to study the toxic effects of the test extract (1000,2000,3000 mg/kg, per os for 14 days) on different haematological parameters, body and organ weight and histopathology of liver and kidney. The toxicological evalua

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Publication Date
Wed Nov 01 2023
Journal Name
Iraqi Journal Of Pharmaceutical Sciences ( P-issn 1683 - 3597 E-issn 2521 - 3512)
Possible Effects of Vitamin D3 and Levofloxacin on Selected Hematology Parameter of Rats
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Vitamin D is a fat-soluble vitamin with antioxidant and DNA protecting properties , Levofloxacin is a member of the fluoroquinolone drug class, Its broad-spectrum bactericidal effect affects both Gram-positive and Gram-negative bacteria.

The goal of the study is to analyze the haematology analysis in rats received levofloxacin and show the preventive impact of vitamin D3 by analyzing the haematology parameters: packed cell volume (PCV), mean corpuscular hemoglobin concentration(MCHC),haemoglobin (HB), red blood cell (RBC), mean corpuscular volume (MCV),meancorpuscular haemoglobin(MCH), WBC ,differential WBC, and Platelets.

The study included 42 rats divided into 6 groups each group 7 rats. group I negative control

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Publication Date
Mon Oct 01 2018
Journal Name
Plant Archives
Effects of bacterocin from MRSA and pseudomonas aeruginosaagainst biofilm of food born pathogen
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Publication Date
Sun Mar 06 2016
Journal Name
Baghdad Science Journal
Addition of Some Primary and Secondary Amines to Graphene Oxide, and Studying Their Effect on Increasing its Electrical Properties
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Previously many properties of graphene oxide in the field of medicine, biological environment and in the field of energy have been studied. This diversity in properties is due to the possibility of modification on the composition of this Nano compound, where the Graphene oxide is capable of more modification via addition other functional groups on its surface or at the edges of the sheet. The reason for this modification possibility is that the Sp3 hybridization (tetrahedral structure) of the carbon atoms in graphene oxide, and it contains many oxygenic functional groups that are able to reac with other groups. In this research the effect of addition of some amine compounds on electrical properties of graphene oxide has been studied by the

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Publication Date
Sun Sep 07 2014
Journal Name
Baghdad Science Journal
Determination of Copper and Lead and Study their Poisonous Effects in Drinking Water in Baghdad
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The objective of this study is to determine the concentration of copper and lead (mg/L) in drinking water by using absorption spectrophotometic and Atomic Absorption spectrophotometric method from different area in Baghdad and with different intervals , The results show that the concentration of copper and Lead ( mgL) in tap water which remains motionless in plumbing system for following periods one hours, 3 hours, 6 hours, 12 hours, 24 hours, 7 days and 14 days are (1 , 2.2 , 4 , 5.3 , 7.5 , 10 and 16 mgL copper ) & ( 0.3, 0.5 , 0.8 , 1 , 2.5 , 3 , 3.8 mg /L lead ) respectively .from these results its clear that high levels of copper & Lead occur if tap water comes in contact with copper - lead plumbing and copper lead -containing fix

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